P-Selectin Imaging in Cardiovascular Disease: What You See Is What You Get?

Boersma, Hendrikus H.; Haas, Hans J. de; Reutelingsperger, Chris; Slart, Riemer H. J. A.

doi:10.2967/jnumed.111.090506

Cited by 4 publications

(3 citation statements)

References 15 publications

(35 reference statements)

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“…Some molecular probes have been developed to image these biomarkers in preclinical atherosclerosis models with promising results. However, their translational potentials remain to be determined (11,12). Although the clinical tracer 18 F-FDG has been widely used to evaluate the elevated metabolic activity in atherosclerosis, it is essentially a nonspecific tracer and has technical challenges to detect the lesions in coronary arteries because of the size of the vessel and myocardial uptake (4,8,13).…”

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confidence: 99%

PET/CT Imaging of Chemokine Receptor CCR5 in Vascular Injury Model Using Targeted Nanoparticle

et al. 2014

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Inflammation plays important roles at all stages of atherosclerosis. Chemokine systems have major effects on the initiation and progression of atherosclerosis by controlling the trafficking of inflammatory cells in vivo through interaction with their receptors. Chemokine receptor 5 (CCR5) has been reported to be an active participant in the late stage of atherosclerosis and has the potential as a prognostic biomarker for plaque stability. However, its diagnostic potential has not yet been explored. The purpose of this study was to develop a targeted nanoparticle for sensitive and specific PET/CT imaging of the CCR5 receptor in an apolipoprotein E knock-out (ApoE−/−) mouse vascular injury model. Methods The D-Ala1-peptide T-amide (DAPTA) peptide was selected as a targeting ligand for the CCR5 receptor. Through controlled conjugation and polymerization, a biocompatible poly(methyl methacrylate)-core/polyethylene glycol-shell amphiphilic comblike nanoparticle was prepared and labeled with 64Cu for CCR5 imaging in the ApoE−/− wire-injury model. Immunohistochemistry, histology, and real-time reverse transcription polymerase chain reaction (RT-PCR) were performed to assess the disease progression and upregulation of CCR5 receptor. Results The 64Cu-DOTA-DAPTA tracer showed specific PET imaging of CCR5 in the ApoE−/− mice. The targeted 64Cu-DOTA-DAPTA-comb nanoparticles showed extended blood signal and optimized biodistribution. The tracer uptake analysis showed significantly higher accumulations at the injury lesions than those acquired from the sham-operated sites. The competitive PET receptor blocking studies confirmed the CCR5 receptor–specific uptake. The assessment of 64Cu-DOTA-DAPTA-comb in C57BL/6 mice and 64Cu-DOTA-comb in ApoE−/− mice verified low nonspecific nanoparticle uptake. Histology, immunohistochemistry, and RT-PCR analyses verified the upregulation of CCR5 in the progressive atherosclerosis model. Conclusion This work provides a nanoplatform for sensitive and specific detection of CCR5’s physiologic functions in an animal atherosclerosis model.

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confidence: 99%

PET/CT Imaging of Chemokine Receptor CCR5 in Vascular Injury Model Using Targeted Nanoparticle

et al. 2014

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“…The entire cosmos is meant to serve as a sacrament: a material gift from God in and through which we enter into the joy of his heavenly presence". 108 This paper has explored the metaphysical relation between the visible and invisible or material and immaterial, as demonstrated in philosophically infused early Christian theology, to understand how the whole of reality is sacramental-to see how, in Boersma's terms, reality serves as a sacrament; a visible gift of invisible grace. Clearly, this has not simply been a descriptive, historical account of Platonic principles and the early Christian incorporation and transformation of those principles; rather, it has been primarily a conceptual presentationretrieving historically early Christian thought-which serves a theological account of sacramental reality.…”

Section: Conclusion: Reorientation Through a Theology Of Abundancementioning

confidence: 99%

Toward a Natural Theology of Abundance: Reorientation to a Sacramental Reality

Thiessen

2023

Religions

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This paper presents the Christian incorporation and transformation of the philosophical understanding of the relation between the material and immaterial. This transformation centers on the Incarnation of Christ, which changes both the relation or contact between the material and immaterial realms, and human knowledge concerning the immaterial divine presence in material reality. More than a descriptive, historical account, the paper is primarily a conceptual presentation that retrieves the historically early thought of Irenaeus of Lyons and Athanasius of Alexandria to argue for a theological account of sacramental reality. Drawing on the metaphysical function of sacraments—themselves “visible signs of invisible grace”—which convey that there is more to material reality than meets the eye, I argue that this “more” implies what I call “a theology of abundance” that pertains to the whole of created reality, in the abundant nature of creation and of the human being in particular, and especially apparent in human knowing. The implication of such a theology of abundance is a comprehensive reorientation of human being, knowing, and expression in response to the invisible presence of the divine in visible, sacramental reality.

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“…P-selectin, an adhesion molecule, mainly expressed on the surface of activated platelets and injured vascular endothelium, is involved in the pathophysiology of intraluminal thrombus formation and other cardiovascular diseases [4,5]. These features suggest that P-selectin can be an important target for molecular imaging of thrombosis or in acute and chronic cardiovascular diseases [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Functionalized polymer microbubbles as new molecular ultrasound contrast agent to target P-selectin in thrombus

Aid‐Launais

Labour

et al. 2019

Biomaterials

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Thrombotic diseases rarely cause symptoms until advanced stage and sudden death. Thus, early detection of thrombus by a widely spread imaging modality can improve the prognosis and reduce mortality. Here, polymer microbubbles (MBs) made of degradable poly(IsoButylCyanoAcrylate) and functionalized with fucoidan (Fucoidan-MBs) were designed as a new targeted ultrasound contrast agent to image venous thrombus. The physicochemical characterizations demonstrate that the MBs with fucoidan surface exhibit a size of 2-6 μm and stability in suspension at 4°C up to 2 months. MBs exhibit high echogenicity and could be completely burst under high destructive pulse. Flow chamber experiments on activated human platelets show a higher affinity of Fucoidan-MBs than control anionic MBs (CM-Dextran-MBs) under shear stress conditions. In vivo analysis by ultrasound and histological results demonstrate that Fucoidan-MBs are localized in rat venous thrombotic wall, whereas few CM-Dextran-MBs are present. In addition, the binding of Fucoidan-MBs in healthy vein is not observed. Collectively, Fucoidan-MBs appear as a promising functionalized carrier for ultrasound molecular imaging in thrombotic diseases.

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P-Selectin Imaging in Cardiovascular Disease: What You See Is What You Get?

Cited by 4 publications

References 15 publications

PET/CT Imaging of Chemokine Receptor CCR5 in Vascular Injury Model Using Targeted Nanoparticle

PET/CT Imaging of Chemokine Receptor CCR5 in Vascular Injury Model Using Targeted Nanoparticle

Toward a Natural Theology of Abundance: Reorientation to a Sacramental Reality

Functionalized polymer microbubbles as new molecular ultrasound contrast agent to target P-selectin in thrombus

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