P12
Measurement of skin edema by a dielectric technique (MoistureMeter‐D)

Abstract

To understand the mechanisms involved in immunological tolerance to skin-associated proteins, we have developed trangenic (Tg) mice that express a model self antigen, membrane-bound ABSTRACTS 125 FS01.3 Disperse (yes), orange (yes), 3 (no): what do we test in textile dye dermatitis?Para-phenylenediamine (PPD), an arylamine dye, is a strong allergen causing allergic contact dermatitis. Cytokines such as TNF-a and IL-1beta are key mediators in the initiation of this reaction. Both cytokines are predominantly produced by stimulated monocytes and macroghages. We investigated the responses of PPD and Bandrowski's base (BB), an autoxidation product of PPD in human monocytes. We isolated monocytes from healthy volunteers and incubated them with the allergens. TNF-a and IL-1beta mRNA expression and protein levels were estimated after 45 min, 2 h, 4 h and 24 h after allergen contact. IL-1beta and TNF-alpha were measured in cell culture supernatants by ELISA (n ¼ 7) and mRNA expression was determined by real-time RT-PCR. We found that PPD reduced TNF-a protein secretion by 20-69.9% (n ¼ 6). Further, IL-1beta levels were decreased by 44-98%. The same tendency was found studying IL-1beta and TNF-a mRNA steady state levels (n ¼ 3; 1 h incubation). These effects were substance-specific and not found for PPD derivatives nor for the autoxidation product BB. These findings suggest that PPD may specifically modify immune responses by directly infering with the cellular proinflammatory cytokine network.

Assessment of skin barrier function in podoconiosis: measurement of stratum corneum hydration and transepidermal water loss

“…This would help us to determine whether the clear SC hydration changes we have measured are a unique feature of podoconiosis. Comparison with previous SC hydration and TEWL data from studies of other diseases would also be interesting 15–20 . However, because the equipment, populations and environment are different in each study, it is unlikely that they would be meaningful.…”

“…Comparison with previous SC hydration and TEWL data from studies of other diseases would also be interesting. [15][16][17][18][19][20] However, because the equipment, populations and environment are different in each study, it is unlikely that they would be meaningful.…”

“…Rising TEWL and falling SC hydration have also been shown in these conditions to correlate well with increasing disease severity 15–18 . To the best of our knowledge, TEWL has not previously been measured in patients with lymphoedema, and although SC hydration has been studied in such patients, it was used only as a surrogate measure for tissue oedema 19,20 . We set out to investigate SC hydration and TEWL in patients with podoconiosis compared with controls.…”

Subepidermal moisture surrounding pressure ulcers in persons with a spinal cord injury: A pilot study

“…Advantages of using radiofrequency waves to measure edema include water specificity, the potential for the device to be handheld, and the lack of ionizing radiation. Likewise, a variety of terms have been used by the authors of studies using these devices to describe what they were measuring, including "free water content", 8 "tissue dielectric constant", 42 "skin tissue water", 43 "changes of tissue water", 44 "tissue water content", 45 "water content", 46 "tissue water", 47 "tissue edema", 48,49 "skin edema", 50 and "subepidermal moisture". 51,52 In this manuscript, the term "subepidermal…”