P059 Using NGS in solid organ transplantation to resolve anti-HLA antibodies to self antigens

Jindra, Peter T.; Tu, Thuydung; Dane, Holly; Clark, Lauren E.; Young, Anna-Marie; Gray, Stacie; Kerman, Ronald H.

doi:10.1016/j.humimm.2017.06.119

Cited by 2 publications

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“…Markers such as STRs, mtDNA and YSTR have already significantly increased their forensic value due to MPS [91]. While MPS of HLA is being routinely utilised in clinical laboratories in fields such as transplantation [92], pharmacology [93] or population studies [61], there have not been any studies testing its suitability for typical forensic samples. However a recent study by Wu et al [66] showed promising results of applying HLA-based typing to kinship analysis.…”

Section: Potential Of Massively Parallel Sequencing In Forensic Analysis Of the Human Leukocyte Antigen Complexmentioning

confidence: 99%

Human Leukocyte Antigen alleles as an aid to STR in complex forensic DNA samples

2020

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Human biological samples with multiple contributors remain one of the most challenging aspects of DNA typing within a forensic science context. With the increasing sensitivity of commercially available kits allowing detection of low template DNA, complex mixtures are now a standard component of forensic DNA evidence. Over the years, various methods and techniques have been developed to try to resolve the issue of mixed profiles. However, forensic DNA analysis has relied on the same markers to generate DNA profiles for the past 30 years causing considerable challenges in the deconvolution of complex mixed samples. The future of resolving complicated DNA mixtures may rely on utilising markers that have been previously applied to gene typing of nonforensic relevance. With Massively Parallel Sequencing (MPS), techniques becoming more popular and accessible even epigenetic markers have become a source of interest for forensic scientists.The aim of this review is to consider the potential of alleles from the Human Leukocyte Antigen (HLA) complex as effective forensic markers. While Massively Parallel Sequencing of HLA is routinely used in clinical laboratories in fields such as transplantation, pharmacology or population studies, there have not been any studies testing its suitability for forensic casework samples.

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Section: Potential Of Massively Parallel Sequencing In Forensic Analysis Of the Human Leukocyte Antigen Complexmentioning

confidence: 99%

Human Leukocyte Antigen alleles as an aid to STR in complex forensic DNA samples

2020

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New challenges, new opportunities: Next generation sequencing and its place in the advancement of HLA typing

2021

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P059 Using NGS in solid organ transplantation to resolve anti-HLA antibodies to self antigens

Cited by 2 publications

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Human Leukocyte Antigen alleles as an aid to STR in complex forensic DNA samples

Human Leukocyte Antigen alleles as an aid to STR in complex forensic DNA samples

New challenges, new opportunities: Next generation sequencing and its place in the advancement of HLA typing

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