P1 (Adenosine) Purinoceptor Assays

Mustafa, S. Jamal; Ansari, Hena A; Abebe, Worku

doi:10.1002/0471141755.ph0407s45

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…1 nM: 3 nM: 10 nM: 30 nM: 100 nM: 300 nM: 1000 nM: 3000 nM 18 , allowing sufficient time of 3 minutes to elapse between each concentration. The relaxation due to ipratropium and test drugs was measured on the tracheal muscles, pre-contracted with carbachol (30 µMol).…”

Section: Measurement Of Tension Due To Standard Drugs and Compoundsmentioning

confidence: 99%

Assessment of Spasmolytic Activity of Alcoholic Extract of Acillea Millefolium, Rubia Cordifolia and Saussurea Lappa in Wistar Albino Rats

Nagar¹,

Kumar²,

Gullaiya³

et al. 2013

Int. Res. J. Pharm.

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The present study was carried out to evaluate the potential of alcoholic extract of anti-inflammatory plants viz. Achillea millefolium, Rubia cordifolia and Saussurea lappa for spasmolytic activity in rat model using radnoti organ bath. The relaxant effect of all extracts on pre-contracted rat tracheal chain by carbachol (30 µM) had been screened. Isometric contractions of isolated rat tracheas were recorded at 1.4 g resting tension and carbachol dose-response curves were performed. EC50 values (27.12, 13.13 and 7.32 µM, respectively) were identified by plotting cumulative concentration response curve and pD2 values (4.48 ± 0.99, 6.06 ± 1.03 and 7.41 ± 0.97, respectively) were calculated for individual alcoholic extract of plant. All extracts were able to relax carbachol precontracted tracheas significantly in a concentration dependent manner. Our results suggested potential role of Achillea millefolium, Rubia cordifolia and Saussurea lappa in asthma for further potential therapeutic and clinical uses.

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Section: Measurement Of Tension Due To Standard Drugs and Compoundsmentioning

confidence: 99%

Assessment of Spasmolytic Activity of Alcoholic Extract of Acillea Millefolium, Rubia Cordifolia and Saussurea Lappa in Wistar Albino Rats

Nagar¹,

Kumar²,

Gullaiya³

et al. 2013

Int. Res. J. Pharm.

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Increased Gi protein signaling potentiates the negative chronotropic effect of adenosine in the SHR right atrium

Rodrigues

Camara

Jurkiewicz

et al. 2018

Naunyn-Schmiedeberg's Arch Pharmacol

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Hypertension is a risk factor for cardiovascular diseases, which have been associated with dysfunction of sympathetic and purinergic neurotransmission. Therefore, herein, we evaluated whether modifications of adenosine receptor signaling may contribute to the cardiac dysfunction observed in hypertension. Isolated right atria from spontaneously hypertensive (SHR) or normotensive Wistar rats (NWR) were used to investigate the influence of adenosine receptor signaling cascade in the cardiac chronotropism. Our results showed that adenosine, the endogenous agonist of adenosine receptors, and CPA, a selective agonist of A receptor, decreased the atrial chronotropism of NWR and SHR in a concentration- and time-dependent manner, culminating in cardiac arrest (0 bpm). Interestingly, a 3-fold lower concentration of adenosine was required to induce the negative chronotropic effect in SHR atria. Pre-incubation of tissues from both strains with DPCPX, a selective A receptor antagonist, inhibited the negative chronotropic effect of CPA, while simultaneous inhibition of A and A receptors, with ZM241385 and MRS1523, did not change the adenosine chronotropic effects. Moreover, 1 μg/ml pertussis toxin, which inactivates the Gαi protein subunit, reduced by 80% the negative chronotropic effects of adenosine in the NWR atrium, with minor effects in SHR tissue. These data indicate that the negative chronotropic effect of adenosine in right atrium depends exclusively on the activation of A receptors. Moreover, the distinct responsiveness of NWR and SHR atria to pertussis toxin reveals that the enhanced negative chronotropic response of SHR right atrium is probably due to an increased activity of Gαi protein-mediated.

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Spasmolytic effect of novel antagonists of the adenosine A2 receptor in isolated guinea pig tracheal smooth muscle

Nagar

Kumar

Gullaiya

et al. 2013

Biomedicine & Aging Pathology

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P1 (Adenosine) Purinoceptor Assays

Cited by 4 publications

References 17 publications

Assessment of Spasmolytic Activity of Alcoholic Extract of Acillea Millefolium, Rubia Cordifolia and Saussurea Lappa in Wistar Albino Rats

Assessment of Spasmolytic Activity of Alcoholic Extract of Acillea Millefolium, Rubia Cordifolia and Saussurea Lappa in Wistar Albino Rats

Increased Gi protein signaling potentiates the negative chronotropic effect of adenosine in the SHR right atrium

Spasmolytic effect of novel antagonists of the adenosine A2 receptor in isolated guinea pig tracheal smooth muscle

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