P-glycoprotein (multi-xenobiotic resistance) and heat shock protein gene expression in the reef coral Montastraea franksi in response to environmental toxicants

Venn, Alexander A.; Quinn, Jennifer A.; Jones, Ross; Bodnár, Andrea

doi:10.1016/j.aquatox.2009.05.003

Cited by 66 publications

(33 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since P-gp is a typical transport protein, this gives rise to the idea that this type of protein might play an important role in the defense of the body against xenobiotics by excreting these compounds out of the cells. Unfortunately, even though the interaction of pesticides with P-gp has already been investigated (Shabbir et al 2005;Venn et al 2009), the protective effect of P-gp on the toxicity of antifouling biocides to the sea urchin embryos in the coastal waters is still poorly characterized. Therefore, in this work, we focus on the toxicities of antifoulants on the sea urchin embryos, particularly to uncover whether P-gp limits the bioavailability of the biocides and contributes to their excretion out of cells.…”

Section: Resultsmentioning

confidence: 99%

“…In recent years, expression of the P-gp transporter has been correlated with cell protection against xenobiotics and environmental pollutants in many different species of insects and marine organisms, and P-gp appears to play a critical role in environmental toxicology (Porretta et al 2008;Venn et al 2009;Bošnjak et al 2010;Sun et al 2009;Xu et al 2010a, b). In the mosquito Aedes caspius, toxicity of two commonly-used insecticides temephos and diflubenzuron has been shown to increase by inhibition of P-gp, suggesting the potential involvement of P-gp in insecticide defense (Porretta et al 2008).…”

Section: Introductionmentioning

confidence: 97%

“…In the mosquito Aedes caspius, toxicity of two commonly-used insecticides temephos and diflubenzuron has been shown to increase by inhibition of P-gp, suggesting the potential involvement of P-gp in insecticide defense (Porretta et al 2008). The interactions between pesticides with P-gp have also been investigated on reef corals, showing that in vitro exposition of copper sulphate and Corexit 9527 could induce the expression of P-gp in the reef corals (Venn et al 2009). Recently, the presence of P-gp has been reported in sea urchin Strongylocentrotus purpuratus, showing that expression of such genes protected the embryos of sea urchin from toxic xenobiotic compounds (Hamdouna et al 2004).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Influence of P-glycoprotein on embryotoxicity of the antifouling biocides to sea urchin (Strongylocentrotus intermedius)

Xue

Wang

et al. 2011

Ecotoxicology

View full text Add to dashboard Cite

P-glycoprotein (P-gp), as an ATP-binding cassette transporter, transports a wide variety of substrates varying from small molecules like steroids to large polypeptides across the cell membrane in human and animals, even in aquatic animals. Although P-gp protein has attracted much attention of research, its effect on the toxicity of environmental toxicants such as antifouling biocides is still poorly understood. The goal of this study is to evaluate whether copper pyrithione (CuPT), Sea-Nine 211, dichlofluanid and tolylfluanid, four widely used antifouling agents, can be transported by P-gp in embryos of sea urchin Strongylocentrotus intermedius in the presence and absence of the P-gp inhibitor verapamil. Cytotoxcicities of Sea-Nine 211 (EC50 = 99 nM, at 4-arm pluteus) and dichlofluanid (EC50 = 144 nM, at multi-cell) are enhanced by the addition of the P-gp inhibitor, indicating that the two biocides are potential P-gp substrates. Tolylfluanid and CuPT are not transported by P-gp out of the cell, since no obvious changes in the cytotoxicities of the two biocides are observed no matter whether verapamil is added or not. In addition, to understand the mechanisms of ligand binding and its interaction with P-gp, a three-dimensional model of the sea urchin P-gp is generated based on the mouse crystal structure by using homology modeling approach. With this model, a flexible docking is performed and the results indicate that Sea-Nine 211 and dichlofluanid share the same binding site with verapamil, composed of key residues Lys677, Lys753, Thr756, Ala780, Met1033 and Phe1037, whereas tolylfluanid and CuPT display totally different binding modes to P-gp. This further demonstrates that Sea-Nine 211 and dichlofluanid are P-gp substrates, which provides us with new insights into the interactions of P-gp with the antifouling contaminants in aquatic invertebrate embryos.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Influence of P-glycoprotein on embryotoxicity of the antifouling biocides to sea urchin (Strongylocentrotus intermedius)

Xue

Wang

et al. 2011

Ecotoxicology

View full text Add to dashboard Cite

show abstract

“…The effects of xenobiotics on the coral animal have been studied in detail, revealing specific genes that respond to heavy metal stress (Morgan et al 2005), oil dispersants, vinblastine (Venn et al 2009), pesticides, and antifouling paints (Downs et al 2012). However, very little is known regarding the influence of xenobiotics on the microbial component of the coral holobiont.…”

Section: Xenobiotic-associated Functional Genesmentioning

confidence: 99%

Microbial functional genes associated with coral health and disease

Pratte¹

2013

Dis. Aquat. Org.

View full text Add to dashboard Cite

Both the incidence and prevalence of coral disease are rapidly increasing, and as a consequence, many studies involving coral microbial associates have been conducted. However, very few of these have considered microbial functional genes. This is an underutilized approach for studying coral disease etiology which is capable of revealing the molecular processes of the coral microbial community. This review presents a summary of the known microbial functional genes that have been linked to coral health and disease. Overall functional gene diversity tended to be lower in healthy corals than diseased or bleached corals, and respiration and photosynthesis functional genes appeared to be crucial to coral health. Genes associated with the nitrogen cycle were the most studied, were highly represented within the coral holobiont, and their expression often shifted in diseased or stressed individuals. Carbon metabolism, such as fatty acid and amino acid catabolism, also tended to shift in unhealthy corals. Genes associated with sulfite respiration as well as dimethylsulfoniopropionate degradation have been detected, although they have yet to be directly associated with coral disease. In addition, genes associated with xenobiotic degradation, antibiotic resistance, virulence, and oxidative stress may all be involved in maintaining coral health. However, the links between these functional genes and their roles in interacting with the coral host are not clear. Continuing identification of coral-associated microbial functional genes within the coral holobiont should facilitate advances in the field of coral health and disease. KEY WORDS: Etiology · Biogeochemical cycling · Holobiont · Functional diversity · Antibiotic resistance Resale or republication not permitted without written consent of the publisherDis Aquat Org 107: [161][162][163][164][165][166][167][168][169][170][171] 2013 diversity and the specific microbial habitat in which the bacteria were detected, as there was with functional gene diversity (Dinsdale et al. 2008). In most cases, the taxonomic identification of a microbe does not elucidate its functional role, giving little indication as to a microbe's importance. To complicate matters further, bacterial species with the same 16S rRNA gene sequence may play different roles in an ecosystem . On the other hand, bacterial species with different 16S rRNA gene sequences may be functionally redundant, with more than one taxonomic group satisfying a single niche (Allison & Martiny 2008). For these reasons, it is crucial that studies begin to examine the role that microbes play in coral health and disease, rather than focus on identification of the microbes present. As an example, 'Type A' Gammaproteobacteria have commonly been associated with healthy fragments of the coral Acropora, and it has been proposed that a disruption in this population may alter the entire microbial community (Kvennefors et al. 2010(Kvennefors et al. , 2012 and increase the coral's vulnerability to disease and bleaching. However, ...

show abstract

“…Measurements were made of the expression of six genes that act in conserved pathways for maintaining protein integrity during stress or to eliminate toxicants, and that are often induced as part of a general stress response (Venn et al, 2009). The product of gene Hsp70-2 belongs to a family of chaperone proteins first identified by their role in responding to heat shock but that also protect against other cellular insults (Feder & Hofmann, 1999;Swindell et al, 2007).…”

Section: Effect Of Piere1 On Stress Biomarkersmentioning

confidence: 99%

An RNA symbiont enhances heat tolerance and secondary homothallism in the oomycete Phytophthora infestans

2010

View full text Add to dashboard Cite

Some strains of Phytophthora infestans, the potato late blight pathogen, harbour a small extrachromosomal RNA called PiERE1. A previous study reported that this RNA symbiont does not noticeably affect its host. Here it is revealed that PiERE1 exerts subtle effects on P. infestans, which result in greater thermotolerance during growth and an increase in secondary homothallism, i.e. oospore formation in the absence of the opposite mating type. The interaction can be considered mutualistic since these traits may increase the fitness of P. infestans in nature. Assays of biomarkers for cellular stress revealed that an Hsp70 chaperone was upregulated by PiERE1. A genome-wide search for more members of the Hsp70 family identified ten belonging to the DnaK subfamily, one in the Hsp110/SSE subfamily, and pseudogenes. Four DnaK subfamily genes encoding predicted cytoplasmic or endoplasmic reticulum proteins were upregulated in strains harbouring PiERE1. This may explain the greater thermotolerance conferred by the RNA element, and suggests that Hsp70 may be a useful biomarker for testing organisms for the cellular effects of symbiotic elements.

show abstract

P-glycoprotein (multi-xenobiotic resistance) and heat shock protein gene expression in the reef coral Montastraea franksi in response to environmental toxicants

Cited by 66 publications

References 58 publications

Influence of P-glycoprotein on embryotoxicity of the antifouling biocides to sea urchin (Strongylocentrotus intermedius)

Influence of P-glycoprotein on embryotoxicity of the antifouling biocides to sea urchin (Strongylocentrotus intermedius)

Microbial functional genes associated with coral health and disease

An RNA symbiont enhances heat tolerance and secondary homothallism in the oomycete Phytophthora infestans

Contact Info

Product

Resources

About