P-Protein: A Novel Target for Skin-whitening Agent

Singh, B. K.; Kim, Eun-Ki

doi:10.1007/s12257-018-0241-5

Cited by 3 publications

(2 citation statements)

References 53 publications

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“…Melanin is a colored biopolymer synthesized by melanocytes, whose amount and distribution in the human skin greatly affects skin color [ 1 ]. Although the complete process is not entirely understood [ 2 ], melanin is believed to be synthesized in the melanosome and then transferred to neighboring keratinocytes, which protect their nuclei from harmful ultraviolet radiation [ 3 ]. Therefore, the timely synthesis and subsequent translocation of this pigment molecule is crucial to ensure the safety of the human skin cell DNA from sunlight.…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Melanin Decolorizing Extracellular Peroxidase of Bjerkandera adusta

Baik

Purkayastha

Park

et al. 2021

JoF

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Melanin pigmentation in the human skin results from complicated cellular mechanisms that remain to be entirely understood. Uneven melanin pigmentation has been counteracted by inhibiting synthesis or transfer of melanin in the skin. Recently, an enzymatic approach has been proposed, wherein the melanin in the skin is decolorized using lignin peroxidase. However, not many enzymes are available for decolorizing melanin; the most studied one is lignin peroxidase derived from a lignin degrading fungus, Phanerochaete chrysosporium. Our current study reveals that versatile peroxidase from Bjerkandera adusta can decolorize synthetic melanin. Melanin decolorization was found to be dependent on veratryl alcohol and hydrogen peroxide, but not on Mn2+. The degree of decolorization reached over 40% in 10 min at 37 °C and a pH of 4.5. Optimized storage conditions were slightly different from those for the reaction; crude enzyme preparation was the most stable at 25 °C at pH 5.5. Since the enzyme rapidly lost its activity at 50 °C, stabilizers were screened. As a result, glycerol, a major component in several cosmetic formulations, was found to be a promising excipient. Our results suggest that B. adusta versatile peroxidase can be considered for future cosmetic applications aimed at melanin decolorization.

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Section: Introductionmentioning

confidence: 99%

Functional Characterization of Melanin Decolorizing Extracellular Peroxidase of Bjerkandera adusta

Baik

Purkayastha

Park

et al. 2021

JoF

View full text Add to dashboard Cite

show abstract

“…FEPS inhibited mushroom tyrosinase with the half maximal inhibitory concentration (IC ) of 16.5 mg/ml and dose-dependently inhibited cellular tyrosinase activity (63.9% at 50 μg/ml, and 83.3% at 100 μg/ml) in the cell-free extract of SK-MEL-5 human melanoma cell and α-melanocytestimulating hormone (α-MSH)-stimulated melanin formation in intact SK-MEL-5 human melanoma cell. The IC inhibitors can be used as skin-brightening agents in cosmeceuticals [11,16].…”

Section: Introductionmentioning

confidence: 99%