Ozone therapy ameliorates paraquat-induced lung injury in rats

Abstract

Paraquat (PQ) overdose can cause acute lung injury and death. Ozone therapy (OT) was previously demonstrated to alleviate inflammation and necrosis in various pathologies. We therefore hypothesized that OT has ameliorative and preventive effects on PQ-induced lung damage due to anti-inflammatory and antioxidants properties. Sprague-Dawley rats (n = 24) were separated into three groups: sham, PQ, and PQ+OT groups. 15 mg/kg PQ was administered intraperitoneally in PQ and PQ+OT groups to induce experimental lung injury. One hour after PQ treatment, PQ+OT group was administered a single dose of ozone-oxygen mixture (1 mg/kg/day) by intraperitoneal route for four consecutive days. The animals were sacrificed on fifth day after PQ administration. Blood samples and lung tissues were collected to evaluate the inflammatory processes, antioxidant defense and pulmonary damage. Serum lactate dehydrogenase (LDH) and neopterin levels, tissue oxidative stress parameters, total TGF-β1 levels, and histological injury scores in PQ+OT group were significantly lower than PQ group (P<0.05, PQ vs. PQ+OT). Total antioxidant capacity in PQ+OT group was significantly higher than PQ group (P < 0.05, PQ+OT vs. PQ). These findings suggest that outcome in PQ-induced lung injury may be improved by using OT as an adjuvant therapy.

Embelin impact on paraquat‐induced lung injury through suppressing oxidative stress, inflammatory cascade, and MAPK/NF‐κB signaling pathway

“…The binding of specific antibody was imagined by presenting the layers to photographic film after treatment with improved chemiluminescence framework reagents, and the protein expression was measured using image analyzing software (Image Pro software, Ver 10) from the Media Cybernetics Inc (Rockville, MD). [ 22,23 ]…”

Potential Cytoprotective Activity of Ozone Therapy in SARS-CoV-2/COVID-19

“…OT stabilizes hepatic metabolism and tend to normalize fibrinogen and prothrombin plasma levels in infected patients, suggesting an improvement of the hepatic protein synthesis [15]. There is extensive research demonstrating the cytoprotective effect of ozone to prevent oxidative damage to the heart [44,45], liver [46,47], lungs [48] and renal tissues [49]. The authors have described in a recent review, the cytoprotective effect of ozone to prevent and even to treat chemotherapy-induced damage in heart, liver, renal and lung tissue [22].…”

Clinical effectiveness of medical ozone therapy in COVID-19: the evidence and gaps map

“…There is also a demonstration that OT protects against oxidative damage to the lung,[ 25 ] kidney,[ 26 ] heart,[ 27 ] intestine,[ 28 ] muscles[ 29 ] and brain. [ 30 ]…”