p27 expression and gastric carcinoma

Mori, Masaki; Mimori, Koshi; Shiraishi, Takeshi; Tanaka, Shinji; Ueo, Hiroaki; Sugimachi, Keizō; Akiyoshi, Tsuyoshi

doi:10.1038/nm0697-593

Cited by 245 publications

(186 citation statements)

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“…Alterations in p27 Kip1 warrant particular attention as p27 Kip1 protein levels are reduced in several types of cancer including melanoma (Catzavelos et al, 1997;Loda et al, 1997;Mori et al, 1997;Florenes et al, 1998;Kawana et al, 1998), and p27 Kip1 heterozygous mice are prone to induced tumor formation (Fero et al, 1998). B-RAF is mutated in approximately 70% of melanomas (Davies et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells

Bhatt

Spofford

et al. 2006

Oncogene

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Section: Discussionmentioning

confidence: 99%

Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells

Bhatt

Spofford

et al. 2006

Oncogene

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“…IAP is a well-established marker of di erentiation in HT29 cells and other colon cancer cell lines (Choi et al, 1990;Hodin et al, 1996, Schroy et al, 1994Goldberg et al, 1996;Navarro et al, 1997;Basson et al, 1996). IAP activity was examined using a histochemical demonstration kit for alkaline phosphatase detection (Sigma) (Goldberg et al, 1996) and also by Western blot analysis using a rabbit polyclonal antibody that recognizes human intestinal alkaline phosphatase (Dako), as described previously (Goldberg et al, 1996).…”

Section: E Ect Of P27 Kip1 Overexpression On Cell DI Erentiationmentioning

confidence: 99%

“…Sodium butyrate (SB) was used for induction of di erentiation. This short chain fatty acid is a di erentiating agent for various cell types (Navarro et al, 1997;Siavoshian et al, 1997;Basson et al, 1996;Conway et al, 1995;Swarovsky et al, 1994). By histochemical analyses IAP positive cells were only rarely detected in the untreated vector control and the overexpressor derivatives.…”

Section: E Ect Of P27 Kip1 Overexpression On Cell DI Erentiationmentioning

confidence: 99%

“…Indeed, we have found relatively high levels of expression of this gene in several colon, esophageal and breast cancer cell lines and in a subset of primary colon and breast carcinomas Sgambato et al, 1997;Ciaparrone et al, 1998). At the same time, several recent studies indicate that absent or low levels of expression of p27 in a subset of cancers of the colon, breast, lung, esophagus, stomach and prostate are associated with cases that have a poor prognosis Fredersdorf et al, 1997;Catzavelos et al, 1997;Porter et al, 1997;Esposito and Baldi et al, 1997;Mori et al, 1997;Singh et al, 1998;Yang et al, 1988). It is of interest that the expression of p27 is regulated mainly at the post-translational level via a ubiquitin-proteasome mediated proteolysis mechanism and the decreased levels in a subset of cancers appear to be due to enhanced activity of this proteolytic system (Pagano et al, 1995;Loda et al, 1997;Esposito and Baldi, 1997).…”

Section: Introductionmentioning

confidence: 99%

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Comparative effects of overexpression of p27Kip1 and p21Cip1/Waf1 on growth and differentiation in human colon carcinoma cells

et al. 1999

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Recent studies have shown that decreased expression of p27 Kip1 is associated with high grade tumors and an unfavorable prognosis in several types of human cancer. To clarify the role of p27 Kip1 in colon cancer, we have overexpressed this protein in the HT29 colon cancer cell line. The derivatives displayed an increase in the p27 Kip1 protein in cyclin E/CDK2 immunoprecipitates and a decrease in cyclin E-associated kinase activity when compared to vector control clones, providing evidence that the overexpressed protein was functional. Clones with a high level of p27 Kip1 displayed partial growth inhibition in monolayer culture and a decrease in plating e ciency, even though they expressed increased levels of the cyclin D1 protein. Using alkaline phosphatase expression as a marker, we found that the p27 Kip1 overexpressor clones displayed a 2 ± 3-fold increase in sensitivity to induction of di erentiation by 2 mM sodium butyrate. In contrast to these results, derivatives of HT29 cells that stably overexpressed p21 Cip1/Waf1 displayed decreased sensitivity to the induction of di erentiation. These ®ndings may explain why decreased levels of p27 Kip1 in certain human cancers is associated with high grade (poorly di erentiated) tumors, and suggest that strategies that increase the level of p27 Kip1 may be useful in cancer therapy.

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“…p27 Kip1 is present in large amounts in quiescent cells but has been found to be decreased in malignant tumors. Furthermore, a low expression of p27 Kip1 protein has been found to be associated with tumor aggressiveness and an unfavorable prognosis in several cancers, including those of the urinary bladder (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16). In transitional cell carcinoma of the upper urinary tract (TCC-UUT), however, the expression of p27 Kip1 protein has not been examined for its clinical significance in predicting survival.…”

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confidence: 99%

Expression of p27Kip1 Protein in Transitional Cell Carcinoma of the Upper Urinary Tract

et al. 2001

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The expression of p27 Kip1 , a negative regulator of the cell cycle, has been reported to correlate with the biological behavior and prognosis of several tumors. However, its prognostic importance in transitional cell carcinoma of the upper urinary tract (TCC-UUT) has not previously been investigated. We investigated p27 Kip1 protein expression using immunohistochemistry in 132 cases of TCC-UUT and also its relation to proliferating cell nuclear antigen (PCNA) immunoreactivity, p53 oncoprotein immunoreactivity, clinicopathologic parameters, and clinical outcome. A positive expression of p27 Kip1 protein was recognized in 94.7% of the samples and was apparent within tumor nuclei. In the normal urothelium, its expression was identified in all cell layers. A positive expression of p53 oncoprotein was recognized in 27.2% of the patients. The PCNA index was 7.4 to 93.1% (mean, 66.4%). Examination of the relationships between the expression of p27 Kip1 protein and clinicopathologic findings, PCNA index, and the expression of p53 oncoprotein revealed that the expression of p27 Kip1 protein decreased significantly with stage and grade. In a univariate analysis of disease-free and overall survival rates, no correlation was found between the expression of p27 Kip1 protein and prognosis. The expression of p27 Kip1 protein appears to be of little or no value in informing the prognosis in TCC-UUT.

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p27 expression and gastric carcinoma

Cited by 245 publications

References 4 publications

Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells

Mutant B-RAF signaling and cyclin D1 regulate Cks1/S-phase kinase-associated protein 2-mediated degradation of p27Kip1 in human melanoma cells

Comparative effects of overexpression of p27Kip1 and p21Cip1/Waf1 on growth and differentiation in human colon carcinoma cells

Expression of p27Kip1 Protein in Transitional Cell Carcinoma of the Upper Urinary Tract

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