p-Coumaric acid attenuates alcohol exposed hepatic injury through MAPKs, apoptosis and Nrf2 signaling in experimental models

Ramakrishnan, Sabitha; Nishi, Kumari; Gunasekaran, Vinoth Prasanna; Balupillai, Agilan; Ernest, David; Annamalai, Govindhan; Rajamanickam, Vinothkumar; Perumal, Malliga; Latha, S.; Mathan, Ganeshan

doi:10.1016/j.cbi.2020.109044

Cited by 42 publications

(38 citation statements)

References 57 publications

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“…Propolis extracts inhibit the generation of reactive oxygen species (ROS) through nuclear factor E2-related factor 2 (Nrf2) and activation of antioxidant response element (ARE), responsible for transcription of antioxidant enzymes [ 17 , 18 ]. This mechanism of antioxidant action has also been confirmed for all the chosen representative markers [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Undoubtedly, it results from certain polyphenols synergy, and this synergy will depend on the method of propolis extraction [ 16 ].…”

Section: Discussionsupporting

confidence: 61%

Nonaqueous Polyethylene Glycol as a Safer Alternative to Ethanolic Propolis Extracts with Comparable Antioxidant and Antimicrobial Activity

Šuran

Cepanec²,

Mašek

et al. 2021

Antioxidants

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We compared the chemical composition, antioxidant and antimicrobial activity of two propolis extracts: one obtained with nonaqueous polyethylene glycol, PEG 400 (PgEP), and the other obtained with ethanol (EEP). We analyzed the total phenolic content (TPC) and the concentrations of ten markers of propolis antioxidant activity with HPLC-UV: caffeic acid, p-coumaric acid, trans-ferulic acid, trans-cinnamic acid, kaempferol, apigenin, pinocembrin, chrysin, CAPE, and galangin. Antioxidant activity was tested using DPPH and FRAP assay, and antimicrobial activity was assessed through minimum inhibitory concentrations (MICs) and minimum biofilm eradication concentration (MBEC) determination. Maceration gave the yield of propolis of 25.2 ± 0.08% in EEP, and 21.5 ± 0.24% in PgEP. All ten markers of antioxidant activity were found in both extracts, with all marker concentrations, except kaempferol, higher in EEP. There was no significant difference between the TPC and antioxidant activity of the PgEP and the EEP extract; TPC of PgEP was 16.78 ± 0.23 mg/mL, while EEP had TPC of 15.92 ± 0.78 mg/mL. Both extracts had antimicrobial activity against most investigated pathogens and Staphylococcus aureus, Acinetobacter baumannii, and Escherichia coli biofilms. EEP was more effective against all tested susceptible pathogens, except E. coli, possibly due to higher content of kaempferol in PgEP relative to other polyphenols. Nonaqueous PEG 400 could be used for propolis extraction. It gives extracts with comparable concentrations of antioxidants and has a good antioxidant and antimicrobial activity. It is a safe excipient, convenient for pediatric and veterinary formulations.

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Section: Discussionsupporting

confidence: 61%

Nonaqueous Polyethylene Glycol as a Safer Alternative to Ethanolic Propolis Extracts with Comparable Antioxidant and Antimicrobial Activity

Šuran

Cepanec²,

Mašek

et al. 2021

Antioxidants

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“…The results showed that specific kinase inhibitors blocked NET-D1602-and p-coumaric acid-mediated CREB phosphorylation, indicating that activation of phosphorylated CREB by NET-D1602 and p-coumaric acid occurs through PKA, c-Raf/ERKs, and PI3K/mTOR. Furthermore, p-coumaric acid is widely found in plants and exhibits protective effects via the MAP kinase family and Nrf2 signaling [7,32]. Moreover, it was recently reported that p-coumaric acid prevents obesity in brown adipose tissue mediated by the mTORC1/RPS6 pathways [33].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons

Kim

Choi

et al. 2021

Processes

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Corticosterone (CORT)-induced oxidative stress and neurotoxicity can cause neuronal dysfunction and mental disorders. In the present study, we investigated the effects and mechanism of the HP-20 resin fraction of the water extract of Vaccinium bracteatum leaves (NET-D1602) and its bioactive compound p-coumaric acid on neuronal cell damage in SH-SY5Y cells and primary culture of rat cortical cells. NET-D1602 and p-coumaric acid significantly improved cell viability in CORT-induced neurotoxicity in SH-SY5Y cells and primary cultures of rat cortical cells, and increased the activities of antioxidant enzymes (superoxide dismutase and catalase) against CORT-induced neurotoxicity in SH-SY5Y cells. NET-D1602 and p-coumaric acid increased the phosphorylation levels of ERK1/2 and cAMP response element-binding protein (CREB) in cortical neurons. In addition, CREB phosphorylation by NET-D1602 and p-coumaric acid was dramatically reversed by PKA, c-Raf/ERK, PI3K, and mTOR inhibitors. Lastly, we demonstrated the neuroprotective effects of NET-D1602 (3 and 10 μg/mL) and p-coumaric acid (3 and 10 μM) via increased CREB phosphorylation in CORT-induced neurotoxicity mediated via the ERK1/2, Akt, and mTOR pathways. These results suggest that p-coumaric acid is a potential neuroprotective component of NET-D1602, with the ability to protect against CORT-induced neurotoxicity by regulating ERK1/2, Akt, and mTOR-mediated CREB phosphorylation.

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“…[28] Many pieces of evidence have documented that the initiation of apoptosis occurred by the means of reduction of endogenous anti-oxidants depletion or enhanced production of ROS. [29] The cells remain alive with lesser ROS levels, but the relative ROS development drives cell cycle arrest or apoptosis. Presently, ROS altering drugs are a radical phenomenon for inventing new treatment strategies to preclude malignant cells.…”

Section: Discussionmentioning

confidence: 99%

Punicalagin induces ROS‐mediated apoptotic cell death through inhibiting STAT3 translocation in lung cancer A549 cells

Fang¹,

Wang²,

Zhang

et al. 2021

J Biochem & Molecular Tox

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Lung cancer is a noxious disease with substandard overall survival. Despite this, there are several treatment strategies for lung cancer include chemotherapy, radiotherapy, surgery; however, the overall survival remains poor. Punicalagin has been documented as a potential phytomedicine to selectively inhibit the progression and expansion of numerous cancers. In the present study, we evaluated the antiproliferative ability of punicalagin against lung cancer A549 cells by inducing apoptosis by inhibiting STAT-3 activation. Punicalagin induces toxic effects of A549 cells in a dose-associated manner after 24 h treatment.And we also observed that punicalagin (10, 20, and 30 μM) induced reactive oxygen species generation, alters the mitochondrion membrane potential and apoptotic morphological changes in A549 cells. The STAT-3 overexpression regulates apoptosis, proliferation, and angiogenesis. Here, the punicalagin inhibited STAT-3 translocation and thereby induces apoptosis by inhibiting expression Bcl-2 and enhanced expression of Bax, cytochrome-c, caspase-9, and caspase-3 in A549 cells. Hence, we stated that the punicalagin is a possible therapy for non-small cell lung, malignancies. Altogether, the punicalagin is a promising phytomedicine in malignancy treatment and further endeavors are needed to unveil the complete potential.

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p-Coumaric acid attenuates alcohol exposed hepatic injury through MAPKs, apoptosis and Nrf2 signaling in experimental models

Cited by 42 publications

References 57 publications

Nonaqueous Polyethylene Glycol as a Safer Alternative to Ethanolic Propolis Extracts with Comparable Antioxidant and Antimicrobial Activity

Nonaqueous Polyethylene Glycol as a Safer Alternative to Ethanolic Propolis Extracts with Comparable Antioxidant and Antimicrobial Activity

Protective Effects of p-Coumaric Acid Isolated from Vaccinium bracteatum Thunb. Leaf Extract on Corticosterone-Induced Neurotoxicity in SH-SY5Y Cells and Primary Rat Cortical Neurons

Punicalagin induces ROS‐mediated apoptotic cell death through inhibiting STAT3 translocation in lung cancer A549 cells

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