p-Chlorophenoxyisobutyric acid impairs auxin response for gravity-regulated peg formation in cucumber (Cucumis sativus) seedlings

Shimizu, Midori; Miyazawa, Yutaka; Fujii, Nobuharu; Takahashi, Hideyuki

doi:10.1007/s10265-007-0121-0

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…Gravistimulation has been suggested to affect auxin accumulation and subsequent peg formation on the lower side of the transition zone (Takahashi et al, 2000). Application of PCIB to cucumber seedlings inhibited peg formation, decreased auxin-induced CsIAA1 mRNAs, and increased auxin-repressible CsGRP1 mRNA on the lower side of the transition zone (Shimizu et al, 2008). These results confirm the significant role of endogenous auxin in peg formation at the gravistimulated transition zone of cucumber seedlings.…”

Section: Role Of Auxin Signaling Components In Gravity Responsesupporting

confidence: 68%

New Insights to Auxin Signaling through The Use of Small Molecules

Biswas

Oono

2011

Biol. Sci. Space

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Elucidating the perception mechanism of auxin has been a long-standing goal in plant biology. To und e rst and the m e chanism , studies on the chemical structure and activity relationships of auxin-related small molecules have been done for more than half a century. Today, aided mainly by Arabidopsis genetics, researchers have identified the core complexes for auxin perception, including the auxin receptors TIR1/AFBs, SKP2 and ABP1. Forward chemical screening using auxin response markers has also played an important role in identifying small molecules affecting the auxinsignaling pathways and led to the screening of additional auxin related mutants. This approach has helped in identifying several unique molecular components of the auxinsignaling pathways, and discovering that would have been impossible using traditional screening of mutants against auxin. This review is focused on recently acquired knowledge of multiple auxin signaling pathways and related small compounds that can be used to dissect auxin signal transduction pathways. In addition, the involvement of distinct auxinsignaling machineries during gravity response is discussed.

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Section: Role Of Auxin Signaling Components In Gravity Responsesupporting

confidence: 68%

New Insights to Auxin Signaling through The Use of Small Molecules

Biswas

Oono

2011

Biol. Sci. Space

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“…This model is probable, because asymmetric expression of an auxin-inducible gene, CsIAA1 , is detected in the epidermis and cortex across the transition zone. 5,23,24 Regulation of auxin levels in the cortex and epidermis in this way may be an important factor responsible for the lateral placement of peg formation in cucumber seedlings.…”

Section: Discussionmentioning

confidence: 99%

The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy

et al. 2016

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Reorientation of cucumber seedlings induces re-localization of CsPIN1 auxin efflux carriers in endodermal cells of the transition zone between hypocotyl and roots. This study examined whether the re-localization of CsPIN1 was due to the graviresponse. Immunohistochemical analysis indicated that, when cucumber seedlings were grown entirely under microgravity conditions in space, CsPIN1 in endodermal cells was mainly localized to the cell side parallel to the minor axis of the elliptic cross-section of the transition zone. However, when cucumber seeds were germinated in microgravity for 24 h and then exposed to 1g centrifugation in a direction crosswise to the seedling axis for 2 h in space, CsPIN1 was re-localized to the bottom of endodermal cells of the transition zone. These results reveal that the localization of CsPIN1 in endodermal cells changes in response to gravity. Furthermore, our results suggest that the endodermal cell layer becomes a canal by which auxin is laterally transported from the upper to the lower flank in response to gravity. The graviresponse-regulated re-localization of CsPIN1 could be responsible for the decrease in auxin level, and thus for the suppression of peg formation, on the upper side of the transition zone in horizontally placed seedlings of cucumber.

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“…Application of exogenous auxin induces the development of a peg on the upper side of the transition zone in a horizontal position . Furthermore, application of an inhibitor of auxin action, p-chlorophenoxyisobutyric acid, inhibits peg formation (Shimizu et al, 2008). These results indicate that gravity-induced differential auxin-inducible gene expression and suppression of peg development result from a decrease in auxin on the upper side of the transition zone.…”

mentioning

confidence: 81%

“…These results indicate that gravity-induced differential auxin-inducible gene expression and suppression of peg development result from a decrease in auxin on the upper side of the transition zone. By contrast, the lower side of the transition zone maintains the auxin levels required for peg formation (Kamada et al, , 2003Shimizu et al, 2008). Seedlings treated with an auxin transport inhibitor, 2,3,5-triiodobenzoic acid (TIBA), or 9-hydroxyfluorene-9-carbomylic acid block the suppression of peg formation on the upper side and develop a peg on each side of the transition zone, regardless of the position of seedling germination (Kamada et al, 2003).…”

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confidence: 99%

Gravistimulation Changes the Accumulation Pattern of the CsPIN1 Auxin Efflux Facilitator in the Endodermis of the Transition Zone in Cucumber Seedlings

Watanabe

Fujii²,

Yanai³

et al. 2011

Plant Physiology

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Cucumber (Cucumis sativus) seedlings grown in a horizontal position develop a specialized protuberance (or peg) on the lower side of the transition zone between the hypocotyl and the root. This occurs by suppressing peg formation on the upper side via a decrease in auxin resulting from a gravitational response. However, the gravity-stimulated mechanism of inducing asymmetric auxin distribution in the transition zone is poorly understood. The gravity-sensing tissue responsible for regulating auxin distribution in the transition zone is thought to be the endodermal cell. To characterize the gravity-stimulated mechanism, the auxin efflux facilitator PIN-FORMED1 (CsPIN1) in the endodermis was identified and the localization of CsPIN1 proteins during the gravimorphogenesis of cucumber seedlings was examined. Immunohistochemical analysis revealed that the accumulation pattern of CsPIN1 protein in the endodermal cells of the transition zone of cucumber seedlings grown horizontally differed from that of plants grown vertically. Gravistimulation for 30 min prompted changes in the accumulation pattern of CsPIN1 protein in the endodermis as well as the asymmetric distribution of auxin in the transition zone. Furthermore, 2,3,5-triiodobenzoic acid inhibited the differential distribution of auxin as well as changes in the accumulation pattern of CsPIN1 in the endodermis of the transition zone during gravistimulation. These results suggest that the altered pattern of CsPIN1 accumulation in the endodermis in response to gravistimulation influences lateral auxin transport through the endodermis, resulting in asymmetric auxin distribution in the transition zone.

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p-Chlorophenoxyisobutyric acid impairs auxin response for gravity-regulated peg formation in cucumber (Cucumis sativus) seedlings

Cited by 7 publications

References 23 publications

New Insights to Auxin Signaling through The Use of Small Molecules

New Insights to Auxin Signaling through The Use of Small Molecules

The gravity-induced re-localization of auxin efflux carrier CsPIN1 in cucumber seedlings: spaceflight experiments for immunohistochemical microscopy

Gravistimulation Changes the Accumulation Pattern of the CsPIN1 Auxin Efflux Facilitator in the Endodermis of the Transition Zone in Cucumber Seedlings

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