P07-01. The gut mucosal homing receptor integrin α4β7 forms a complex with CD4 and defines a T cell subset that is highly susceptible to infection by HIV-1

Cicala, Claudia; Martinelli, Elena; Mcnally, JP; Pascucio, M; Patel, Nikita; Jelicic, Katija; Wei, Darmood; Ryk, Donald Van; As, Fauci; Arthos, James

doi:10.1186/1742-4690-6-s3-p99

Cited by 1 publication

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“…5 This specific affinity of gp120 for α4β7 provides a target for clearing virus present in these latent viral sanctuaries. 6 Though antiretroviral agents have contributed to improve disease management, HAART remains inaccessible to HIV viruses, located in the viral reservoirs, resulting in the failure of clearing HIV from these reservoirs and also the development of multidrug resistance. Nanotechnology-based systems for antiretroviral drug delivery offer a real potential of therapeutic improvement, as the nanosystems protect or promote the absorption of anti-HIV drugs, improve their bioavailabilities, and prolong drug residence in desired cellular or anatomic sites, thus reducing needed doses and prolonging time between administrations.…”

Section: Introductionmentioning

confidence: 99%

Zinc-Stabilized Chitosan-Chondroitin Sulfate Nanocomplexes for HIV-1 Infection Inhibition Application

Ensinas

Verrier

et al. 2016

Mol. Pharmaceutics

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Polyelectrolyte complexes (PECs) constituted of chitosan and chondroitin sulfate (ChonS) were formed by the one-shot addition of default amounts of polyanion to an excess of polycation. Key variables of the formulation process (e.g., degree of depolymerization, charge mixing ratio, the concentration, and pH of polyelectrolyte solutions) were optimized based on the PECs sizes and polydispersities. The PECs maintained their colloidal stability at physiological salt concentration and pH thanks to the complexation of polyelectrolytes with zinc(II) ion during the nanoPECs formation process. The PECs were capable of encapsulating an antiretroviral drug tenofovir (TF) with a minimal alteration on the colloidal stability of the dispersion. Moreover, the particle interfaces could efficiently be functionalized with anti-OVA or anti-α4β7 antibodies with conservation of the antibody biorecognition properties over 1 week of storage in PBS at 4 °C. In vitro cytotoxicity studies showed that zinc(II) stabilized chitosan-ChonS nanoPECs were noncytotoxic to human peripheral blood mononuclear cells (PBMCs), and in vitro antiviral activity test demonstrated that nanoparticles formulations led to a dose-dependent reduction of HIV-1 infection. Using nanoparticles as a drug carrier system decreases the IC50 (50% inhibitory concentration) from an aqueous TF of 4.35 μmol·L(-1) to 1.95 μmol·L(-1). Significantly, zinc ions in this system also exhibited a synergistic effect in the antiviral potency. These data suggest that chitosan-ChonS nanoPECs can be promising drug delivery system to improve the antiviral potency of drugs to the viral reservoirs for the treatment of HIV infection.

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Section: Introductionmentioning

confidence: 99%

Zinc-Stabilized Chitosan-Chondroitin Sulfate Nanocomplexes for HIV-1 Infection Inhibition Application

Ensinas

Verrier

et al. 2016

Mol. Pharmaceutics

View full text Add to dashboard Cite

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P07-01. The gut mucosal homing receptor integrin α4β7 forms a complex with CD4 and defines a T cell subset that is highly susceptible to infection by HIV-1

Abstract: Retrovirology Open AccessPoster presentation P07-01. The gut mucosal homing receptor integrin α4β7 forms a complex with CD4 and defines a T cell subset that is highly susceptible to infection by HIV-1

Cited by 1 publication

References 0 publications

Zinc-Stabilized Chitosan-Chondroitin Sulfate Nanocomplexes for HIV-1 Infection Inhibition Application

Zinc-Stabilized Chitosan-Chondroitin Sulfate Nanocomplexes for HIV-1 Infection Inhibition Application

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