P38 - Saisonnalité et biométéorologie de l’infarctus du myocarde en Côte-d’Or (France)

Laaidi, Mohamed; Zeller, Marianne; Wagner, V.; Cotin, Y.; Tertre, Alain Le; Laaidi, Karine; Besancenot, Jean‐Pierre

doi:10.1016/s0398-7620(05)84660-7

Cited by 1 publication

(1 citation statement)

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“…Cypress pollinosis, like most allergies occurring in winter (e.g. involving indoor allergens), may lead to upper respiratory tract infections and aggravated manifestations such as rhinosinusitis and rhino-bronchitis [3]. However, despite the severity of symptoms, this seasonal pathology is still underestimated for various reasons, including: (i) its pollination often coincides with the cold or flu season and many people suffer through winter without knowing they are sensitized to cypress pollen [4], and (ii) the dubious efficiency of current cypress pollen allergic extracts in the diagnosis of true cypress pollen allergy [4][5][6].…”

Section: Cypress Pollen Allergymentioning

confidence: 99%

Proteomics of cypress pollen allergens using double and triple one‐dimensional electrophoresis

Shahali¹,

Sutra²,

Haddad³

et al. 2012

Electrophoresis

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Italian cypress (Cupressus sempervirens, Cups) pollen causes allergic diseases in inhabitants of many of the cities surrounding the Mediterranean basin. However, allergens of Cups pollen are still poorly known. We introduce here a novel proteomic approach based on double one-dimensional gel electrophoresis (D1-DE) as an alternative to the 2-DE immunoblot, for the specific IgE screening of allergenic proteins from pollen extracts. The sequential one-dimensional combination of IEF and SDS-PAGE associated with IgE immunoblotting allows a versatile multiplexed immunochemical analysis of selected groups of allergens by converting a single protein spot into an extended protein band. Moreover, the method appears to be valuable for MS/MS identification, without protein purification, of a new Cups pollen allergen at 43 kDa. D1-DE immunoblotting revealed that the prevalence of IgE sensitization to this allergen belonging to the polygalacturonase (PG) family was 70% in tested French allergic patients. In subsequent triple one-dimensional gel electrophoresis, the Cups pollen PG was shown to promote lectin-based protein-protein interactions. Therefore, D1-DE could be used in routine work as a convenient alternative to 2-DE immunoblotting for the simultaneous screening of allergenic components under identical experimental conditions, thereby saving considerable amounts of sera and allergen extracts.

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