P.115 The effects of Arginine administration on theamino acid levels and the acute cellular inflammatory response: experimental study after the implantation of prosthetic material in the rat abdominal wall

The repair of incisional hernias has taken advantage of the strength provided by prosthetic mesh grafts, but the best position for inserting the materials has not been conclusively established. Environmental scanning electron microscopy (ESEM) provides imaging of biologic samples with minimal manipulation. We used ESEM for early imaging of the integration response to polypropylene meshes placed in two anatomic positions in the abdominal wall and correlated results with tensiometric studies. Two macroporous polypropylene prostheses were implanted in a rat model--one on the abdominal aponeurotic layer and one on the peritoneal surface--without creating a wall defect. Studies were performed over implantation intervals of 7, 15, and 30 days in strips obtained from the polypropylene fiber-receptor repair tissue interface. Microscopic appearance, tensile strength, percent elongation, and stiffness were evaluated. Meshes implanted on the abdominal aponeurotic layer showed better early tissue incorporation (higher collagen deposition, capillary density, cell accumulation) and increased tensile strength, reflecting tighter anchorage to the abdominal wall. The percent elongation increased from day 7 to day 30 after implantation, mainly in the deep stratum. The ESEM images correlated well with biomechanical results, indicating the potential of this technique as a powerful, effective tool for use in wound-healing studies.

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“…Formalin-fixed tissues were prepared for histologic and immunohistochemieal evaluation using standard techniques [9]. Primary polyclona!…”

Section: Methodsmentioning

confidence: 99%

Early imaging of integration response to polypropylene mesh in abdominal Wall by environmental scanning electron microscopy: Comparison of two placement techniques and correlation with tensiometric studies

et al. 2001

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P.115 The effects of Arginine administration on theamino acid levels and the acute cellular inflammatory response: experimental study after the implantation of prosthetic material in the rat abdominal wall

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Early imaging of integration response to polypropylene mesh in abdominal Wall by environmental scanning electron microscopy: Comparison of two placement techniques and correlation with tensiometric studies

Early imaging of integration response to polypropylene mesh in abdominal Wall by environmental scanning electron microscopy: Comparison of two placement techniques and correlation with tensiometric studies

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