P070 IL-17 and nitric oxide pathways are involved in inflammatory response during Alzheimer’s disease: A study in Algerian patients

Belkhelfa, Mourad; Rafa, Hayet; Medjeber, Oussama; Behairi, Nassima; Abada-Bendib, Myriam; Makrelouf, Mohamed; Belarbi, Soreya; Masmoudi, A.N.; Tazir, M.; Touil-Boukoffa, Chafia

doi:10.1016/j.cyto.2012.06.156

Cited by 1 publication

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“…Similar results have been reported in a Chinese cohort of AD patients [66]. Furthermore, higher systemic levels of IL-17A have been noted in severe AD patients [65], suggesting the probable association of IL-17 with the chronicity of the disease.…”

Section: Discussionsupporting

confidence: 75%

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All-trans-Retinoic Acid Modulates Nitric Oxide and Interleukin-17A Production by Peripheral Blood Mononuclear Cells from Patients with Alzheimer's Disease

Behairi

Belkhelfa

Mesbah-Amroun

et al. 2015

Neuroimmunomodulation

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Background: Alzheimer's disease (AD), the most common form of dementia in the elderly, is a neurodegenerative disorder associated with a complex pathophysiology. It is accepted that inflammation contributes to the pathogenesis of AD. All-trans-retinoic acid (ATRA) is a bioactive derivative of vitamin A that has shown immunomodulatory effects in many immune disorders. Objectives: In our study, we aimed to investigate in vitro immunomodulatory effects of ATRA on inducible nitric oxide synthase (iNOS) expression and interleukin-17A production during AD. Methods: Peripheral blood mononuclear cells (PBMCs) isolated from 30 Algerian AD patients and 14 age-matched nondemented controls were treated (or not) with ATRA. Production of NO and IL-17A in culture media was measured by the modified Griess method and enzyme-linked immunosorbent assay, respectively. Expression of iNOS in PBMCs was examined by fluorescence immunostaining. Results: Our results showed higher spontaneous in vitro production of NO related to overexpression of iNOS in AD patients compared to controls. Remarkably, ATRA treatment showed an important downregulatory effect on NO production and iNOS expression in patients. This effect was associated with a reduction in IL-17A production and increased IL-10 release. Conclusions: Taken together, our results indicate that ATRA exerts anti-inflammatory effects in AD. Furthermore, ATRA represents a promising tool for monitoring inflammatory responses associated with disease progression.

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Section: Discussionsupporting

confidence: 75%

“…Similarly, Yin et al [64] reported that IL-17A and Th17 lymphocytes might be involved in immune pathogenesis caused by the Aβ peptide in AD. Accordingly, we previously showed increased serum levels of IL-17A in Algerian AD patients compared with controls [65]. Similar results have been reported in a Chinese cohort of AD patients [66].…”

Section: Discussionsupporting

confidence: 73%