P2 receptors in the murine gastrointestinal tract

Giaroni, Cristina; Knight, Gillian E.; Ruan, Huai Zhen; Glaß, Rainer; Bardini, Michelle; Lecchini, Sergio; Frigo, Gianmario; Burnstock, Geoffrey

doi:10.1016/s0028-3908(02)00294-0

Cited by 106 publications

(130 citation statements)

References 36 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…These slow EPSPs only occurred in NOS-positive descending interneurons [103]. These data are supported by work in the mouse demonstrating that P2Y 1 receptors are on NOSpositive myenteric neurons [74].…”

Section: Slow Synaptic Transmissionsupporting

confidence: 72%

“…Ninety percent of the intrinsic sensory neurons stained for the P2X 2 receptor, with one-third of these staining strongly and the rest staining weakly. In the mouse intestine, P2X 2 receptor mRNA has been localized to a much smaller population of myenteric neurons of unknown functional class [74]. In the guinea pig, P2X 3 receptors are found on a variety of myenteric neurons, including some NOS-positive neurons (inhibitory motor and descending interneurons) and ascending interneurons or longitudinal muscle motor neurons, but not on sensory neurons [75,76].…”

Section: Localization Of P2x Receptors In the Ensmentioning

confidence: 99%

“…Work in the mouse myenteric plexus has shown that immunoreactivity for P2Y 1 receptors is located on NOS immunoreactive neurons (descending interneurons or inhibitory motor neurons) and that mRNA can be found in both myenteric and submucous plexes [74]. Work in the submucous plexus has suggested that neurons expressing P2Y 1 receptors are secretomotor [20,96].…”

Section: Localization Of P2y Receptors In the Ensmentioning

confidence: 99%

See 2 more Smart Citations

Purinergic receptors and synaptic transmission in enteric neurons

Ren

Bertrand

2007

Purinergic Signalling

View full text Add to dashboard Cite

Purines such as ATP and adenosine participate in synaptic transmission in the enteric nervous system as neurotransmitters or neuromodulators. Purinergic receptors are localized on the cell bodies or nerve terminals of different functional classes of enteric neurons and, with other receptors, form unique receptor complements. Activation of purinergic receptors can regulate neuronal activity by depolarization, by regulating intracellular calcium, or by modulating second messenger pathways. Purinergic signaling between enteric neurons plays an important role in regulating specific enteric reflexes and overall gastrointestinal function. In the present article, we review evidence for purine receptors in the enteric nervous system, including P1 (adenosine) receptors and P2 (ATP) receptors. We will explore the role they play in mediating fast and slow synaptic transmission and in presynaptic inhibition of transmission. Finally, we will examine the molecular properties of the native receptors, their signaling mechanisms, and their role in gastrointestinal pathology. Keywords

show abstract

Section: Slow Synaptic Transmissionsupporting

confidence: 72%

Section: Localization Of P2x Receptors In the Ensmentioning

confidence: 99%

Section: Localization Of P2y Receptors In the Ensmentioning

confidence: 99%

See 1 more Smart Citation

Purinergic receptors and synaptic transmission in enteric neurons

Ren

Bertrand

2007

Purinergic Signalling

View full text Add to dashboard Cite

show abstract

“…In human and mouse intestine, IJPs and the associated relaxations are blocked by the P2Y 1 receptor antagonist MRS 2179 [92,98]. Furthermore, immunoreactivity for the P2Y 1 receptor protein is located on the smooth muscle in both species [92,115]. Together with the knowledge that enteric nerve stimulation releases ATP and its metabolites (see above), these data are compelling evidence that ATP acting at P2Y 1 receptors mediates IJPs evoked by activity in intrinsic inhibitory motor neurons.…”

Section: Inhibitory Neuromuscular Transmissionmentioning

confidence: 99%

“…Intestinal smooth muscle is often excited by P2X receptor activation [115], so receptors on ICC may be important. Furthermore, some ICCs are pacemakers for the smooth muscle, driving slow waves that set the underlying rhythm for motor activity (for review see [141]).…”

Section: P2x Receptors Within the Musclementioning

confidence: 99%

Purinergic mechanisms in the control of gastrointestinal motility

Bornstein

2007

Purinergic Signalling

View full text Add to dashboard Cite

For many years, ATP and adenosine have been implicated in movement regulation of the gastrointestinal tract. They act through three major receptor subtypes: adenosine or P1 receptors, P2X receptors and P2Y receptors. Each of these major receptor types can be subdivided into several different classes and is widely distributed amongst various neurons, muscle types, glia and interstitial cells that regulate intestinal functions. Several key roles for the different receptors and their endogenous ligands have been identified in physiological and pharmacological studies. For example, adenosine acting at A 1 receptors appears to inhibit intestinal motility in various pathological conditions. Similarly, ATP acting at P2Y receptors is an important component of inhibitory neuromuscular transmission, acting as a cotransmitter with nitric oxide. ATP acting at P2X and P2Y 1 receptors is important for synaptic transmission in simple descending excitatory and inhibitory reflex pathways. Some P2Y receptor subtypes prefer uridine nucleotides over purine nucleotides. Thus, roles for UTP and UDP as enteric transmitters in place of ATP cannot be excluded. ATP also appears to be important for sensory transduction, especially in chemosensitive pathways that initiate local inhibitory reflexes. Despite this evidence, data are lacking about the roles of either adenosine or ATP in more complex motility patterns such as segmentation or the interdigestive migrating motor complex. Clarification of roles for purinergic transmission in these common, but understudied, motility patterns will depend on the use of subtype-specific antagonists that in some cases have not yet been developed.

show abstract

Morphological Characterization of the Myenteric Plexus of the Ileum and Distal colon of Dogs Affected by Muscular Dystrophy

Schäfer

Silveira

Palombit

et al. 2017

The Anatomical Record

View full text Add to dashboard Cite

Duchenne-like muscular dystrophy (canine dystrophinopathy) is a hereditary degenerative disease characterized by muscle changes similar to those described for Duchenne muscular dystrophy (DMD) and by alterations in the smooth muscles of the gastrointestinal tract. Some authors have suggested that these abnormalities may be associated with intestinal motility. This study analyzed the nitrergic and cholinergic neurons and P2X7 receptor expression in the myenteric plexus of the ileum and distal colon of dogs with muscular dystrophy. Immunohistochemical techniques were used to detect nitric oxide synthase (NOS) and acetylcholine transferase (ChAT) expression and to label all HuC/D- and P2X7 receptor-immunoreactive (IR) neurons. Transmission electron microscopy and basic histology were performed for further analysis. The results showed that nitrergic neurons exhibited a Dogiel type I morphology in the ileum and distal colon. The neuronal profile results showed that there were fewer NOS-, ChAT-, and HuC/D-IR neurons in the ileum than in the distal colon in the dystrophic (DT) dogs. Additionally, there were more NOS-, ChAT- and HuC/D-IR neurons per ganglion in the distal colon than in the ileum. The P2X7 receptor-expressing neurons colocalized with nitrergic and cholinergic neurons. Transmission and light microscopy revealed collagen between the muscle fibers, between the circular and longitudinal muscle layers and within the myenteric ganglia of dogs with muscular dystrophy. These findings provide a morphological description of the myenteric neurons in the ileum and distal colon of these DT dogs and may contribute to a better understanding of the gastrointestinal disorders found in patients with DMD. Anat Rec, 301:673-685, 2018. © 2017 Wiley Periodicals, Inc.

show abstract

P2 receptors in the murine gastrointestinal tract

Cited by 106 publications

References 36 publications

Purinergic receptors and synaptic transmission in enteric neurons

Purinergic receptors and synaptic transmission in enteric neurons

Purinergic mechanisms in the control of gastrointestinal motility

Morphological Characterization of the Myenteric Plexus of the Ileum and Distal colon of Dogs Affected by Muscular Dystrophy

Contact Info

Product

Resources

About