P06.10 Lactic acid dampens inflammatory responses elicited by microbial tlr agonists from vaginal and cervical epithelial cells

Abstract

Neither 0.3% L-LA at neutral pH nor acidity alone (HCl, pH 3.9) elicited the abovementioned effects indicating that immune modulation is mediated by the protonated form of L-LA and is not due to low pH. L-LA also reduced PIC-induced secretion of RANTES and MIP3a in all cells, associated with recruitment of HIV target cells to the mucosa. Similar anti-inflammatory effects of L-LA were observed in primary ectocervical cells. Conclusion L-LA found in lactobacillus-dominated vaginal microbiota elicits an anti-inflammatory effect on lower FRT epithelial cells and dampens inflammation induced by microbial TLR agonists suggesting a role in mitigating inflammationinduced HIV susceptibility at the vaginal mucosa.