P073. Hydrogen sulfide regulates the availability of nitric oxide through the formation of a novel nitrosothiol: Implications for cardiovascular function and human disease

Whiteman, Matt; Ali, Yusuf; Li, Ling; Cheong, Yoke Ping; Mok, Ying-Yuan Pamela; Kostetski, Iouri; Chu, Siew Hwa; Siau, Jia Ling; Bhatia, Madhav; Moore, Philip K.

doi:10.1016/j.niox.2006.04.136

Cited by 2 publications

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“…H 2 S formation was detected with the use of a 2-mm H 2 S-selective microelectrode (ISO-H 2 S-2; WPI) attached to an Apollo 1100 Free Radical Analyser (WPI) and shown as picoamps current generated, as described previously. 11 For in vivo experiments, male Sprague-Dawley (weight, 230 to 260 g) rats were anesthetized (2 mL/kg IP) with a mixture of ketamine (24% vol/vol) and medetomidine (16% vol/vol). A femoral artery and vein were cannulated for blood withdrawal and injection of GYY4137 (133 mol/kg IV or IP) or NaHS (20 mol/kg IV), respectively.…”

Section: Chemical Synthesis Of Gyy4137 and Release Of H 2 S In Vitro mentioning

confidence: 99%

Characterization of a Novel, Water-Soluble Hydrogen Sulfide–Releasing Molecule (GYY4137)

Whiteman²,

Guan³

et al. 2008

Circulation

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Background-The potential biological significance of hydrogen sulfide (H 2 S) has attracted growing interest in recent years. The aim of this study was to characterize a novel, water-soluble, slow-releasing H 2 S compound [morpholin-4-ium 4 methoxyphenyl(morpholino) phosphinodithioate (GYY4137)] and evaluate its use as a tool to investigate the cardiovascular biology of this gas. Methods and Results-The acute vasorelaxant effect of drugs was assessed in rat aortic rings and perfused rat kidney in vitro and in the anesthetized rat in vivo. The chronic effect of GYY4137 on blood pressure in normotensive and spontaneously hypertensive rats was determined by tail-cuff plethysmography. GYY4137 released H 2 S slowly both in aqueous solution in vitro and after intravenous or intraperitoneal administration in anesthetized rats in vivo. GYY4137 caused a slow relaxation of rat aortic rings and dilated the perfused rat renal vasculature by opening vascular smooth muscle K ATP channels. GYY4137 did not affect rat heart rate or force of contraction in vitro. GYY4137 exhibited antihypertensive activity as evidenced by ability to reduce N G -nitro-L-arginine methyl ester-evoked hypertension in the anesthetized rat and after chronic (14-day) administration in spontaneously hypertensive rats. Conclusions-These results identify GYY4137 as a slow-releasing H 2 S compound with vasodilator and antihypertensive activity. GYY4137 is likely to prove useful in the study of the many and varied biological effects of H 2 S. GYY4137 may also prove of therapeutic value in cardiovascular disease.

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Section: Chemical Synthesis Of Gyy4137 and Release Of H 2 S In Vitro mentioning

confidence: 99%

Characterization of a Novel, Water-Soluble Hydrogen Sulfide–Releasing Molecule (GYY4137)

Whiteman²,

Guan³

et al. 2008

Circulation

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742

712

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Electron transfer: A primary step in the reactions of sodium hydrosulphide, an H₂S/HS⁻donor

Staško

Brezová

Zalibera

et al. 2009

Free Radical Research

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Endogenously produced H2S/HS(-), a newly found gasotransmitter, is well represented by NaHS. In deoxygenated media it terminated semi-stable oxidant radicals up to stoichiometric ratios of 1:1. In the presence of oxygen the antioxidant activities of NaHS were impaired considerably due to its competitive reactions with molecular oxygen. The primary reaction steps of NaHS were investigated using two different spin traps, 5,5-dimethylpyrroline-N-oxide and sodium 3,5-dibromo-4-nitrosobenzenesulphonate (DBNBS), in protolytic and aprotic solvents (water and dimethylsulphoxide, DMSO) under argon and oxygen. Sulphhydryl radicals (HS(*)/S(* -)) were primarily formed (S(* -) in water and HS(*) in DMSO), probably coupled to the formation of superoxide radical anions. The DBNBS spin trap acted also as an electron acceptor and formed its radical anions in the presence of NaHS. Hence, one of the primary steps in the reactions of sulphides is the electron transfer from H2S/HS(-) species to a suitable acceptor, which may play a fundamental role in their biological functions.

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P073. Hydrogen sulfide regulates the availability of nitric oxide through the formation of a novel nitrosothiol: Implications for cardiovascular function and human disease

Cited by 2 publications

References 0 publications

Characterization of a Novel, Water-Soluble Hydrogen Sulfide–Releasing Molecule (GYY4137)

Characterization of a Novel, Water-Soluble Hydrogen Sulfide–Releasing Molecule (GYY4137)

Electron transfer: A primary step in the reactions of sodium hydrosulphide, an H₂S/HS⁻donor

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P073. Hydrogen sulfide regulates the availability of nitric oxide through the formation of a novel nitrosothiol: Implications for cardiovascular function and human disease

Cited by 2 publications

References 0 publications

Characterization of a Novel, Water-Soluble Hydrogen Sulfide–Releasing Molecule (GYY4137)

Characterization of a Novel, Water-Soluble Hydrogen Sulfide–Releasing Molecule (GYY4137)

Electron transfer: A primary step in the reactions of sodium hydrosulphide, an H2S/HS−donor

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Electron transfer: A primary step in the reactions of sodium hydrosulphide, an H₂S/HS⁻donor