P.1.034 In vivo study in humans on disposition of the enantiomers and metabolites of mirtazapine

Delbressine, L. P. C.; Ml, Dahl; Wildenberg, H. M. Van Den; Kleijn, Huub-Jan

doi:10.1016/s0924-977x(97)88483-0

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“…MTZ is administered in a single daily dose, which range between 15 and 45 mg/day, resulting in plasma concentrations between 5 and 100 ng·mL -1 [2]. The drug is used as a racemic mixture of S (+) and R (-) enantiomers, and the parent compound is responsible for most of the pharmacological activity of MTZ [6]. Its molecular formula is C 17 H 19 N 3 .…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an LC-MS/MS Method for Quantitative Analysis of Mirtazapine in Human Plasma

Chorilli¹,

Bonfílio²,

Louvandini³

et al. 2011

AJAC

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Mirtazapine (MTZ) is an antidepressant drug, which belongs to the chemical class of piperazinoazepines. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of MTZ in plasma at the concentrations associated with therapy. Diazepam (DZP) was used as internal standard, added to 200 μL of plasma sample prior to a liquid-liquid extraction using hexane. Chromatographic separation was achieved on an Agilent ® Eclipse XDB C-18 column (100 × 2.1 mm, 3.5 μm) in isocratic mode at 40˚C. Mobile phase was 10 mM ammonium acetate/acetonitrile/formic acid (60/40/0.1, v/v/v) at a constant flow rate of 0.5 mL·min -1 . The injection volume was 10 L and the total run time was 3.2 min. The method shows selectivity and linearity. The detection and quantitation limits were established at 0.17 and 0.50 ng·mL -1 , respectively. The extraction recoveries for MTZ and DZP were found to be between 84.9 and 93.9%. The intra-day and inter-day precision and accuracy fulfill at the international acceptance criteria. The method shows to be stable for the studied parameters. Therefore, a rapid, specific, and sensitive LC-MS/MS method for quantification of MTZ in human plasma was developed and can be used in therapeutic drug monitoring of this drug.

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Section: Introductionmentioning

confidence: 99%