P152 Clinical and molecular epidemiology of ESBL producing Klebsiella pneumoniae and Escherichia coli in a tertiary hospital

Harada, Yoko; Yanagihara, K.; Kaku, Norihito; Migiyama, Yohei; Nagaoka, Kozo; Yamada, Kaoru; Morinaga, Y.; Hasegawa, Hiroo; Izumikawa, K.; Kakeya, Hiroshi; Kohno, S

doi:10.1016/s0924-8579(13)70396-0

Cited by 6 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies suggested that urine can be an important source of ESBLs-producing E. coli ( 27 , 28 ). To our knowledge, this the first report of ESBLs detection in E. coli isolates from UTIs in Libya using PCR.…”

Section: Discussionmentioning

confidence: 99%

Multidrug resistance and extended-spectrum β-lactamases genes amongEscherichia colifrom patients with urinary tract infections in Northwestern Libya

Abujnah

Zorgani

Sabri

et al. 2015

Libyan Journal of Medicine

View full text Add to dashboard Cite

IntroductionMultidrug resistance (MDR) and emergence of extended-spectrum β-lactamases (ESBLs) that mediate resistance to β-lactam drugs among Escherichia coli and other uropathogens have been reported worldwide. However, there is little information on the detection of ESBLs genes in E. coli from patients with urinary tract infections (UTIs) in the Arab countries using polymerase chain reaction (PCR), and in Libya such information is lacking.MethodsAll patients attending Zawiya Teaching Hospital in Zawiya city between November 2012 and June 2013 suspected of having UTIs and from whom midstream urine samples were taken as part of the clinical workup were included in this prospective study. Samples were examined for uropathogens by standard bacteriological procedures. VITEK-2 automated microbiology system was used to identify the isolated uropathogens and determine the susceptibility of E. coli and Klebsiella spp. isolates to antimicrobials. In addition, phenotypically ESBLs-positive E. coli isolates were tested for ESBLs genes by PCR.ResultsThe present study enrolled 1,790 patients with UTIs. Uropathogens were found in 371 (20.7%) urine specimens examined. Mixed pathogens were detected in two specimens with 373 total pathogens isolated. E. coli and Klebsiella spp. were the predominant uropathogens at 55.8% (208/373) and 18.5% (69/373), respectively. Other pathogens were detected in 25.7% (96/373) of urine samples. Of the E. coli and Klebsiella spp. tested, 69.2 and 100% were resistant to ampicillin, 6.7 and 33.3% to ceftriaxone, and 23.1 and 17.4% to ciprofloxacin, respectively. MDR (resistance to ≥3 antimicrobial groups) was found in 69 (33.2%) of E. coli and in 29 (42%) of Klebsiella spp. isolates. ESBLs were detected phenotypically in 14 (6.7%) of E. coli and in 15 (21.7%) of Klebsiella spp. isolates. Thirteen out of the 14 phenotypically ESBL-positive E. coli were positive for ESBL genes by PCR. bla TEM gene was detected in seven isolates, bla OXA gene in 10 isolates and bla CTX-M gene in six isolates. bla SHV gene was not detected in the present study.ConclusionThe isolation of MDR ESBL-producing uropathogens undoubtedly will limit the choices clinicians have to treat their patients with UTIs. Therefore, there is an urgent need for surveillance studies on antimicrobial resistance and prevalence of ESBLs among uropathogens to guide the clinical treatment of UTIs in Libya in the future.

show abstract

Section: Discussionmentioning

confidence: 99%

Multidrug resistance and extended-spectrum β-lactamases genes amongEscherichia colifrom patients with urinary tract infections in Northwestern Libya

Abujnah

Zorgani

Sabri

et al. 2015

Libyan Journal of Medicine

View full text Add to dashboard Cite

show abstract

“…A seven-year study (2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012) at a tertiary care hospital in suburban New York City, USA concluded rapid spread of CTX-M enzyme from 0% (2005)(2006)(2007)(2008) to 5% (2009) to 34.3% (2011) (Wang et al, 2013). Similarly, 25% prevalence of CTX-M gene in Japan (Harada et al, 2013), 41% in China (Du et al, 2014) has been reported. These figures are much low as compared to our study (94.5%).…”

Section: Resultsmentioning

confidence: 99%

Optimization of PCR for rapid detection of CTX-M gene in ESBL producing Klebsiella pneumoniae clinical isolates from Punjab, Pakistan

Bashir, R.,

Zaib, N.,

Altaf, I.

et al. 2016

MJM

View full text Add to dashboard Cite

Aims:The study was carried out firstly, to evaluate the prevalence of extended spectrum beta lactamase (ESBL), multidrug resistant Klebsiella pneumoniae isolates from Punjab, Pakistan and secondly, to characterize the genotypes of their beta lactamase producing enzymes and optimization of PCR based method for rapid and authentic detection of antibiotic resistant gene. Methodology and results: Two hundred of K. pneumonia strains were isolated from different clinical samples. Blood and MacConkey Agar were used to isolate and identify bacterial microorganisms while Muller Hinton Agar was used to evaluate the antimicrobial susceptibility against different antibiotics as per CLSI 2012 guidelines. ESBL producing bacteria were screened by double disk synergy /combination disk test. PCR was optimized and performed for resistant gene (CTX-M). The results showed that most of the isolates were resistant to multiple antibiotic including cephalosporin, aztreonam, sulphamethoxazole/trimethoprim, ciprofloxacin, doxycyclin and were sensitive to imipenam and amikacin. Frequency of CTX-M gene in ESBL producing K. pneumoniae was 94%. Conclusion, significance and impact of study: Based on the finding of this study it is suggested that prevalence of CTX-M gene (95%) is very high among ESBL producing isolates. Therefore, PCR based method may help clinicians for rapid detection and treatment of patients by choosing right medication against the resistant bacteria as early as possible.

show abstract

“…Yazdi et al, [28], Turkey, found that 68.8% of ESBL producer E. coli isolates were carrying CTX-M genotype. Harada et al, [29], Japan reported that ESBL positive E. coli carrying CTX-M genotype in 25% of their tested isolates, in particular CTX-M-9 group. Kang et al, [30], Korea, study shows that CTX-M was predominant and form 76.7% in ESBL producer E. coli.…”

Section: Figure (4) Molecular Detection Of Bla Shv Antibiotic Resistmentioning

confidence: 94%

“…Thus this study finding demonstrated a 6 trends of gene combination and none of the 15 tested E. coli isolates harbouring a single gene. Harada et al, [29], Japan, found that the predominant genotype was CTX-M + TEM which forms 48.3% for E. coli. Single TEM or SHV or CTX-M accounted for 8.1%, 3.1% and 28.2% respectively, while TEM+SHV genotypes form 1.3% and CMX-M + SHV genotypes form 0.6%.…”

Section: Figure (4) Molecular Detection Of Bla Shv Antibiotic Resistmentioning

confidence: 99%

“…Thus present study data indicated that OXA gene demonstrated in 100% of ESBL E. coli uropathogenic isolates tested in this study, while TEM gene presented in 66.7% of the isolates and 60% were harbouring SHV gene. Previous studies reported variable gene frequencies, 76% for OXA in Ireland [31], while TEM gene reported in 86.24% of E. coli ESBL isolates in Turkey [28], 40% in Japan [29], 34% in Ireland [31], 16.81% in Iran [36], 10% in Iraq [33], 5% in Kenya [37], and 0.9% in Japan [15]. But TEM genotype was found in 80% of E. coli ESBL isolates in Japan [29], 72.23% in Turkey [28], 12.1% in Iran [36], 3.33% south Korea [32], 3% in Ireland [31], 3% in Kenya [37] and 2.8% in Japan [15].…”

Section: Figure (4) Molecular Detection Of Bla Shv Antibiotic Resistmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterisation of ESBL producer E. coli

Alsamarai¹,

Khorshed²,

Weli³

2018

IJMS

View full text Add to dashboard Cite

Background: Antibiotic resistance emerged as clinical problem challenge the effective treatment of infections. Virulence factor may play an important role in the influence of antimicrobial resistance. Objective: To determine the frequency of resistance gene in E. coli clinical isolates from women with urinary tract infection. Materials and Methods: Fifteen E.coli clinical isolates were tested by PCR to determine their molecular characterization. Results: The bla CTX –M gene was not detected in 6.7% out of the tested 15 E. coli clinical isolates from women with urinary tract infection. However, bla OXA gene was detected in all E. coli tested clinical isolates from pregnant women, female student and diabetic women with urinary tract infection. While bla TEM gene and bla SHV gene were not detected in 33.3% and 40% out of the tested E. coli clinical isolates respectively. Conclusions: Four types of ESBL genes were detected, and shows new trend of distribution, which indicated the predominance of OXA and CTX-M genes.

show abstract

P152 Clinical and molecular epidemiology of ESBL producing Klebsiella pneumoniae and Escherichia coli in a tertiary hospital

Cited by 6 publications

References 0 publications

Multidrug resistance and extended-spectrum β-lactamases genes amongEscherichia colifrom patients with urinary tract infections in Northwestern Libya

Multidrug resistance and extended-spectrum β-lactamases genes amongEscherichia colifrom patients with urinary tract infections in Northwestern Libya

Optimization of PCR for rapid detection of CTX-M gene in ESBL producing Klebsiella pneumoniae clinical isolates from Punjab, Pakistan

Molecular Characterisation of ESBL producer E. coli

Contact Info

Product

Resources

About