P121 The anti proliferative effect of Aloin and Aloe emodin on MCF-7 and MDA-MB-231 breast cancer cells

Daud, Madiha; Ibrahim, M; Froemming, Gabriele Ruth Anisah; Hasani, Narimah Ah

doi:10.1016/s0960-9776(11)70065-0

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2013

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“…The species in Aloe vera L. have been used as ethnic medicines in many different countries for centuries, possessing functions, such as anti-cancer, anti-inflammatory, anti-virus, evacuating, protecting liver, and increasing immunity [1]. Aloe vera L. has been most commonly used as medicine, healthy foods, and cosmetics nowadays [2][3][4]. The active components of aloe include anthraquinones, chromones, polysaccharides, enzymes and, so on [5][6].…”

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confidence: 99%

Isolation and Purification of Aloin a and Aloin B by High-Speed Counter-Current Chromatography

Tang

Chen

et al. 2013

AMR

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High-speed counter-current chromatography(HSCCC) was successfully used for isolation and purification of Aloin A and Aloin B from the crude methanol extract of Aloe with a two-phase solvent system composed of chloroform–methanol–n-butylalcohol-water at an optimized volume ratio of 4:3:1:2 (v/v/v/v). The lower phase was used as the mobile phase in the head to tail elution mode. The preparative HSCCC separation was performed on 180 mg of the crude extract yielding pure Aloin A(18mg) and Aloin B(16mg) at purities of 95.2% and 96.8%, respectively, as determined by high performance liquid chromatography (HPLC). HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.

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