p27&lt;SUP&gt;kip1&lt;/SUP&gt; Expression Is Inversely Related to the Grade of Gastric MALT Lymphoma

Shirin, Haim; Kravtsov, Vladimir; Shahmurov, Mark; Petchenko, Paulina; Boaz, Mona; Moss, Steven F.; Avni, Yona; Avinoach, Ilana

doi:10.1385/ijgc:35:1:025

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Our results confirm a strong correlation between the presence of H. pylori in gastric adenocarcinoma and a lack of p27 expression as 37 out of 42 samples with positive ureA were negative for p27 expression with a significant association (p = 0.001). This result is consistent with a study done in vitro by Shirin et al, who found a strong correlation between the presence of H. pylori and the inhibition of p27 immunoexpression [46]. Although prior studies have indeed found a correlation between different carcinoma types and expression of p27, we believe this is the first study to document this using validated viable gastrectomy samples.…”

Section: Discussionsupporting

confidence: 92%

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

Brown

Mohammad

Shboul

et al. 2022

Preprint

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Gastric cancer is one of the most prevalent malignant cancers worldwide and specifically, adenocarcinoma. Based on prior research, there is an association between Helicobacter pylori (H. pylori) infection and the frequency of duodenal ulcer, distal gastric adenocarcinoma, mucosa-associated lymphoid tissue lymphoma, and antral gastritis. H. pylori virulence and toxicity factors have been identified that significantly influence the clinical outcomes of H. pylori infection and gastric adenocarcinoma. However, it is unclear exactly how different strains of H. pylori infection affect gastric adenocarcinoma. Current research suggests this involves tumor suppressor genes, like p27, but also H. Pylori toxic proteins. Therefore, we quantified known H. Pylori genotypes within adenocarcinoma patients to establish the prevalence of known toxins that include cytotoxin-associated gene A (cagA) and vacuolating cytotoxin A (vacA) within patients of variable diagnosis. This analysis used gastrectomy samples which were validated for DNA viability. The incidence of H. Pylori in adenocarcinoma patients in Jordan was established to be 54.5% positive (ureA gene) with cagA genotype occurrence at 57.1% but also vacA gene ratios 24.7%:22.1%: 14.3%:14.3%. (vacAs1:vacAs2: vacAm1:vacAm2). It is statistically significant that p27 was dysregulated and suppressed within nearly all H. Pylori vacA genotypes but also that according to our analysis that 24.6% of H. Pylori samples analyzed had an unknown novel genotype and curiously that p27 protein expression was retained in only 12% of tested adenocarcinoma H. Pylori samples which is suggestive that p27 could be used as a prognostic indicator but also that an un-known yet genotype as yet not characterized could be contributing to the regulation of p27 protein within this cellular environment.

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Section: Discussionsupporting

confidence: 92%

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

Brown

Mohammad

Shboul

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Our results confirm a strong correlation between the presence of H. pylori in gastric adenocarcinoma and a lack of p27 expression as 37 out of 42 samples with positive ureA were negative for p27 expression with a significant association (p = 0.001). This result is consistent with a study done in vitro by Shirin et al, who found a strong correlation between the presence of H. pylori and the inhibition of p27 immunoexpression [63]. Although prior studies have found a correlation between different carcinoma types and expression of p27, we believe this is the first study to document this using validated viable gastrectomy samples.…”

Section: Discussionsupporting

confidence: 92%

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

Al-Sheboul

Mohammad

Shboul

et al. 2023

J Epidemiol Glob Health

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Stomach (gastric) cancer is one of the most prevalent and deadly cancers worldwide and most gastric cancers are adenocarcinomas. Based on prior research, there is an association between Helicobacter pylori (H. pylori) infection together with the frequency of duodenal ulcer, distal gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and antral gastritis. Helicobacter pylori virulence and toxicity factors have been identified before that significantly influence the clinical outcomes of H. pylori infection and gastric adenocarcinoma. However, it remains unclear exactly how different strains of H. pylori affect gastric adenocarcinoma. Current research suggests this involves tumor suppressor genes, like p27 but also H. pylori toxic virulence proteins. Therefore, we quantified known H. pylori genotypes within adenocarcinoma patients to establish the prevalence of known toxins that include cytotoxin-associated gene A (cagA) as well as vacuolating cytotoxin A (vacA) within patients of variable adenocarcinoma diagnosis. This analysis used gastrectomy samples validated for DNA viability. The incidence of H. pylori in adenocarcinoma patients in Jordan was established to be 54.5% positive (ureA gene positive) with cagA genotype occurrence at 57.1%, but also in this population study vacA gene ratios found to be 24.7%:22.1%:14.3%:14.3%. (vacAs1:vacAs2:vacAm1:vacAm2). Using immunohistochemistry (IHC), we confirmed with statistical significance that p27 was dysregulated and suppressed, within nearly all H. pylori vacA genotypes. In addition, within 24.6% of H. pylori samples analyzed was a different bacterial genotype, and curiously that p27 protein expression was retained in 12% of tested adenocarcinoma H. pylori samples. This is suggestive that p27 could be used as a prognostic indicator but also that an unknown genotype could be contributing to the regulatory effects of p27 protein within this bacterial and cellular environment that may include other virulence factors and unknown immune system regulatory changes.

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“…Kip1 expression in gastric MALT lymphomas and DLBCLs correlated with the grade of the lymphoma, irrespective of the stage 13 . While p27 Kip1 was not detectable in either the large cell or MALT components of DLBCLs with MALT lymphoma, p27…”

Section: Discussionmentioning

confidence: 88%

p27Kip1 is Detected on Most Gastric MALT Lymphomas, but not Large Cell Lymphomas

Sato

Ichimura

et al. 2006

J Clin Exp Hematopathol

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We investigated the relationship of gastric mucosa-associated lymphoid tissue (MALT) lymphoma tumorigenesis to Helicobacter pylori infection, the t (11 ; 18) translocation, and alterations in cell cycle regulators. We sought to assess the implications of altered expression of p27 Kip1 , a cyclin-dependent kinase inhibitor, on high-grade transformation and responsiveness to eradication therapy. We used immunohistochemistry to examine p27Kip1 , p53, and Ki-67 expression in 23 MALT lymphomas, five diffuse large B-cell lymphomas (DLBCLs), and four DLBCLs with associated MALT lymphoma. All of the MALT lymphomas were positive for p27Kip1 expression and negative for p53 with a low Ki-67 index, regardless of the sensitivity of these cells to eradication. All DLBCLs were negative for p27 Kip1 and positive for p53, exhibiting a high Ki-67 index. In DLBCLs with MALT lymphoma, p27Kip1 expression was absent from both the MALT and large cells components. In all of these lymphomas, the MALT components were negative for p53 and displayed a low Ki-67 index, while the large cell components were positive for p53 with a high Ki-67 index. The expression patterns of the DLBCLs differed significantly from those of the MALT lymphomas. p27Kip1 was not detected in either component of DLBCL with MALT lymphoma, suggesting that decreased expression of p27Kip1 in the MALT component may be related to high-grade transformation. Thus, p27Kip1 expression in morphological MALT lymphomas could be useful tool to predict high-grade transformation to DLBCL.

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p27<SUP>kip1</SUP> Expression Is Inversely Related to the Grade of Gastric MALT Lymphoma

Abstract: p27kip1 may be a putative distinct molecular marker to differentiate between low-grade and high-grade gastric lymphoma.

Cited by 3 publications

References 27 publications

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

A Genetic and Immunohistochemical Analysis ofH. PyloriPhenotypes and p27 expression in Adenocarcinoma Patients in Jordan

A Genetic and Immunohistochemical Analysis of Helicobacter pylori Phenotypes and p27 Expression in Adenocarcinoma Patients in Jordan

p27Kip1 is Detected on Most Gastric MALT Lymphomas, but not Large Cell Lymphomas

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