P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity

Kozlowska-Makulska, A.; Guilley, H.; Szyndel, Marek S.; Beuve, Monique; Lemaire, Olivier; Herrbach, Étienne; Bouzoubaa, S.

doi:10.1099/vir.0.016360-0

Cited by 50 publications

(45 citation statements)

References 48 publications

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“…This is ORF0 encodes a putative protein of 28.1 kDa (P0). In other poleroviruses, this protein has RNA silencing suppressor activity [22][23][24] and we have also demonstrated this for TVDV P0 (Mo et al, unpublished results). It has been suggested that the P0 s of cucurbit aphid-borne yellow virus (CABYV) and turnip-yellow virus (TuYV) act as F-box proteins that target an essential component of the host post-transcriptional gene-silencing machinery [25].…”

Section: Genome Organization Of Tvdvsupporting

confidence: 51%

Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus

Chen

2010

Virus Genes

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Tobacco bushy top disease is caused by tobacco bushy top virus (TBTV, a member of the genus Umbravirus) which is dependent on tobacco vein-distorting virus (TVDV) to act as a helper virus encapsidating TBTV and enabling its transmission by aphids. Isometric virions from diseased tobacco plants were purified and disease symptoms were reproduced after experimental aphid transmission. The complete genome of TVDV was determined from cloned RT-PCR products derived from viral RNA. It was 5,920 nucleotides (nts) long and had the six major open reading frames (ORFs) typical of a member of the genus Polerovirus. Sequence comparisons showed that it differed significantly from any of the other species in the genus and this was confirmed by phylogenetic analyses of the RdRp and coat protein. SDS-PAGE analysis of purified virions gave two protein bands of about 26 and 59 kDa both of which reacted strongly in Western blots with antiserum produced to prokaryotically expressed TVDV CP showing that the two forms of the TVDV CP were the only protein components of the capsid.

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Section: Genome Organization Of Tvdvsupporting

confidence: 51%

Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus

Chen

2010

Virus Genes

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“…This is also evidenced by P0 of different poleroviruses (Kozlowska-Makulska et al, 2010), as well as the P25 of several potexviruses (Senshu et al, 2009) that show different levels of RSS activity. The function of ADV P6 remains unknown.…”

Section: Discussionmentioning

confidence: 94%

Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing

2016

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Please cite this article as: Bejerman, Nicolás, Mann, Krin S., Dietzgen, Ralf.G., Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing.Virus Research http://dx.doi. org/10.1016/j.virusres.2016.08.008 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights  Alfalfa dwarf virus P protein has a weak local RSS activity  Alfalfa dwarf virus P strongly suppresses systemic RNA silencing  Alfalfa dwarf virus P interacts with AGO1 and AGO4  Alfalfa dwarf virus P inhibits miRNA-guided AGO1 cleavage  Alfalfa dwarf virus P prevents the production of secondary siRNAs ABSTRACT Plants employ RNA silencing as an innate defense mechanism against viruses. As a counter-defense, plant viruses have evolved to express RNA silencing suppressor proteins (RSS), which target one or more steps of the silencing pathway. In this study, we show that the phosphoprotein (P) encoded by the negative-sense RNA virus alfalfa dwarf virus (ADV), a species of the genus Cytorhabdovirus, family Rhabdoviridae, is a suppressor of RNA silencing. ADV P has a relatively weak local RSS activity, and does not prevent siRNA accumulation. On the other hand, ADV P strongly suppresses systemic RNA silencing, but does not interfere with the short-distance spread of silencing, which is consistent with its lack of inhibition of siRNA accumulation. The mechanism of suppression appears to involve ADV P binding to RNA-induced silencing complex proteins AGO1 and AGO4 as shown in protein-protein interaction assays when ectopically expressed.In planta, we demonstrate that ADV P likely functions by inhibiting miRNA-guided AGO1cleavage and prevents transitive amplification by repressing the production of secondary siRNAs. As recently described for lettuce necrotic yellows cytorhabdovirus P, but in contrast to other viral RSS known to disrupt AGO activity, ADV P sequence does not contain any recognizable GW/WG or F-box motifs, which suggests that cytorhabdovirus P proteins may use alternative motifs to bind to AGO proteins.

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“…Such isolate-dependent variability in the SSP activity was already observed for other poleroviral P0 proteins. For instance P0 encoded by some isolates of beet mild yellowing virus (BMYV) were reported to lack silencing suppression activity while other isolates of BMYV were fully competent in RNA silencing inhibition (Klein et al, 2014;Kozlowska-Makulska et al, 2010). Similarly Pfeffer et al (2002) reported a weak SSP activity for P0 encoded by a Dutch isolate of potato leafroll virus (PLRV), while Fusaro et al (2012) and Zhuo et al (2014) showed that P0 of an Australian and an Inner-Mongolian isolate respectively were strong inhibitors of RNA silencing.…”

Section: Discussionmentioning

confidence: 95%

Mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the P0 protein

2015

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P0 proteins of European beet-infecting poleroviruses display variable RNA silencing suppression activity

Cited by 50 publications

References 48 publications

Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus

Complete nucleotide sequence and genome organization of a Chinese isolate of Tobacco vein distorting virus

Alfalfa dwarf cytorhabdovirus P protein is a local and systemic RNA silencing supressor which inhibits programmed RISC activity and prevents transitive amplification of RNA silencing

Mild and severe cereal yellow dwarf viruses differ in silencing suppressor efficiency of the P0 protein

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