P- and E-selectin use common sites for carbohydrate ligand recognition and cell adhesion.

Erbe, David V.; Watson, Susan R.; Presta, Leonard G.; Wolitzky, Barry A.; Foxall, Carrol; Brandley, Brian K.; Lasky, Laurence A.

doi:10.1083/jcb.120.5.1227

Cited by 154 publications

(101 citation statements)

References 51 publications

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“…The correlation between specificity and residue pair was found in sequence comparisons to be in excellent agreement with other C-type lectin domains of known sequence and monosaccharide specificity (Table 1). Point mutations as well as peptide/mAb inhibition studies (Geng et al, 1991(Geng et al, , 1992 were also used to localize the binding site(s) within the E-and P-selectin molecules (Erbe et al, 1992(Erbe et al, , 1993. However, the possibility of more than 1 binding site present within these adhesion molecules, or additional protein-protein interactions involved in selectinglycoprotein recognition, cannot be ruled out, and should also be considered in the definition of ligands for the collectins.…”

Section: The Iectin Domainmentioning

confidence: 99%

Collectins — soluble proteins containing collagenous regions and lectin domains — and their roles in innate immunity

1994

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The collectins are a group of mammalian lectins containing collagen-like regions. They include mannan binding protein, bovine conglutinin, lung surfactant protein A, lung surfactant protein D, and a newly discovered bovine protein named collectin-43. These proteins share a very similar modular domain composition and overall 3-dimensional structure. They also appear to play similar biological roles in the preimmune defense against microorganisms in both serum and lung surfactant. The close evolutionary relationship between the collectins is further emphasized by a common pattern of exons in their genomic structures and the presence of a gene cluster on chromosome 10 in humans that contains the genes known for the human collectins. Studies on the structure/function relationships within the collectins could provide insight into the properties of a growing number of proteins also containing collagenous regions such as Clq, the hibernation protein, the a-and 0-ficolins, as well as the membrane acetylcholinesterase and the macrophage scavenger receptor.

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Section: The Iectin Domainmentioning

confidence: 99%

Collectins — soluble proteins containing collagenous regions and lectin domains — and their roles in innate immunity

1994

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“…This loose interaction is followed by firm adherence, which is mediated by upregulation of β 2 -integrins (eg, CD11b/CD18) on leukocytes and intercellular adhesion molecule-1 (ICAM-1) on endothelial cells. 2,3 Selectins bind to various ligands on vascular endothelial cells, including CD34, P-selectin glycoprotein ligand-1 (PSGL-1), glycoprotein cell adhesion molecule-1 (GlyCAM-1), and sialyl Lewis x (sLe x ). 4 Each of the 3 selectins binds to sLe x .…”

mentioning

confidence: 99%

Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves

Radi¹,

Caverly²,

Dixon³

et al. 2001

ajvr

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Objective—To determine effects of the selectin inhibitor TBC1269 on neutrophil-mediated pulmonary damage during acute Mannheimia haemolytica-induced pneumonia in newborn calves. Animals—Eighteen 1- to 3-day-old colostrumdeprived calves. Procedure—Mannheimia haemolytica or saline (0.9% NaCl) solution was inoculated in both cranial lung lobes of 12 and 6 calves, respectively. Calves were euthanatized 2 (saline, n = 3; M haemolytica, n = 4) or 6 hours (saline, n = 3; M haemolytica, n = 8) after inoculation. Four M haemolytica-inoculated calves euthanatized at 6 hours also received TBC1269 (25 mg/kg, IV) 30 minutes before and 2 hours after inoculation. Conjugated diene (CD) concentrations, inducible nitric oxide synthase (iNOS) expression, and apoptotic cell counts were determined in lung specimens collected during necropsy. Results—Conjugated diene concentrations were significantly increased in all M haemolytica-inoculated groups, compared with saline-inoculated groups. Calves treated with TBC1269 had decreased concentrations of CD, compared with untreated calves, although the difference was not significant. Number of apoptotic neutrophils and macrophages increased significantly in TBC1269-treated calves, compared with untreated calves. Inducible nitric oxide synthase was expressed by epithelial cells and leukocytes. However, iNOS was less abundant in airway epithelial cells associated with inflammatory exudates. Degree of iNOS expression was similar between TBC1269-treated and untreated calves. Conclusions—Mannheimia haemolytica infection in neonatal calves resulted in pulmonary tissue damage and decreased epithelial cell iNOS expression. The selectin inhibitor TCB1269 altered, but did not completely inhibit, neutrophil-mediated pulmonary damage. ( Am J Vet Res 2001;62:17–22)

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“…The novel fold of this protein family, with its unusually high content of nonclassical secondary structure, illustrates how crucial the identification of a closely related structural template has been for model building of P-selectin. Using the mannose-binding protein as structural template, models of E-selectin (Erbe et al, 1992;Mills, 1993) and P-selectin (Erbe et al, 1993) were also derived by similar approaches.…”

Section: Examples Of Comparative Model Building: P-selectinmentioning

confidence: 99%

Knowledge‐based model building of proteins: Concepts and examples

1993

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We describe how to build protein models from structural templates. Methods to identify structural similarities between proteins in cases of significant, moderate to low, or virtually absent sequence similarity are discussed. The detection and evaluation of structural relationships is emphasized as a central aspect of protein modeling, distinct from the more technical aspects of model building. Computational techniques to generate and complement comparative protein models are also reviewed. Two examples, P-selectin and gp39, are presented to illustrate the derivation of protein model structures and their use in experimental studies.Keywords: protein modeling; protein structure; sequence similarity; sequence-structure compatibility; structural similarityThe gap between the number of available amino acid sequences and three-dimensional structures of proteins elucidated by crystallography or NMR techniques is expanding rapidly. The rate of sequence determination is at least 50-fold higher than the rate of structure determination (Bowie et al., 1991). It is therefore not surprising to note an increasing interest in predictive methods to derive three-dimensional protein models (Thornton et al., 1991;Fetrow & Bryant, 1993;Rost et al., 1993). We will review current protein modeling techniques, with a focus on knowledge-based methods (Blundell et al., 1987;Greer, 1991). Central to the review will be the question of how meaningful template structures for protein modeling can be identified and used for model building. Furthermore, we will report on two recent examples of knowledge-based model building carried out in our laboratory, P-selectin and gp39, the human ligand for CD40, and will discuss the role of these models for the rationalization and design of experiments. Homology versus similarityKnowledge-based model building is often called "modeling by homology." Such modeling techniques start from ~~ ~ ~~

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P- and E-selectin use common sites for carbohydrate ligand recognition and cell adhesion.

Cited by 154 publications

References 51 publications

Collectins — soluble proteins containing collagenous regions and lectin domains — and their roles in innate immunity

Collectins — soluble proteins containing collagenous regions and lectin domains — and their roles in innate immunity

Effects of the synthetic selectin inhibitor TBC1269 on tissue damage during acute Mannheimia haemolytica-induced pneumonia in neonatal calves

Knowledge‐based model building of proteins: Concepts and examples

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