P.565 Characterisation of pain-, anxiety- and cognition-related behaviour in the complete Freund's adjuvant model of chronic inflammatory pain in Wistar-Kyoto rats

Ferdousi, Mehnaz; Calcagno, Patricia; Sanchéz, Connie; Smith, Karen; Kelly, J.P.; Roche, M.; Finn, David P.

doi:10.1016/j.euroneuro.2019.09.567

Cited by 2 publications

(3 citation statements)

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“…Since the CFA is applied to promote inflammatory response, we used it to enhance the immune response to the GluN1 peptide (Fontes et al, 2017 ). In other studies, CFA was used to induce chronic pain in rodents, causing movement deficits and mechanical hypersensitivity (Liu et al, 2015 ; Sheahan et al, 2017 ; Ferdousi et al, 2019 ; Pitzer et al, 2019 ). Thus, we could not determine whether the locomotion and anxiety levels in sham and NMDARE group were caused by chronic pain.…”

Section: Discussionmentioning

confidence: 99%

“…We found that LTP in the hippocampal CA1 area of NMDARE mice was significantly impaired compared to that in both naïve and sham mice. According to the literature, subcutaneous injection of CFA can lead to chronic pain accompanied with memory deficits in which the hippocampus plays an important regulatory role (Liu et al, 2015 ; Ferdousi et al, 2019 ; Li et al, 2019 ). Since the sham group was injected with the control drug containing CFA, the degree of LTP in sham mice was less than that of naïve mice, but unsurprisingly the LTP defect of the NMDARE mice was more serious and extensive.…”

Section: Discussionmentioning

confidence: 99%

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A juvenile mouse model of anti-N-methyl-D-aspartate receptor encephalitis by active immunization

He,

Sun,

Zhu

et al. 2023

Front. Mol. Neurosci.

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IntroductionAnti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a common autoimmune encephalitis, and it is associated with psychosis, dyskinesia, and seizures. Anti-NMDAR encephalitis (NMDARE) in juveniles and adults presents different clinical charactreistics. However, the pathogenesis of juvenile anti-NMDAR encephalitis remains unclear, partly because of a lack of suitable animal models.MethodsWe developed a model of juvenile anti-NMDAR encephalitis using active immunization with an amino terminal domain peptide from the GluN1 subunit (GluN1356 − 385) against NMDARs in 3-week-old female C57BL/6J mice.ResultsImmunofluorescence staining suggested that autoantibody levels in the hippocampus increased, and HEK-293T cells staining identified the target of the autoantibodies as GluN1, suggesting that GluN1-specific immunoglobulin G was successfully induced. Behavior assessment showed that the mice suffered significant cognition impairment and sociability reduction, which is similar to what is observed in patients affected by anti-NMDAR encephalitis. The mice also exhibited impaired long-term potentiation in hippocampal CA1. Pilocarpine-induced epilepsy was more severe and had a longer duration, while no spontaneous seizures were observed.ConclusionThe juvenile mouse model for anti-NMDAR encephalitis is of great importance to investigate the pathological mechanism and therapeutic strategies for the disease, and could accelerate the study of autoimmune encephalitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A juvenile mouse model of anti-N-methyl-D-aspartate receptor encephalitis by active immunization

He,

Sun,

Zhu

et al. 2023

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Morris Water Maze 19,20 , Barnes Maze 16 ) and recognition tasks (e.g. novel object recognition [21][22][23] ) have been implemented to measure cognitive function in large cohorts of mice 12,[24][25][26][27][28] , including chemically-induced rodent pain models such as Chemotherapy Induced Peripheral Neuropathy [29][30][31] and Complete Freund's Adjuvant 32 which exhibit a limited period of pain sensitivity. However, these paradigms rely on hippocampalbased spatial memory and the HPA-driven stress-influenced motivated behavior that provide an experimental challenge for evaluating executive function and other subdomains of cognitive behavior.…”

Section: Introductionmentioning

confidence: 99%

Examining Cognitive Performance in Mice using the Open-Source Operant Feeding Device FED3

Murdaugh,

Brown,

Chen

et al. 2024

Preprint

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Cognitive impairments are prevalent in various neurological disorders, including chronic pain conditions, and pose significant therapeutic challenges. Preclinical rodent models serve as valuable tools for investigating the underlying mechanisms of and treatments for cognitive dysfunction. However, factors such as stress, age, sex, and disease duration present challenges to reliably capturing cognitive deficits in rodents. Here, we present a comprehensive and high-throughput protocol utilizing the open-source operant Feeding Experimentation Device 3 (FED3) for assessing cognitive performance in mice. We developed a data pipeline to streamline data compilation and analysis, and established operating conditions for a six-test cognitive battery which can be completed in as few as 20 days. We validated our testing procedures using bilateral orbitofrontal cortical lesions to capture deficits in executive function, and demonstrated the feasibility of assessing cognitive function in aged mice of both sexes to identify genotypic and sex-specific effects. Overall, our findings demonstrate that the FED3 is a versatile tool for evaluating cognitive function in mice, offering a low-cost, high-throughput approach for preclinical studies of neurological disorders. We anticipate that this protocol will facilitate broader implementation of cognitive testing in rodent models and contribute to the understanding and treatment of cognitive dysfunction in neurological diseases.

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P.565 Characterisation of pain-, anxiety- and cognition-related behaviour in the complete Freund's adjuvant model of chronic inflammatory pain in Wistar-Kyoto rats

Cited by 2 publications

References 5 publications

A juvenile mouse model of anti-N-methyl-D-aspartate receptor encephalitis by active immunization

A juvenile mouse model of anti-N-methyl-D-aspartate receptor encephalitis by active immunization

Examining Cognitive Performance in Mice using the Open-Source Operant Feeding Device FED3

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