Multi-layer light field displays (MLLFDs) are a promising computational display type that can not only display hologram-like 3D content but will also be well compatible with normal 2D applications. However, the quality of experience measurement for MLLFDs is always an important yet challenging issue. Despite existing research works on MLLFDs, most of them only provide quality of experience results with qualitative evaluation, for example, software simulation of a few 3D images/videos, rather than rigorous quantitative evaluation. This work targets at building a unified software and hardware measurement platform for different MLLFD methods, and comprehensively measuring both objective and subjective performance based on virtual object models. To the best of our knowledge, it is the first time that such performance has been measured for MLLFDs. In addition, to use the existing disclosed virtual object sequences, this paper further proposes three customized virtual models, which are the USAF-E model, the view angle model, and the concave/convex object model for accurate measurement of spatial resolution, viewing angle, and depth resolution. A toolbox for MLLFD measurement with proposed models is also released in this paper. The experimental results demonstrate that our proposed measurement method, models, and toolbox can well measure MLLFDs in different configurations.