P2Z adenosine triphosphate receptor activity in cultured human monocyte- derived macrophages

Hickman, SE; Khoury, Joseph El; Greenberg, Steven; Schieren, Ira; Silverstein, Saul

doi:10.1182/blood.v84.8.2452.bloodjournal8482452

Cited by 13 publications

(7 citation statements)

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“…Like adenosine, these effects are mediated via specific cell membrane receptors. There are at least three different classes of ATP receptors, termed P2U/P2Y (G-protein-coupled, acting through release of Ca++ and phospholipase C), P2X (acting directly to activate ATP-ligand gated cation channels), and P2Z receptors, which mediate the opening of large "pores" in plasma membranes, making cells permeable to ions and molecules up to 900 daltons (90). The ATP receptors are encoded by a very large number of genes.…”

Section: Role Of Autotaxin In Tumour Motilitymentioning

confidence: 99%

Ecto‐phosphodiesterase/pyrophosphatase of lymphocytes and non‐lymphoid cells: structure and function of the PC‐1 family

Goding

Terkeltaub

Maurice

et al. 1998

Immunological Reviews

148

180

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Many developmentally regulated membrane proteins of lymphocytes are ecto-enzymes, with their active sites on the external surface of the cell. These enzymes commonly have peptidase, phosphodiesterase or nucleotidase activity. Their biological roles are just beginning to be discovered. Although their expression is usually associated with particular stages of lymphoid differentiation, the same gene products are often expressed on the surface of certain non-lymphoid cell types outside the immune system, indicating that their functions cannot be unique to lymphocytes, nor can they be ubiquitous. The plasma cell membrane protein PC-1 (phosphodiesterase I; EC 3.1.4.1/nucleotide pyrophosphatase; EC 3.6.1.9), which was one of the first serological markers for lymphocyte subsets to be discovered, is a typical example. Within the immune system, PC-1 is confined to plasma cells, which represent about 0.1% of lymphocytes. However, PC-1 is also expressed on cells of the distal convoluted tubule of the kidney, chondrocytes, osteoblasts, epididymis and hepatocytes. Recent work has shown that PC-1 is a member of a multigene family of ecto-phosphodiesterases that currently has two other members, PD-1 alpha (autotaxin) and PD-1 beta (B10). Within this family, the extracellular domains are highly conserved, especially around the active site. In contrast, the transmembrane and cytoplasmic domains are highly divergent. Individual members of the eco-phosphodiesterase family have distinct patterns of distribution in different cell types, and even within the same cell. For example, PC-1 is present only on the basolateral surface of hepatocytes, while B10 (PD-1 beta) is confined to the apical surface. Analysis of conservation and differences in the sequence of their cytoplasmic tails may illuminate intracellular targetting signals. Ecto-phosphodiesterases may play a part in diverse activities in different tissues, including recycling of nucleotides. They may also regulate the concentration of pharmacologically active extracellular compounds such as adenosine or its derivatives and cell motility. Some members may modulate local concentrations of pyrophosphate, and hence influence calcification in bone and cartilage.

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Section: Role Of Autotaxin In Tumour Motilitymentioning

confidence: 99%

Ecto‐phosphodiesterase/pyrophosphatase of lymphocytes and non‐lymphoid cells: structure and function of the PC‐1 family

Goding

Terkeltaub

Maurice

et al. 1998

Immunological Reviews

148

180

View full text Add to dashboard Cite

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“…The phenomenon of ATP-induced cell membrane permeabilization has been shown, and partly characterized, in a variety of cell types [1,2,8,14,20]. Although it is generally accepted that the pore-forming P2z receptor, for which ATP 4serves as a ligand, plays a major role in the permeabilization t~,rocess, the mechanisms underlying this process are only i,artly understood [3,12,[21][22][23].…”

Section: Discussionmentioning

confidence: 99%

“…The P2z is also a porefirming receptor in certain cells [4,[6][7][8] ,'/-methylene]triphosphate; TPP +, tetraphenylphosphonium; A0, the electrical potential across the cell membrane; 860, difference in A~ cell types, P2x in excitatory cells and P2z in certain cell types, like transformed and hemopoietic cells [8]. ATP-induced cell membrane permeabilization has been shown in many cells, including transformed mouse fibroblasts [2], mast cells [9], erythroleukemia cells [10], macrophages [11,12], Novikoff hepatoma cells [13], and certain other cells [8,14]. It is accepted by many investigators that permeabilization is mediated by the P2z receptor, but the mechanisms underlying this process are only partly understood [8].…”

Section: Introductionmentioning

confidence: 99%

Combined effects of ATP and its analogs on the membrane permeability in transformed mouse fibroblasts

Arav

Friedberg

1996

FEBS Letters

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Extracellular ATP (0.6 mM) induces a marked decrease in the membrane potential, followed by an increase in cell membrane permeability in transformed mouse fibroblasts. The effects of the ATP analogs, pICHaIppA and p[NH]ppA (0.6 m VI), on the membrane potential and permeability are much less pronounced. ATP at 0.05 mM has no effect by itself, but markedly increases the analog-induced membrane potential di~ipation and permeability. The data suggest that ATP-induced membrane permeation is composed of two processes: One is common to ATP and its analogs and appears to be a receptormediated process. The second is unique for ATP, effective even at low concentration (0.05 mM), and might be mediated by cell surface enzymes, for which ATP, but not its analogs, serves as a substrate.

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“…Complementary DNA (cDNA) synthesis from the equivalent quantity of the total RNA was done using the Transcriptor First Strand synthesis kit (Roche). StepOnePlus™ Real-Time PCR system (Applied Biosystems) and SYBR green master mix (Ampliqon) were used to analyze the relative expression level of P2RX 1 , P2RX 2 , 14 and PANX1 genes. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as the endogenous control.…”

Section: Analysis Of Gene Expression Using Real-time Quantitative Pmentioning

confidence: 99%

“…It affects intracellular calcium concentration in both human and murine macrophages. 13,14 It is demonstrated that alveolar macrophages have all subtypes of P2 receptors except P2Y 12 , P2X 2 , P2X 3 and P2X 6 .…”

mentioning

confidence: 99%

P2 receptors mRNA expression profiles in macrophages from ankylosing spondylitis patients and healthy individuals

et al. 2019

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Background Ankylosing spondylitis (AS) is a multifactorial rheumatic disease which mainly involves the axial skeleton. Macrophages and extracellular nucleotides have been shown to contribute to the inflammation process in autoimmune diseases. Membrane‐bound purinergic P2 receptors might be involved in the modulation of immune cells in AS. Therefore, we aimed to analyze the messenger RNA (mRNA) expression of P2 receptors in the macrophages of AS patients and healthy controls. Methods Twenty‐three AS patients and 23 age‐ and sex‐matched healthy individuals were included in our study. Whole blood‐separated monocytes of study participants were stimulated by macrophage colony‐stimulating factor for 7 days and differentiated to macrophages. Monocyte and macrophage markers were analyzed by flow cytometry. SYBR green real‐time polymerase chain reaction was used to measure the relative expression levels of P2RX1, P2RX2, P2RX3, P2RX4, P2RX5, P2RX6, P2RX7, P2RY1, P2RY2, P2RY4, P2RY6, P2RY11, P2RY12, P2RY13, P2RY14, and PANX1 genes. Results P2RY13 and P2RY6 genes had the highest expression levels in macrophages among P2RY genes. P2RY1 mRNA expression was significantly down‐regulated (−1.75 fold) and P2RY14 was up‐regulated (2.6 fold) in macrophages of AS patients compared to healthy individuals. P2RX4 gene had the highest expression in monocyte‐derived macrophages, followed by P2RX7 and P2RX1 genes. There was no significant difference in P2X receptor mRNA expression level between macrophages of AS patients and healthy individuals. Conclusions Our results indicate that AS patients show altered expression levels of P2 receptor genes. Moreover, these changes might be associated with disease activity and patients’ status.

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P2Z adenosine triphosphate receptor activity in cultured human monocyte- derived macrophages

Cited by 13 publications

References 0 publications

Ecto‐phosphodiesterase/pyrophosphatase of lymphocytes and non‐lymphoid cells: structure and function of the PC‐1 family

Ecto‐phosphodiesterase/pyrophosphatase of lymphocytes and non‐lymphoid cells: structure and function of the PC‐1 family

Combined effects of ATP and its analogs on the membrane permeability in transformed mouse fibroblasts

P2 receptors mRNA expression profiles in macrophages from ankylosing spondylitis patients and healthy individuals

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