P0528 : Transient elastography is safe in patients with pacemakers and implantable cardioverter-defibrillators

Schoelzel, Fabian; Linzbach, Sven; Seeger, Florian; Bojunga, J; Fichtlscherer, Stephan; Rey, Julia; Herrmann, Eva; Zeiher, Andreas M.; Zeuzem, Stefan; Friedrich‐Rust, Mireen

doi:10.1016/s0168-8278(15)30736-4

Cited by 1 publication

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“…2A). It was previously speculated that CAM treatment could impact cccDNA transcription and/or posttranscriptional events (40). To further analyze this potential MoA of JNJ-827 and JNJ-890 compared to other CAMs, we made use of dHepaRG that can be maintained for weeks or months in culture in contrast to PHHs, which quickly dedifferentiate when cultured ex vivo.…”

Section: Resultsmentioning

confidence: 99%

Novel Potent Capsid Assembly Modulators Regulate Multiple Steps of the Hepatitis B Virus Life Cycle

Lahlali

Berke

Vergauwen

et al. 2018

Antimicrob Agents Chemother

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The assembly of hepatitis B virus (HBV) core protein (HBc) into capsids represents a critical step of viral replication. HBc has multiple functions during the HBV life cycle, which makes it an attractive target for antiviral therapies. Capsid assembly modulators (CAMs) induce the formation of empty capsid or aberrant capsid devoid of pregenomic RNA (pgRNA) and finally block relaxed circular DNA neosynthesis and virion progeny. In this study, the novel CAMs JNJ-827 and JNJ-890 were found to be potent inhibitors of HBV replication with respective half-maximal effective concentrations of 4.7 and 66 nM, respectively, in HepG2.117 cells. Antiviral profiling in differentiated HepaRG (dHepaRG) cells and primary human hepatocytes revealed that these compounds efficiently inhibited HBV replication, as well as establishment of covalently closed circular DNA (cccDNA). In addition to these two known effects of CAMs, we observed for the first time that a CAM, here JNJ-827, when added postinfection for a short-term period, significantly reduced hepatitis B e antigen (HBeAg) secretion without affecting the levels of cccDNA amount, transcription, and hepatitis B surface antigen (HBsAg) secretion. This inhibitory activity resulted from a direct effect of JNJ-827 on HBeAg biogenesis. In a long-term treatment condition using persistently infected dHepaRG cells, JNJ-827 and JNJ-890 reduced HBsAg concomitantly with a decrease in viral total RNA and pgRNA levels. Altogether, these data demonstrate that some CAMs could interfere with multiple functions of HBc in the viral life cycle.

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Section: Resultsmentioning

confidence: 99%