p38-avidity-ELISA: examination of herds experiencing epidemic or endemic Neospora caninum-associated bovine abortion

Schares, Gereon; Bärwald, Andrea; Staubach, Christoph; Söndgen, P.; Rauser, M.; Schröder, Ronald; Peters, Martin C.; Wurm, R.; Selhorst, Thomas; Conraths, Franz J.

doi:10.1016/s0304-4017(02)00103-6

Cited by 46 publications

(32 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The antibody titres obtained with and without urea are then used to calculate the IgG avidity index (titre with urea multiplied by 100 divided by titre without urea). Low avidity values are related with recent infection and high avidity values are observed in animals with chronic infection (Jenkins et al 2000;McAllister et al 2000;Schares et al 2002;Björkman et al 2003Björkman et al , 2005Aguado-Martínez et al 2005;Frössling et al 2005). However, up to date this technique does not allow to discriminate a recrudescence or a re-infection from a chronic infection (Aguado-Martínez et al 2005).…”

Section: Techniquesmentioning

confidence: 99%

Diagnosis of bovine neosporosis: Recent advances and perspectives

Ortega‐Mora

Fernández-García

Gómez-Bautista

2006

Acta Parasitologica

View full text Add to dashboard Cite

Neospora caninum is considered a major cause of abortion in cattle. Appropriate techniques for diagnosis of bovine neosporosis, both in vivo and in aborted foetuses, have been developed in the last ten years and some of them are commercially available. For diagnosis in live animals, detection of antibodies in serum or milk has been shown to be the best option both at the herd and the individual level. These techniques are excellent tools to examine N. caninum-associated abortion problems and to adopt some basic herd-control measures. Concerning foetal diagnosis, detection of compatible lesions by histological examination and parasites by PCR in brain (as well as heart and liver) are the best choices. Diagnostic criteria to distinguish foetal infection and Neospora-associated abortion are based not only on the demonstration of the parasite in the foetus but also on the extent and severity of the lesions in the foetus, foetal age and the assessment of neosporosis at the herd level. In the near future, new tools to diagnose infection should help to detect animals with parasite reactivation by testing the immune response to stage-specific antigens and lead to the development of molecular typing methods to characterise different parasite isolates. Finally, uniform diagnostic procedures need to be established between laboratories and countries in order to standardise result interpretation. The role of National or Regional Reference Laboratories is essential in countries or regions where control programmes for the disease are being developed.

show abstract

Section: Techniquesmentioning

confidence: 99%

Diagnosis of bovine neosporosis: Recent advances and perspectives

Ortega‐Mora

Fernández-García

Gómez-Bautista

2006

Acta Parasitologica

View full text Add to dashboard Cite

show abstract

“…There are reports that in the years after an epidemic abortion outbreak, the affected herd may experience endemic abortions (56,309,352). Abortion outbreaks have been defined as epidemic if the abortion outbreak is temporary and if 15% of the cows at risk abort within 4 weeks, 12.5% of the cows abort within 8 weeks, and 10% of the cows abort within 6 weeks (309,399,488). In contrast, an abortion problem is regarded as endemic if it persists in the herd for several months or years.…”

Section: Risk Factors For Bovine Neosporosismentioning

confidence: 99%

Epidemiology and Control of Neosporosis andNeospora caninum

2007

View full text Add to dashboard Cite

SUMMARY Neospora caninum is a protozoan parasite of animals. Until 1988, it was misidentified as Toxoplasma gondii. Since its first recognition in dogs in 1984 and the description of the new genus and species Neospora caninum in 1988, neosporosis has emerged as a serious disease of cattle and dogs worldwide. Abortions and neonatal mortality are a major problem in livestock operations, and neosporosis is a major cause of abortion in cattle. Although antibodies to N. caninum have been reported, the parasite has not been detected in human tissues. Thus, the zoonotic potential is uncertain. This review is focused mainly on the epidemiology and control of neosporosis in cattle, but worldwide seroprevalences of N. caninum in animals and humans are tabulated. The role of wildlife in the life cycle of N. caninum and strategies for the control of neosporosis in cattle are discussed.

show abstract

“…The completion of the epidemiological cycle occurs when dogs ingest infected tissues-aborted fetus or placenta tissue-forming oocysts all over again (5). Drinking water and food, contaminated by dogs or any other carriers not yet identified can cause this infection (6,7).…”

Section: Dogs Have Been Identified As Definitive Hostsmentioning

confidence: 99%

Seroprevalence and risk factors associated to Neospora caninum in dairy cattle herds in the municipality of Pasto, Colombia

2013

Rev MVZ Córdoba

View full text Add to dashboard Cite

Seroprevalencia y factores de riesgo asociados a RESUMENObjetivo. Determinar la seropositividad de N. caninum en bovinos no vacunados contra el síndrome reproductivo y los factores de riesgo asociados a la presentación de esta enfermedad en los hatos lecheros del municipio de Pasto, Nariño. Materiales y métodos. Se seleccionaron fincas sobre los 2527 msnm. Se analizaron muestras de suero de 238 vacas Holstein mediante la prueba de ELISA indirecta para determinar la seropositividad a N. caninum y se realizó una encuesta epidemiológica incluyendo variables sobre el ganado como: medidas sanitarias y de manejo. Se realizó un análisis multivariado mediante regresión logística binaria con un intervalo de confianza del 95% (p<0.05) utilizando el programa SPSS19 ® . Resultados. La prevalencia estimada para N. caninum fue de 76.9 %. Los factores de riesgo asociados a Neosporosis en las fincas estudiadas son los residuos de abortos, que no se entierran y se dejan a la intemperie (OR 5.49; IC 95% 1.7-17.7), alimentar los perros con desperdicios (OR 15.44 IC 95% 1.94-123.22) y la monta directa (OR 14.62 IC 95% 1.55-137.53). Conclusiones. La elevada prevalencia de N. caninum y la tasas bajas de abortos en el municipio de Pasto, confirma que la enfermedad no se presenta en todos los animales positivos serológicamente, pero sugiere que en algún momento de su vida fueron expuestos al agente causal. A partir de los factores de riesgo identificados en este trabajo se pueden establecer recomendaciones para un control efectivo de enfermedades reproductivas como la Neosporosis presentes en la región.

show abstract

p38-avidity-ELISA: examination of herds experiencing epidemic or endemic Neospora caninum-associated bovine abortion

Cited by 46 publications

References 19 publications

Diagnosis of bovine neosporosis: Recent advances and perspectives

Diagnosis of bovine neosporosis: Recent advances and perspectives

Epidemiology and Control of Neosporosis andNeospora caninum

Seroprevalence and risk factors associated to Neospora caninum in dairy cattle herds in the municipality of Pasto, Colombia

Contact Info

Product

Resources

About