P102 Differential antiviral effect of porcine interferon alpha subtypes on classical swine fever virus infection of porcine monocytes

Sosan, Olubukola; Graham, Simon P.; Everett, Helen; Crudgington, Bentley; Bodman‐Smith, Kikki; Crooke, Helen

doi:10.1016/j.cyto.2012.06.192

Cited by 1 publication

(1 citation statement)

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“…Mag-Pam2Cys induced a modest, but statistically significant, enhanced expression of IFN-α1, IFN-α2, IFN-α5/6, IFN-α9, and IFN-α16. These porcine IFNs exhibited direct antiviral activities towards many viruses, including PRRSV [ 54 ], CSFV [ 57 ], and pseudorabies [ 53 ], and presented immunomodulatory properties (inducing MHC I and MHC II upregulation) [ 53 ]. The induction of several type I IFNs, alongside proinflammatory cytokines, suggests that Mag-Pam2Cys would promote host defense responses able to protect the host from microbial infection.…”

Section: Discussionmentioning

confidence: 99%

Targeting Toll-Like Receptor 2: Polarization of Porcine Macrophages by a Mycoplasma-Derived Pam2cys Lipopeptide

et al. 2021

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Toll-like receptor 2 (TLR2) ligands are attracting increasing attention as prophylactic and immunotherapeutic agents against pathogens and tumors. We previously observed that a synthetic diacylated lipopeptide based on a surface protein of Mycoplasma agalactiae (Mag-Pam2Cys) strongly activated innate immune cells, including porcine monocyte-derived macrophages (moMΦ). In this study, we utilized confocal microscopy, flow cytometry, multiplex cytokine ELISA, and RT-qPCR to conduct a comprehensive analysis of the effects of scalar doses of Mag-Pam2Cys on porcine moMΦ. We observed enhanced expression of activation markers (MHC class I, MHC class II DR, CD25), increased phagocytotic activity, and release of IL-12 and proinflammatory cytokines. Mag-Pam2Cys also upregulated the gene expression of several IFN-α subtypes, p65, NOS2, and molecules with antimicrobial activities (CD14, beta defensin 1). Overall, our data showed that Mag-Pam2Cys polarized porcine macrophages towards a proinflammatory antimicrobial phenotype. However, Mag-Pam2Cys downregulated the expression of IFN-α3, six TLRs (TLR3, -4, -5, -7, -8, -9), and did not interfere with macrophage polarization induced by the immunosuppressive IL-10, suggesting that the inflammatory activity evoked by Mag-Pam2Cys could be regulated to avoid potentially harmful consequences. We hope that our in vitro results will lay the foundation for the further evaluation of this diacylated lipopeptide as an immunopotentiator in vivo.

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