p21WAF1/CIP1 protein expression in primary ovarian cancer.

Ferrandina, Gabriella; Stoler, Andrea; Fagotti, Anna; Fanfani, Francesco; Sacco, Rosario; Pasqua, Alessandro; Mancuso, Stefano

doi:10.3892/ijo.17.6.1231

Cited by 33 publications

(32 citation statements)

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“…The role of senescence as a determinant of chemotherapy outcome is poorly documented, perhaps because it has been overshadowed by the high interest in apoptosis. It has been reported that expression of the senescence-associated protein p21/ WAF1 correlates with improved survival after chemotherapy treatment in pancreatic cancer (Ahrendt et al, 2000), esophageal carcinoma (Nakashima et al, 2000), head and neck (Kapranos et al, 2001) and ovarian cancer (Ferrandina et al, 2000). In addition, recent findings suggest that senescence may play a key role in chemotherapy outcome Schmitt et al, 2002;te Poele et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Caspase inhibition switches doxorubicin-induced apoptosis to senescence

et al. 2003

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Section: Introductionmentioning

confidence: 99%

Caspase inhibition switches doxorubicin-induced apoptosis to senescence

et al. 2003

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“…In the group of stage III-IV ovarian cancer patients, p21-positive cases showed a more favorable prognosis than p21-negative cases: the 3-year time to progression rate was 58% for p21-positive cases and 33% for p21-negative cases (P ϭ 0.036; ref. 52). …”

Section: G 1 -S Regulatorsmentioning

confidence: 99%

Cell Cycle Genes in Ovarian Cancer

D’Andrilli

Kumar

Scambia

et al. 2004

Clinical Cancer Research

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Human malignant tumors are characterized by abnormal proliferation resulting from alterations in cell cycleregulatory mechanisms. The regulatory pathways controlling cell cycle phases include several oncogenes and tumor suppressor genes that display a range of abnormalities with potential usefulness as markers of evolution or treatment response in ovarian cancer. This review summarizes the current knowledge about these aberrations in malignant tumors of the ovary. We sought to divide cell cycle-regulatory genes into four subgroups on the basis of their predominant role in a specific phase or during the transition between two phases of the cell cycle.

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“…The vast majority of ovarian tumors arise from the malignant transformation of ovarian surface epithelium, which displays a variety of Mu¨llerian-type cells, including serous, mucinous, endometrioid and clear cell, with a common pathway in embryologic development. Ovarian cancer develops in a multistep process that involves alterations of multiple pathways, including p16 INK4A , p21 Waf1/Cip1 , p27, cyclin D1, p53 and pRb (Kusume et al, 1999;Ferrandina et al, 2000;Prowse et al, 2003;Ozols et al, 2004;Kauff et al, 2005;Rosen et al, 2005). Our understanding of ovarian cancer is hampered by lack of human cell model system for studying early neoplastic changes (Vasey, 2003;Tammela and Odunsi, 2004).…”

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confidence: 99%

Disruption of the retinoblastoma pathway by small interfering RNA and ectopic expression of the catalytic subunit of telomerase lead to immortalization of human ovarian surface epithelial cells

et al. 2006

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The risk of developing ovarian cancer is about 1% over a lifetime, but it is the most deadly gynecologic cancer, in part due to lack of diagnostic markers for early-stage disease and cell model system for studying early neoplastic changes. Most existing immortal human ovarian surface epithelial cells were achieved by using viral protein such as SV40 T/t antigen or E6/E7, which inactivate multiple cellular pathways. In the current study, we used a small interfering RNA (siRNA) against the retinoblastoma gene (pRb) and ectopic expression of human telomerase reverse transcriptase (hTERT) to immortalize the primary ovarian epithelial cell line OSE137 and two additional human ovarian surface epithelial cells. The immortalized OSE137 showed increased telomerase activity, lengthened telomeres, increased G 2 /M phase, altered cell-cycle regulatory proteins but nontumorigenic. As both Rb and hTERT pathways are commonly altered in human ovarian cancer and these genetic changes are faithfully modeled in these cells without using viral protein, these immortal cells represent an authentic in vitro model system with which to study the initiation and progression of human ovarian cancer.

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p21WAF1/CIP1 protein expression in primary ovarian cancer.

Cited by 33 publications

References 0 publications

Caspase inhibition switches doxorubicin-induced apoptosis to senescence

Caspase inhibition switches doxorubicin-induced apoptosis to senescence

Cell Cycle Genes in Ovarian Cancer

Disruption of the retinoblastoma pathway by small interfering RNA and ectopic expression of the catalytic subunit of telomerase lead to immortalization of human ovarian surface epithelial cells

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