P2Y purinoceptors induce changes in intracellular calcium in acinar cells of rat lacrimal glands

Kamada, Yuki; Saino, Tomoyuki; Oikawa, Makoto; Kurosaka, Daijiro; Satoh, Yuichi

doi:10.1007/s00418-011-0885-0

Cited by 13 publications

(22 citation statements)

References 47 publications

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“…Із цієї групи, в базальній мембрані ацинарних клітин сльозових залоз мишей, екс пресуються Р2Y 11 і Р2Y 13 -рецептори [36]. АТР-індуковані відповіді ацинарних клітин сльозових залоз щурів реалізуються (поряд з активацією P2X-рецепторів) переважно через активацію P2Y 1 -рецепторів і наступну ІР 3 -опосередковану мобілізацію Са 2+ , що пока-зано з використанням гепарину та інгібітору фосфоліпази С -U73122, а також зворотної транскриптазної полімеразної ланцюгової реакції [20].…”

unclassified

“…Також було ідентифіковано [20] (крім Р2Y 1 ) у секреторних клітинах сльозових за-лоз P2Y 2,4,12,14 , але не підтвердили експресії Р2Y 11 і Р2Y 13 -рецепторів. Питання про те, через які саме підтипи P2Y-рецепторів опосередковується механізм сигнальної трансдукції під впливом АТР є дуже важли-вим, адже механізм сигнальної трансдукції під час активації різних Р2Y-рецепторів може за-лучати різні сигнальні шляхи [8,37,38].…”

unclassified

“…Але відкидати наявність інших механізмів внутрішньоклітинної трансдукції АТР-індукованого сигналу ми не можемо, оскільки карбахолін-та ІР 3 -індуковане (але не АТР-індуковане) зменшення вмісту Са 2+ у досліджуваних клітинах повністю інгібується 2-АФб. Це також підтверджується тим, що інгібітор фосфоліпази C -U73122 (5 мкМ) лише частково інгібує АТР-індуковане збільшення цитозольної концентрації Са 2+ [20]. як за активації М-холінорецепторів, так і Р2Y-рецепторів механізм сигнальної трансдукції в секреторних клітинах сльозових залоз той самий.…”

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IP(3)-sensitive Ca(2+)-channels of endoplasmic reticulum in secretory cells of the rat exorbital lacrimal gland

Kotliarova¹,

Manko²

2013

Ukr.Biochem.J.

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IP(3)-sensitive Ca(2+)-channels of endoplasmic reticulum in secretory cells of the rat exorbital lacrimal gland

Kotliarova¹,

Manko²

2013

Ukr.Biochem.J.

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“…For this purpose, we examined the [Ca 2+ ] i dynamics in semi-intact rat acinar cells that retained their essential cellular structure. We recently showed that ATP and protease-activeted receptor 2 participate in a variety of signaling activities in lacrimal glands (40,49). The present study focused on identification of the types of adrenoceptors that are present in acinar cells and the receptors that mediate the effect of NA on [Ca 2+ ] i levels in these cells.…”

Section: +mentioning

confidence: 96%

“…We recently reported that Ca 2+ mobilization from intracellular Ca 2+ stores is induced by extracellular ATP, suggesting the presence of metabotropic receptors that are activated by ATP in rat lacrimal gland acinar cells (40). Purinergic receptors are important signaling molecules that are activated by ATP.…”

Section: Muc Mrna Expression In Lacrimal Gland Acinar Cellsmentioning

confidence: 99%

α1-Adrenoceptors relate Ca2+ modulation and protein secretions in rat lacrimal gland

et al. 2015

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Noradrenaline (NA) is a catecholamine with multiple roles including as a hormone and a neurotransmitter. Cellular secretory activities are enhanced by adrenergic stimuli as well as by cholinergic stimuli. The present study aimed to determine which adrenoceptors play a role in controlling intracellular calcium ion ([Ca 2+ ] i ) level in acinar cells of rat lacrimal glands. Expression of mRNA for adrenoceptor subtypes in the acinar cells was assessed using RT-PCR. ] i increase. The peroxidase activity was quantified as a measure of mucin secretion. Ca 2+ -dependent exocytotic secretion of peroxidase was detected in rat lacrimal glands. The RT-PCR results showed that MUC1, MUC4, MUC5AC, MUC5B, and MUC16 were expressed in acinar cells. These findings indicated that NA activates α1-adrenoceptors, which were found to be the main receptors in Ca 2+ -related cell homeostasis and protein (including mucin) secretion in lacrimal glands.The lacrimal gland is the main contributor to the aqueous component of the preocular tear film, which contains water, electrolytes, proteins, peroxidase, mucins, and lactoferrin. The lacrimal gland is composed of several cell types, including myoepithelial cells, ductal cells, goblet cells, and acinar cells, which are the major cell type constituting 80% of the gland. Acinar cells are highly polarized and joined by tight junctions at the luminal membrane, creating distinct basolateral and apical membranes (12). Goblet cells are a source of mucus in tears and secrete different types of mucins onto the ocular surface, especially in the conjunctiva. Although the number of goblet cells increases in the nasolacrimal duct, as the diameter of the nasolacrimal duct lumen is narrower than that of the lacrimal sac. Regulation of lacrimal gland fluid secretions is under neural control; activation of the sensory nerves in the cornea and conjunctiva initiates an afferent pathway leading to the central nervous system, which subsequently activates an efferent pathway that stimulates parasympathetic and sympathetic nerves, and ultimately, the lacrimal gland (32). The appropriate amount and composition of lacrimal gland fluid are crucial for a healthy, intact ocular surface (14). Sympathetic nerves release the catecholamine noradrenaline (NA). Catecholamines bind to adreno-

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Effects of protease-activated receptors (PARs) on intracellular calcium dynamics of acinar cells in rat lacrimal glands

Oikawa

Saino

Kimura

et al. 2013

Histochem Cell Biol

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Protease-activated receptors (PARs) represent a novel class of seven transmembrane domain G-protein coupled receptors, which are activated by proteolytic cleavage. PARs are present in a variety of cells and have been prominently implicated in the regulation of a number of vital functions. Here, lacrimal gland acinar cell responses to PAR activation were examined, with special reference to intracellular Ca(2+) concentration ([Ca(2+)]i) dynamics. In the present study, detection of acinar cell mRNA specific to known PAR subtypes was determined by reverse transcriptase polymerase chain reaction. Only PAR2 mRNA was detected in acinar cells of lacrimal glands. Both trypsin and a PAR2-activating peptide (PAR2-AP), SLIGRL-NH2, induced an increase in [Ca(2+)]i in acinar cells. The removal of extracellular Ca(2+) and the use of Ca(2+) channel blockers did not inhibit PAR2-AP-induced [Ca(2+)]i increases. Furthermore, U73122 and xestospongin C failed to inhibit PAR2-induced increases in [Ca(2+)]i. The origin of the calcium influx observed after activated PAR2-induced Ca(2+) release from intracellular Ca(2+) stores was also evaluated. The NO donor, GEA 3162, mimicked the effects of PAR2 in activating non-capacitative calcium entry (NCCE). However, both calyculin A (100 nM) and a low concentration of Gd(3+) (5 μM) did not completely block the PAR2-AP-induced increase in [Ca(2+)]i. These findings indicated that PAR2 activation resulted primarily in Ca(2+) mobilization from intracellular Ca(2+) stores and that PAR2-mediated [Ca(2+)]i changes were mainly independent of IP3. RT-PCR indicated that TRPC 1, 3 and 6, which play a role in CCE and NCCE, are expressed in acinar cells. We suggest that PAR2-AP differentially regulates both NCCE and CCE, predominantly NCCE. Finally, our results suggested that PAR2 may function as a key receptor in calcium-related cell homeostasis under pathophysiological conditions such as tissue injury or inflammation.

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P2Y purinoceptors induce changes in intracellular calcium in acinar cells of rat lacrimal glands

Cited by 13 publications

References 47 publications

IP(3)-sensitive Ca(2+)-channels of endoplasmic reticulum in secretory cells of the rat exorbital lacrimal gland

IP(3)-sensitive Ca(2+)-channels of endoplasmic reticulum in secretory cells of the rat exorbital lacrimal gland

<b>α1-Adrenoceptors relate Ca</b><sup><b>2+</b></sup><b> modulation and protein secretions in rat lacrimal </b><b>gland </b>

Effects of protease-activated receptors (PARs) on intracellular calcium dynamics of acinar cells in rat lacrimal glands

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