p39, a major baculovirus structural protein: Immunocytochemical characterization and genetic location

Pearson, Margot; Russell, Rebecca L. Q.; Rohrmann, George F.; Beaudreau, G. S.

doi:10.1016/0042-6822(88)90102-x

Cited by 37 publications

(37 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Last, 18 of these nudiviral genes, corresponding to baculovirus core genes [29], should perform essential functions of the virus cycle, based on functional characterization in baculoviruses [30] (table 1). Most viral functions [10,27] such as transcription, particle assembly and packaging and entry into host cells could be identified (table 1): (i) all RNA polymerase subunits involved in baculovirus transcription; (ii) genes encoding the equivalent of the major baculovirus capsid (VP39) [31,32] and a protein involved in nucleocapsid assembly (38K) [33]; (iii) all components of the baculovirus PIF complex involved in cell entry [34]. No transcripts involved in viral DNA replication could be identified, except for a nudiviral helicase gene in Microplitis demolitor [26].…”

Section: (B) Bracoviruses Originate From An Ancestral Nudivirus (I) Nmentioning

confidence: 99%

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Herniou

Huguet

Thézé

et al. 2013

Phil. Trans. R. Soc. B

152

172

View full text Add to dashboard Cite

International audienceThe Polydnaviridae (PDV), including the Bracovirus (BV) and Ichnovirus genera, originated from the integration of unrelated viruses in the genomes of two parasitoid wasp lineages, in a remarkable example of convergent evolution. Functionally active PDVs represent the most compelling evolutionary success among endogenous viral elements (EVEs). BV evolved from the domestication by braconid wasps of a nudivirus 100 Ma. The nudivirus genome has become an EVE involved in BV particle production but is not encapsidated. Instead, BV genomes have co-opted virulence genes, used by the wasps to control the immunity and development of their hosts. Gene transfers and duplications have shaped BV genomes, now encoding hundreds of genes. Phylogenomic studies suggest that BVs contribute largely to wasp diversification and adaptation to their hosts. A genome evolution model explains how multidirectional wasp adaptation to different host species could have fostered PDV genome extension. Integrative studies linking ecological data on the wasp to genomic analyses should provide new insights into the adaptive role of particular BV genes. Forthcoming genomic advances should also indicate if the associations between endoparasitoid wasps and symbiotic viruses evolved because of their particularly intimate interactions with their hosts, or if similar domesticated EVEs could be uncovered in other parasites

show abstract

Section: (B) Bracoviruses Originate From An Ancestral Nudivirus (I) Nmentioning

confidence: 99%

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Herniou

Huguet

Thézé

et al. 2013

Phil. Trans. R. Soc. B

152

172

View full text Add to dashboard Cite

show abstract

“…This stroma consists of two distinct regions: one is a fibrillar electron-dense area, while the other is an electron-lucent intrastromal space in which progeny nucleocapsids are formed (Summers, 1971;Harrap, 1972;Young et al, 1993). A major capsid protein of 39 kDa, VP39 (Pearson et al, 1988;Blissard et al, 1989;Thiem & Miller, 1989), has been shown to accumulate in this intrastromal space (Braunagel et al, 1996a). In contrast to the intrastromal space, the electron-dense region is considered to be DNA rich and can be intensively stained with DNA-specific fluorescent dyes (Williams & Faulkner, 1997;Rosas-Acosta et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Kawasaki

Matsumoto

Nagamine

2004

Journal of General Virology

View full text Add to dashboard Cite

IE1, a principal transcriptional activator of the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV), is an essential factor for viral DNA replication. During viral infection, IE1 accumulates in discrete subnuclear structures where viral DNA replication occurs. To analyse the dynamic properties of IE1, we monitored green fluorescent protein-tagged IE1 (IE1-GFP) in BmNPV-infected B. mori cells by live-cell microscopy. Time-lapse imaging showed that IE1-associated structures gradually expanded and occasionally fused with one another, while photobleaching experiments revealed that IE1-GFP was relatively immobile inside the IE1-associated structures. To investigate the spatial relationships between IE1 and viral structural proteins in infected cells, three GFP-tagged viral components were expressed together with DsRed-tagged IE1. Two structural proteins that constitute the occlusion-derived virus (ODV), P91-GFP and GFP-ODV-E25, localized to the periphery of the IE1-associated structures. While local accumulations of these proteins were often in contact with the IE1-associated structures, they did not extend beyond the boundaries of the structures. In contrast, the major capsid protein VP39-GFP predominantly accumulated within the IE1-associated structures. These data indicated, in conjunction with the finding of a high DNA content in the structures, that IE1 localizes to the virogenic stroma and therefore support the prediction previously proposed that the virogenic stroma is a site for viral DNA replication as well as for the assembly of nucleocapsids.

show abstract

“…P10 appears to play a role in the proper assembly of polyhedra, as it has been demonstrated that p 10-minus virus recombinants produce polyhedra which are unstable and readily fracture (Williams et al, 1989), with polyhedron envelopes that are incomplete or absent (Vlak et al, 1988;Williams et al, 1989). The p39 protein is a component of the virion nucleocapsid and is one of the major proteins occluded within polyhedra (Pearson et al, 1988;Thiem & Miller, 1989). All four of these polyhedron-associated proteins are expressed as late or very late genes and all demonstrate nuclear localization except pl0, which appears to be located in both the nucleus and cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

“…), with p39 showing intense nuclear localization by 48 h p.i. using immunofluorescent staining (Quant-Russell et al, 1987;Pearson et al, 1988). Polyhedrin is not observed on Western blots until 48 h p.i.…”

Section: Introductionmentioning

confidence: 99%

Immunoelectron microscopic examination of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus-infected Lymantria dispar cells: time course and localization of major polyhedron-associated proteins

Russell

Pearson²,

Rohrmann³

1991

Journal of General Virology

View full text Add to dashboard Cite

Immnnoelectron microscopy was employed to examine the temporal expression and localization of two proteins involved in baculovirus polyhedron assembly (polyhedrin and p 10) of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) in infected Lymantria dispar cells. In addition, the association of p 10 with the polyhedron envelope (PE) protein was studied. The major capsid protein (p39) was also examined to investigate the association of virion structural proteins with polyhedron formation. In infected cells, p39 did not show a concentrated association with any infected-cell structures other than nucleocapsids and appeared to be randomly distributed over the nucleocapsid surface. Likewise, polyhedrin showed no major concentrations outside of developing or mature polyhedra. The pl0 antibody cross-reacted with a protein associated with condensed chromosomes in uninfected cells. In infected cells, pl0 is a component of the body of fibrillar structures. The PE protein has been shown to accumulate around the periphery of fibrillar structures. Cells infected with a polyhedrin-minus virus expressing the fl-galactosidase gene under the control of the polyhedrin promoter were examined to determine whether the lack of polyhedra would influence the localization of major polyhedronassociated viral proteins. High concentrations of PE protein accumulating on the periphery of fibrillar structures appeared to be the major difference from wild-type virus-infected cells. The fl-galactosidase protein appeared to be distributed throughout the nucleus and cytoplasm, in contrast with the specific localization of the viral proteins.

show abstract

p39, a major baculovirus structural protein: Immunocytochemical characterization and genetic location

Cited by 37 publications

References 16 publications

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

When parasitic wasps hijacked viruses: genomic and functional evolution of polydnaviruses

Analysis of baculovirus IE1 in living cells: dynamics and spatial relationships to viral structural proteins

Immunoelectron microscopic examination of Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus-infected Lymantria dispar cells: time course and localization of major polyhedron-associated proteins

Contact Info

Product

Resources

About