p38-mediated cell growth and survival drive rapid embryonic wound repair

Scepanovic, Gordana; Hunter, Miranda V.; Kafri, Ran; Fernández-González, Rodrigo

doi:10.1016/j.celrep.2021.109874

Cited by 16 publications

(14 citation statements)

References 103 publications

(136 reference statements)

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“…Recent data revealed that these cells might be doing more than simply stretching: cells adjacent to epidermal wounds in axolotl, zebrafish, and Drosophila embryos increase their volume in a process necessary for rapid wound healing ( Tanner et al. , 2009 ; Kennard and Theriot, 2020 ; Scepanovic et al. , 2021 ).…”

Section: Do the Materials Properties Of Tissues Affect Aj Remodeling ...mentioning

confidence: 99%

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Powering morphogenesis: multiscale challenges at the interface of cell adhesion and the cytoskeleton

Fernández-González

Peifer

2022

MBoC

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Among the defining features of the animal kingdom is the ability of cells to change shape and move. This underlies embryonic and postembryonic development, tissue homeostasis, regeneration, and wound healing. Cell shape change and motility require linkage of the cell’s force-generating machinery to the plasma membrane at cell–cell and cell–extracellular matrix junctions. Connections of the actomyosin cytoskeleton to cell–cell adherens junctions need to be both resilient and dynamic, preventing tissue disruption during the dramatic events of embryonic morphogenesis. In the past decade, new insights radically altered the earlier simple paradigm that suggested simple linear linkage via the cadherin–catenin complex as the molecular mechanism of junction–cytoskeleton interaction. In this Perspective we provide a brief overview of our current state of knowledge and then focus on selected examples highlighting what we view as the major unanswered questions in our field and the approaches that offer exciting new insights at multiple scales from atomic structure to tissue mechanics.

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Section: Do the Materials Properties Of Tissues Affect Aj Remodeling ...mentioning

confidence: 99%

“…, 2011 ; Xu and Chisholm, 2011 ; Holt et al. , 2021 ; Scepanovic et al. , 2021 ), the development of activity sensors to track the dynamics of channel activation during wound repair ( Stewart et al.…”

Section: Do the Materials Properties Of Tissues Affect Aj Remodeling ...mentioning

confidence: 99%

Powering morphogenesis: multiscale challenges at the interface of cell adhesion and the cytoskeleton

Fernández-González

Peifer

2022

MBoC

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“…Axolotl skin explants heal slower in the presence of hypertonic media (Tanner et al, 2009). With the opposite perturbation, hypotonic shock, zebrafish larvae and Drosophila embryos heal faster (Gault et al, 2014; Kennard & Theriot, 2020; Scepanovic, Hunter, Kafri, & Fernandez‐Gonzalez, 2021). Zebrafish, as a freshwater species, are thought to use such hypotonic shock as a normal signal for migration after wounding.…”

Section: Discussionmentioning

confidence: 99%

Confined keratocytes mimic in vivo migration and reveal volume‐speed relationship

2023

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Fish basal epidermal cells, known as keratocytes, are well‐suited for cell migration studies. In vitro, isolated keratocytes adopt a stereotyped shape with a large fan‐shaped lamellipodium and a nearly spherical cell body. However, in their native in vivo environment, these cells adopt a significantly different shape during their rapid migration toward wounds. Within the epidermis, keratocytes experience two‐dimensional (2D) confinement between the outer epidermal cell layer and the basement membrane; these two deformable surfaces constrain keratocyte cell bodies to be flatter in vivo than in isolation. In vivo keratocytes also exhibit a relative elongation of the front‐to‐back axis and substantially more lamellipodial ruffling, as compared to isolated cells. We have explored the effects of 2D confinement, separated from other in vivo environmental cues, by overlaying isolated cells with an agarose hydrogel with occasional spacers, or with a ceiling made of polydimethylsiloxane (PDMS) elastomer. Under these conditions, isolated keratocytes more closely resemble the in vivo migratory shape phenotype, displaying a flatter apical‐basal axis and a longer front‐to‐back axis than unconfined keratocytes. We propose that 2D confinement contributes to multiple dimensions of in vivo keratocyte shape determination. Further analysis demonstrates that confinement causes a synchronous 20% decrease in both cell speed and volume. Interestingly, we were able to replicate the 20% decrease in speed using a sorbitol hypertonic shock to shrink the cell volume, which did not affect other aspects of cell shape. Collectively, our results suggest that environmentally imposed changes in cell volume may influence cell migration speed, potentially by perturbing physical properties of the cytoplasm.

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“…Axolotl skin explants heal slower in the presence of hypertonic media (Tanner et al, 2009). With the opposite perturbation, hypotonic shock, zebrafish larvae and Drosophila embryos heal faster (Gault et al, 2014; Kennard & Theriot, 2020; Scepanovic et al, 2021). Zebrafish, as a freshwater species, are thought to use such hypotonic shock as a normal signal for migration after wounding.…”

Section: Discussionmentioning

confidence: 99%

Confined keratocytes mimic in vivo migration and reveal volume-speed relationship

Labuz

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Theriot

2022

Preprint

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Fish basal epidermal cells, known as keratocytes, are well-suited for cell migration studies. In vitro, isolated keratocytes adopt a stereotyped shape with a large fan-shaped lamellipodium and a nearly spherical cell body. However, in their native in vivo environment, these cells adopt a significantly different shape during their rapid migration towards wounds. Within the epidermis, keratocytes experience 2D confinement between the outer epidermal cell layer and the basement membrane; these two deformable surfaces constrain keratocyte cell bodies to be flatter in vivo than in isolation. In vivo keratocytes also exhibit a relative elongation of the front-to-back axis and substantially more lamellipodial ruffling, as compared to isolated cells. We have explored the effects of 2D confinement, separated from other in vivo environmental cues, by overlaying isolated cells with an agarose hydrogel with occasional spacers, or with a ceiling made of PDMS elastomer. Under these conditions, isolated keratocytes more closely resemble the in vivo migratory shape phenotype, displaying a flatter apical-basal axis and a longer front-to-back axis than unconfined keratocytes. We propose that 2D confinement contributes to multiple dimensions of in vivo keratocyte shape determination. Further analysis demonstrates that confinement causes a synchronous 20% decrease in both cell speed and volume. Interestingly, we were able to replicate the 20% decrease in speed using a sorbitol hypertonic shock to shrink the cell volume, which did not affect other aspects of cell shape. Collectively, our results suggest that environmentally imposed changes in cell volume may influence cell migration speed, potentially by perturbing physical properties of the cytoplasm.

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p38-mediated cell growth and survival drive rapid embryonic wound repair

Cited by 16 publications

References 103 publications

Powering morphogenesis: multiscale challenges at the interface of cell adhesion and the cytoskeleton

Powering morphogenesis: multiscale challenges at the interface of cell adhesion and the cytoskeleton

Confined keratocytes mimic in vivo migration and reveal volume‐speed relationship

Confined keratocytes mimic in vivo migration and reveal volume-speed relationship

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