P1731 HBVDNA extraction from serum using the NucliSENS easyMAG platform

Deursen, P. van; Onland, G.; Brule, A. van den; Verhoeven, Adrie J.M.; Jacobs, Matthew

doi:10.1016/s0924-8579(07)71570-4

Cited by 1 publication

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“…Hereafter, 140 μL magnetic silica was added to each tube and thoroughly mixed with the sample. The remaining steps of the DNA extraction process were performed by the robot according to protocol A and eluted in 110 μL elution buffer (provided by easyMag ® ) [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

Microbial diversity in fecal samples depends on DNA extraction method: easyMag DNA extraction compared to QIAamp DNA stool mini kit extraction

et al. 2014

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BackgroundThere are challenges, when extracting bacterial DNA from specimens for molecular diagnostics, since fecal samples also contain DNA from human cells and many different substances derived from food, cell residues and medication that can inhibit downstream PCR. The purpose of the study was to evaluate two different DNA extraction methods in order to choose the most efficient method for studying intestinal bacterial diversity using Denaturing Gradient Gel Electrophoresis (DGGE).FindingsIn this study, a semi-automatic DNA extraction system (easyMag®, BioMérieux, Marcy I’Etoile, France) and a manual one (QIAamp DNA Stool Mini Kit, Qiagen, Hilden, Germany) were tested on stool samples collected from 3 patients with Inflammatory Bowel disease (IBD) and 5 healthy individuals.DNA extracts obtained by the QIAamp DNA Stool Mini Kit yield a higher amount of DNA compared to DNA extracts obtained by easyMag® from the same fecal samples. Furthermore, DNA extracts obtained using easyMag® seemed to contain inhibitory compounds, since in order to perform a successful PCR-analysis, the sample should be diluted at least 10 times. DGGE performed on PCR from DNA extracted by QIAamp DNA Stool Mini Kit DNA was very successful.ConclusionQIAamp DNA Stool Mini Kit DNA extracts are optimal for DGGE runs and this extraction method yields a higher amount of DNA compared to easyMag®.

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Section: Resultsmentioning

confidence: 99%