P2.03-33 ctDNA Levels Significantly Predicts Survival in NSCLC Patients with an EGFR Activating Mutation

A label-free fluorescent sensor for rapid and sensitive detection of ctDNA based on fluorescent PDA nanoparticles

“…To investigate the specificity of this biosensor, we compared the luminescence response induced by mismatched base DNA (19 (2)) and wild type DNA (19 wt). Experiments were performed using a probe concentration of 250 nM, a target concentration of 50 nM, and a 10-fold target concentration of the interferer.…”

“…1 ctDNA is DNA released from cancer cells into body fluids, and some studies have shown ctDNA to be a noninvasive real-time biomarker for non-small cell lung cancer (NSCLC). [2][3][4] Amongst others, exon 19 deletion (ex19 del) ctDNA accounts for 60% of EGFR-mutant NSCLCs. The current conventional methods for ctDNA detection are targeted next generation sequencing (NGS), real-time quantitative polymerase chain reaction (qPCR), beads, emulsion, amplification, and magnetics (BEAMing), etc.…”