P1024 Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains isolated from drinking and recreational, salmaster waters

Cernat, Ramona; Balotescu, Carmen; Ivanescu, D.; Nedelcu, D.; Lazăr, Veronica; Bucur, Marcela; Valeanu, D.; Tudorache, R.; Mitache, Mihaela Magdalena; Dragoescu, M.

doi:10.1016/s0924-8579(07)70865-8

Cited by 12 publications

(8 citation statements)

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“…Previous studies have revealed the occurrence of erythromycin resistant bacteria in drinking water (Faria et al, 2009), and our results further indicated that the prevalence of resistance genes ermA and ermB in the finished water of the plant. Additionally, this study showed that aphA2 conferring aminoglycoside resistance was abundant in intestinal bacteria and environmental DNA of the drinking water, which is supported by a previous study demonstrating that aphA2 was present in Escherichia coli isolated from drinking water (Cernat et al, 2007). Few studies have been conducted to quantitatively assess the effects of chlorination on ARGs in drinking water.…”

Section: Discussionsupporting

confidence: 80%

Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water

et al. 2013

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Section: Discussionsupporting

confidence: 80%

Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water

et al. 2013

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“…The Xinfengjiang Reservoir (S29, less impacted by human activities) used as a drinking water source of the Pearl River Delta region was found to have the lowest total concentrations of ARGs in both seasons, and also observed to have the lowest abundance for most of the individual ARGs determined in the present study. Previous studies also showed detection of ARB and ARGs in drinking water (Cernat et al, 2007;Faria et al, 2009;Koksal et al, 2007;Ram et al, 2008), suggesting that some ARB and ARGs can survive drinking water treatment processes. It is documented that microorganisms can acquire ARGs in the environment with the mechanisms of plasmids, integrons, and transposons (Andersson and Levin, 1999;Kruse and Sorum, 1994;Pruden et al, 2006).…”

Section: Spatial Distribution Of the Args In The River Basinmentioning

confidence: 81%

Contamination profiles of antibiotic resistance genes in the sediments at a catchment scale

Pan

Ying

et al. 2014

Science of The Total Environment

116

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“…11, 51 The aadA group of genes encodes resistance to streptomycin and spectinomycin, whereas the dfr group encodes resistance to trimethoprim. In the present study, 50% of the Enterobacteriaceae isolates from the fish farm samples were resistant to trimethoprim and 43% resistant to sulfamethoxazole/ trimethoprim (SXT).…”

mentioning

confidence: 99%

Occurrence of antibiotic resistance and characterization of resistance genes and integrons in Enterobacteriaceae isolated from integrated fish farms in south China

Ying

Tao

et al. 2011

J. Environ. Monit.

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Antibiotics are still widely applied in animal husbandry to prevent diseases and used as feed additives to promote animal growth. This could result in antibiotic resistance to bacteria and antibiotic residues in animals. In this paper, Enterobacteriaceae isolated from four integrated fish farms in Zhongshan, South China were tested for antibiotic resistance, tetracycline resistance genes, sulfonamide resistance genes, and class 1 integrons. The Kirby-Bauer disk diffusion method and polymerase chain reaction (PCR) assays were carried out to test antibiotic susceptibility and resistance genes, respectively. Relatively high antibiotic resistance frequencies were found, especially for ampicillin (80%), tetracycline (52%), and trimethoprim (50%). Out of 203 Enterobacteriaceae isolates, 98.5% were resistant to one or more antibiotics tested. Multiple antibiotic resistance (MAR) was found highest in animal manures with a MAR index of 0.56. Tetracycline resistance genes (tet(A), tet(C)) and sulfonamide resistance genes (sul2) were detected in more than 50% of the isolates. The intI1 gene was found in 170 isolates (83.7%). Both classic and non-classic class 1 integrons were found. Four genes, aadA5, aadA22, dfr2, and dfrA17, were detected. To our knowledge, this is the first report for molecular characterization of antibiotic resistance genes in Enterobacteriaceae isolated from integrated fish farms in China and the first time that gene cassette array dfrA17-aadA5 has been detected in such fish farms. Results of this study indicated that fish farms may be a reservoir of highly diverse and abundant antibiotic resistant genes and gene cassettes. Integrons may play a key role in multiple antibiotic resistances posing potential health risks to the general public and aquaculture.

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P1024 Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains isolated from drinking and recreational, salmaster waters

Cited by 12 publications

References 0 publications

Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water

Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water

Contamination profiles of antibiotic resistance genes in the sediments at a catchment scale

Occurrence of antibiotic resistance and characterization of resistance genes and integrons in Enterobacteriaceae isolated from integrated fish farms in south China

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