Ozone risk and foliar injury on Viburnum lantana L.: A meso-scale epidemiological study

Gottardini, E.; Cristofolini, F.; Cristofori, Alessandro; Ferretti, Marco

doi:10.1016/j.scitotenv.2014.06.041

Cited by 11 publications

(9 citation statements)

References 35 publications

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“…Visible foliar injury has been used in many field experiments as an indicator of the response of V. lantana to O 3 exposure (e.g., Gottardini et al, 2010 ). Fully expanded leaves showed symptoms similar to those previously reported in seedlings from OTC experiments (Novak et al, 2005 , 2008 ) and in plants grown in natural conditions (Gottardini et al, 2014a , b ).…”

Section: Resultssupporting

confidence: 86%

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Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

et al. 2016

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Tropospheric ozone (O3) is a global air pollutant that causes high economic damages by decreasing plant productivity. It enters the leaves through the stomata, generates reactive oxygen species, which subsequent decrease in photosynthesis, plant growth, and biomass accumulation. In order to identify genes that are important for conferring O3 tolerance or sensitivity to plants, a suppression subtractive hybridization analysis was performed on the very sensitive woody shrub, Viburnum lantana, exposed to chronic O3 treatment (60 ppb, 5 h d−1 for 45 consecutive days). Transcript profiling and relative expression assessment were carried out in asymptomatic leaves, after 15 days of O3 exposure. At the end of the experiment symptoms were observed on all treated leaves and plants, with an injured leaf area per plant accounting for 16.7% of the total surface. Cloned genes were sequenced by 454-pyrosequencing and transcript profiling and relative expression assessment were carried out on sequenced reads. A total of 38,800 and 12,495 high quality reads obtained in control and O3-treated libraries, respectively (average length of 319 ± 156.7 and 255 ± 107.4 bp). The Ensembl transcriptome yielded a total of 1241 unigenes with a total sequence length of 389,126 bp and an average length size of 389 bp (guanine-cytosine content = 49.9%). mRNA abundance was measured by reads per kilobase per million and 41 and 37 ensembl unigenes showed up- and down-regulation respectively. Unigenes functionally associated to photosynthesis and carbon utilization were repressed, demonstrating the deleterious effect of O3 exposure. Unigenes functionally associated to heat-shock proteins and glutathione were concurrently induced, suggesting the role of thylakoid-localized proteins and antioxidant-detoxification pathways as an effective strategy for responding to O3. Gene Ontology analysis documented a differential expression of co-regulated transcripts for several functional categories, including specific transcription factors (MYB and WRKY). This study demonstrates that a complex sequence of events takes place in the cells at intracellular and membrane level following O3 exposure and elucidates the effects of this oxidative stress on the transcriptional machinery of the non-model plant species V. lantana, with the final aim to provide the molecular supportive knowledge for the use of this plant as O3-bioindicator.

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Section: Resultssupporting

confidence: 86%

“…Wayfaring tree sensitivity has been already assessed in terms of morphological (i.e. foliar symptoms) and physiolgical traits in order to evaluate its potential as bio-indicator (Gottardini et al, 2010 , 2014a , b ). Although a series of studies has been conducted on Viburnum spp.…”

Section: Introductionmentioning

confidence: 99%

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

et al. 2016

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“…Necrosis started from the peripheral leaf blade toward the central vein, and covered almost the entire interveinal spaces with progressing stress. These injuries are typical O 3 -induced foliar visible injuries, which have been observed in various plant species (Hoshika et al 2013;Feng et al 2014;Wan et al 2014;Gottardini et al 2014). The occurrence of foliar visible injuries, with decreased greenness, indicates that the shrubs grown in EOZ were under considerable O 3 -induced stress from the first growing season.…”

Section: Ozone Effects On Plant Physiology In the First Growing Season (2014)mentioning

confidence: 74%

Ethylenediurea (EDU) spray effects on willows (Salix sachalinensis F. Schmid) grown in ambient or ozone-enriched air: implications for renewable biomass production

et al. 2021

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Ground-level ozone (O3) is a widespread air pollutant causing extensive injuries in plants. However, its effects on perennial energy crops remain poorly understood due to technical difficulties in cultivating fast-growing shrubs for biomass production under O3 treatment on the field. Here we present the results of a two-year evaluation in the framework of which willow (Salix sachalinensis F. Schmid) shrubs were exposed to ambient (AOZ) or elevated (EOZ) O3 in two successive growing seasons (2014, 2015) and treated with 0 (EDU0) or 400 mg L−1 (EDU400) ethylenediurea spray in the second growing season. In 2014, EOZ altered the chemical composition of both top young and fallen leaves, and a novel mechanism of decreasing Mg in fallen leaves while highly enriching it in young top leaves was revealed in shrubs exposed to EOZ. In 2015, EDU400 alleviated EOZ-induced decreases in leaf fresh mass to dry mass ratio (FM/DM) and leaf mass per area (LMA). While EDU400 protected against EOZ-induced suppression of the maximum rate at which leaves can fix carbon (Amax) in O3-asymptomatic leaves, it did not alleviate EOZ-induced suppression of the maximum rates of carboxylation (VCmax) and electron transport (Jmax) and chlorophylls a, b, and a + b in the same type of leaves. In O3-symptomatic leaves, however, EDU400 alleviated EOZ-induced suppression of chlorophylls a and a + b, indicating different mode of action of EDU between O3-asymptomatic and O3-symptomatic leaves. Extensive herbivory occurred only in AOZ-exposed plants, leading to suppressed biomass production, while EOZ also led to a similar suppression of biomass production (EDU0 × EOZ vs. EDU400 × EOZ). In 2016, carry-over effects were also evaluated following cropping and transplantation into new ambient plots. Effects of EOZ in the preceding growing seasons extended to the third growing season in the form of suppressed ratoon biomass production, indicating carry-over effect of EOZ. Although EDU400 protected against EOZ-induced suppression of biomass production when applied in 2015, there was no carry-over effect of EDU in the absence of EDU treatment in 2016. The results of this study provide novel mechanistic understandings of O3 and EDU modes of action and can enlighten cultivation of willow as energy crop.

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“…Previous in-field studies showed that the frequency of ozone VFS on native V . lantana plants is consistent with the ozone exposure levels within the same growing season [ 13 ], over distinct growing seasons [ 18 ] and even at the meso-scale spatial resolution [ 19 ].…”

Section: Introductionmentioning

confidence: 54%

Leaf trait plasticity and site-specific environmental variability modulate the severity of visible foliar ozone symptoms in Viburnum lantana

et al. 2022

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The assessment of Visible Foliar Symptoms (VFS) is commonly adopted by forest monitoring programs to evaluate ozone impact on vegetation. The occurrence of ozone VFS may differ among individuals of the same species at the same site, and within leaves of the same individual. The aim of this study was to identify site and plant characteristics as well as functional leaf traits associated with the occurrence and severity of VFS in Viburnum lantana (an ozone-sensitive species) and at the scale of an individual site. V. lantana plants growing at one site of the ViburNeT monitoring network (Trentino, North Italy) experiencing high ozone levels were surveyed in relation to 1) sun exposure, 2) shading effect from neighbor vegetation, 3) plant height and 4) presence and severity of VFS. Leaves from three different sections of each plant were subjected to a phenotypic characterization of leaf area, dry weight, specific leaf area (SLA), chlorophyll content (ChlSPAD), percentage of VFS, and adaxial and abaxial trichome density (Tr). We showed that plants at high irradiation levels had significantly lower SLA (p<0.05), higher Tr (p<0.01) and greater ChlSPAD (p<0.01) when compared to shaded and/or west- and north-exposed plants, thus indicating a strong influence of site-specific characteristics on leaf trait plasticity. Similar differences were observed for taller vs. shorter plants and apical vs. basal branches (p<0.05). Ozone-induced VFS at leaf level were associated with lower SLA (p<0.001) and higher Tr in the abaxial leaf surface (p<0.05). Both leaf traits showed significant differences also within the south and east exposed plant category, thus suggesting the increase in leaf thickness and Tr as a potential adaptive strategy under multiple stress conditions. Our results provide evidence of a strong relationship between VFS, leaf traits and site-specific variables, offering new insights for interpreting data on the impact of ozone on vegetation.

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Ozone risk and foliar injury on Viburnum lantana L.: A meso-scale epidemiological study

Cited by 11 publications

References 35 publications

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

Suppression Substractive Hybridization and NGS Reveal Differential Transcriptome Expression Profiles in Wayfaring Tree (Viburnum lantana L.) Treated with Ozone

Ethylenediurea (EDU) spray effects on willows (Salix sachalinensis F. Schmid) grown in ambient or ozone-enriched air: implications for renewable biomass production

Leaf trait plasticity and site-specific environmental variability modulate the severity of visible foliar ozone symptoms in Viburnum lantana

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