P2RX7 in Dopaminergic Neurons of Ventral Periaqueductal Gray Mediates HTWP Acupuncture-Induced Consciousness in Traumatic Brain Injury

Tang, Huiling; Qin, Siru; Li, Wei; Chen, Xuyi; Ulloa, Luis; Zhu, Qiumei; Liu, Baohu; Gong, Yi-Nan; Zhao, Yadan; Wang, Songtao; Li, Shanshan; Guo, Yongming; Xu, Zhifang; Guo, Yi

doi:10.3389/fncel.2020.598198

Cited by 10 publications

(9 citation statements)

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“…It is easy to develop into the second stage if not provided prompt and effective treatment, which causes a poor prognosis and low quality of life (QoL), bringing a huge economic burden to family and society. 4 Many studies 5 , 6 , 7 have proved that acupuncture was beneficial to TBI in brain function restoration and QoL improvement.…”

Section: Introductionmentioning

confidence: 99%

Acupuncture promotes nerve repair through the benign regulation of mTOR‐mediated neuronal autophagy in traumatic brain injury rats

et al. 2022

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Aims Recent investigations have already proved the neuroprotective efficacy of acupuncture in clinical practice in the treatment of neurological diseases, such as traumatic brain injury (TBI). Since growing evidence has suggested that neuronal autophagy was involved in multiple stages of TBI, this study aims to clarify the autophagy mediating mechanism underlying the neuroprotective effect of acupuncture in TBI rats. Methods Three experiments were carried out to detect changes in neuronal autophagy and identify the potential molecular mechanism underlying the neuroprotective effect of acupuncture for TBI treatment. Feeney's free‐falling epidural impingement method was used to establish the moderate TBI rat model; modified neurological severity scoring (mNSS) was used for neurological recovery evaluation. Nissl and HE staining were used to examine the histopathological changes. Immunofluorescence was used to detect the LC3‐positive cell rate. The transmission electron microscope (TEM) was used to investigate the morphology and quantity of autophagosomes. Western blotting was used to determine the protein expressions of LC3, p62, beclin1, mTOR, ULK1, p‐mTOR, and p‐ULK1. Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used for gene expressions analysis of LC3 mRNA and p62 mRNA. Co‐immunoprecipitation (CO‐IP) method was used to identify the protein interaction of mTOR and ULK1. Results On Day 3 after TBI, acupuncture accelerated the removal of damaged cellular structures by promoting neuronal autophagy; on Day 7 and Day 14 after TBI, acupuncture inhibited neuronal autophagy, preventing excessive autophagy and thus alleviated nerve damage. In addition, the simultaneous treatment with 3‐MA or rapamycin at different stages after TBI attenuated the effect of acupuncture. Conclusion Acupuncture has a benign regulatory effect on neuronal autophagy in different stages of TBI, possibly through the mTOR/ULK1 pathway.

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Section: Introductionmentioning

confidence: 99%

Acupuncture promotes nerve repair through the benign regulation of mTOR‐mediated neuronal autophagy in traumatic brain injury rats

et al. 2022

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“…Najem's study ( 43 ) indicated that acupuncture could reduce the efficacy of secondary brain injuries by reducing systemic and local inflammation, oxidative stress, intracellular calcium overload, neuronal regeneration, and growth factor release. Tang's research also revealed that acupuncture could activate genes modulating neuronal projections (P2rx7, P2rx3, Trpv1, Tacr1, and Cacna1d), protein secretion (Exoc1, Exoc3l1, Fgb, and Fgr), and dopamine (DA) receptor D3 (Drd3) in the ventral periaqueductal gray (vPAG), as well as the expression and excitability of DA and P2RX7 neurons ( 44 ). These may be the potential mechanisms of acupuncture for consciousness improvement in patients following stroke.…”

Section: Discussionmentioning

confidence: 99%

Effect of acupuncture for disorders of consciousness in patients with stroke: A systematic review and meta-analysis

et al. 2022

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BackgroundDisorder of consciousness (DOC) is frequent in patients with stroke, which is the second most common cause of death and a leading cause of disability. Acupuncture has been used as a curative method for DOC treatment in China. Nevertheless, no critical systematic review of acupuncture's effect on DOC has been published. This review aims to evaluate the present evidence regarding the efficacy of acupuncture for DOC after stroke.MethodsSeven databases were searched from their inception to November 1, 2021, containing three English databases (PubMed, Embase, and Cochrane Central Register of Controlled Trials) and four Chinese databases (CNKI, CBM, VIP, and Wanfang Database). The primary outcomes comprise the Glasgow Coma Scale (GCS) and Glasgow Outcome Scale (GOS) before and after treatment. Secondary outcomes involve resuscitation rate, resuscitation time, and adverse events. Data synthesis was calculated by RevMan (V.5.4.1) software. According to the Cochrane Handbook, methodological quality was assessed with the risk of bias tool 2.0 (RoB2).ResultsSeventeen studies containing 1,208 patients were eventually included in our review. Overall, most trials were rated as high or had some concerns regarding the risk of bias. GCS was reported in 16 trials, and a meta-analysis showed that GCS improvement in the acupuncture group was greater than in the non-acupuncture group (MD 1.45, 95% CI 0.94–1.97, P < 0.0001). One trial reported that GOS improvement in the acupuncture plus medication group was greater than in the medication group (MD 0.58, 95% CI 0.11–1.05, P = 0.01). Another study reported that acupuncture plus medication was statistically more effective in shortening resuscitation time than medication alone (MD−0.89, 95% CI −1.53 to −0.25, P = 0.006). Four trials reported that the resuscitation rate in the acupuncture group was higher than without acupuncture intervention (RR 1.68, 95% CI 1.30–2.18, I2 0%, P = 0.39). Adverse events were reported in two studies, with one case in the acupuncture group suffering from subcutaneous hematoma.ConclusionAcupuncture may improve consciousness level, increase the resuscitation rate, and shorten resuscitation time for post-stroke patients with DOC. Adverse events from acupuncture were rare, tolerable, and recoverable. However, the results should be interpreted cautiously, and more rigorous RCTs with better methodology are warranted.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/display_record.php?RecordID=289802, identifier 289802.

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“…Exocyst complex component 3 like 1 (EXOC3L1), also known as EXOC3L, is localized on chromosome 16 (16q22.1). Up to the present day, limited studies have shown that EXOC3L1 were involved in the regulation of insulin secretion ( Saito et al, 2008 ), protein secretion after traumatic brain injury ( Tang et al, 2020 ), spontaneous induction of apoptosis ( Matsumoto et al, 2021 ), and high-density lipoprotein concentration ( Lanktree et al, 2015 ), but the exact function of EXOC3L1 remains unclear. There are no reports on the study of EXOC3L1 in tumors so far, and the roles of EXOC3L1 in pan-cancer are far from clear, making it necessary to explore the role of EXOC3L1 in pan-cancer.…”

Section: Introductionmentioning

confidence: 99%

A comprehensive prognostic and immune infiltration analysis of EXOC3L1 in pan-cancer

Zhang

2022

Front. Genet.

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Exocyst complex component 3 like 1 (EXOC3L1) is widely present in various human tissues, which mainly regulates insulin secretion. However, its roles in tumors remain unclear. In the present study, we aimed to investigate the roles of EXOC3L1 in pan-cancer, and the data was downloaded from of the University of California Santa Cruz (UCSC) Xena and the Cancer Genome Atlas (TCGA). The expression status of EXOC3L1 was studied in the TCGA_GTEx samples, TCGA samples and paired samples in TCGA, respectively. Subsequently, Kaplan-Meier analysis was applied to 33 kinds of tumors in TCGA, among the cancers that EXOC3L1 can affect prognosis, clinical correlation analysis and univariate Cox regression analysis were performed. Furthermore, representative cancers kidney renal clear cell carcinoma (KIRC) and lung squamous cell carcinoma (LUSC) with a sample size larger than 500 were selected to construct nomogram models to confirm the prognostic value of EXOC3L1 in cancers. Additionally, the associations of EXOC3L1 with immune cell infiltrations were performed as well. Mechanistically, functional enrichment analysis was performed to explore potential signaling pathways that EXOC3L1 may involve in. Our study found that EXOC3L1 was differentially expressed in a variety of tumors and was associated with the clinical outcomes and immune microenvironment of several tumors, it may affect the occurrence and development of tumors through NOTCH signaling pathway, PI3K-AKT signaling pathway and immune-related pathways. In conclusion, we propose that EXOC3L1 may serve as a potential prognostic biomarker and a promising target for cancer immunotherapy in a variety of cancers.

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P2RX7 in Dopaminergic Neurons of Ventral Periaqueductal Gray Mediates HTWP Acupuncture-Induced Consciousness in Traumatic Brain Injury

Cited by 10 publications

References 56 publications

Acupuncture promotes nerve repair through the benign regulation of mTOR‐mediated neuronal autophagy in traumatic brain injury rats

Acupuncture promotes nerve repair through the benign regulation of mTOR‐mediated neuronal autophagy in traumatic brain injury rats

Effect of acupuncture for disorders of consciousness in patients with stroke: A systematic review and meta-analysis

A comprehensive prognostic and immune infiltration analysis of EXOC3L1 in pan-cancer

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