Ozone Partially Prevents Diabetic Neuropathy in Rats

Erken, Haydar Ali; Genç, Osman; Erken, Gülten; Ayada, Ceylan; Gündoğdu, Gülşah; Doğan, Hayriye Tatlı

doi:10.1055/s-0034-1389954

Cited by 15 publications

(16 citation statements)

References 28 publications

(39 reference statements)

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“…Erken et al demonstrated that the medical ozone therapy given by rectal insufflation reduces TOS and OSI levels and partially prevents diabetic neuropathy in rats [29]. In line with this study, we noticed renoprotective effects by MOA as reflected by decrements in TOS and OSI levels.…”

Section: Discussionsupporting

confidence: 91%

Major Ozonated Autohemotherapy Preconditioning Ameliorates Kidney Ischemia-Reperfusion Injury

et al. 2015

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Medical ozone has therapeutic properties as an antimicrobial, anti-inflammatory, modulator of antioxidant defense system. Major ozonated autohemotherapy (MOA) is a new therapeutic approach that is widely used in the treatment of many diseases. The objective of the present study was to determine whether preischemic application of MOA would attenuate renal ischemia-reperfusion injury (IRI) in rabbits. Twenty-four male New Zealand white rabbits were divided into four groups, each including six animals: (1) Sham-operated group, (2) Ozone group (the MOA group without IRI), (3) IR group (60 min ischemia followed by 24 h reperfusion), and (4) IR + MOA group (MOA group). The effects of MOA were examined by use of hematologic and biochemical parameters consisting of neutrophil to lymphocyte ratio (NLR), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), ischemia-modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI). In addition, the histopathological changes including the tubular brush border loss (TBBL), tubular cast (TC), tubular necrosis (TN), intertubular hemorrhage and congestion (IHC), dilatation of bowman space (DBS), and interstitial inflammatory cells infiltration (IECI) were evaluated. In the IR group, compared to the Sham group, biochemical parameters indicating oxidative stress, NLR, IL-6, TNF-α, IMA, TOS, and OSI have increased. MOA reduced inflammation and oxidative stress parameters. Although TAS values have decreased in the IR group and increased in the MOA-pretreated group, no significant changes in TAS values were detected between the IR and MOA groups. The total score was obtained by summing all the scores from morphological kidney damage markers. The total score has increased with IR damage when compared with the Sham group (13.83 ± 4.30 vs 1.51 ± 1.71; p = 0.002). But, the total score has decreased significantly after application of MOA (5.01 ± 1.49; p = 0.002; compared with the IR group). MOA preconditioning is effective in reducing tissue damage induced in kidney ischemia-reperfusion injury. The protective effect of MOA is mediated via reducing inflammatory response and regulating of reactive oxygen species (ROS). Renal histology also showed convincing evidence regarding MOA's protective nature against kidney injury induced renal ischemia-reperfusion. Consequently, MOA might be helpful in protecting the kidneys from IR-induced damage in humans, probably through the anti-inflammatory effect and reducing the total oxidant status.

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Section: Discussionsupporting

confidence: 91%

Major Ozonated Autohemotherapy Preconditioning Ameliorates Kidney Ischemia-Reperfusion Injury

et al. 2015

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“…The incidence of chronic limb ischemia, which is habitually comorbidity in type 2 diabetes has been shown to be reduced with ozone therapy (Bocci, ; Bocci et al, ). Moreover, ozone therapy partially prevented diabetic neuropathy in rats 4 weeks after induction of diabetes following a 15‐day exposure to ozone (1.1 mg/kg), through oxidant/antioxidant mechanisms (Erken et al, ).…”

Section: Ozone Therapy To Attenuate Oxidative Stress In Diabetesmentioning

confidence: 99%

Therapeutic relevance of ozone therapy in degenerative diseases: Focus on diabetes and spinal pain

Braidy

Izadi

Sureda

et al. 2017

Journal Cellular Physiology

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Ozone, one of the most important air pollutants, is a triatomic molecule containing three atoms of oxygen that results in an unstable form due to its mesomeric structure. It has been well-known that ozone has potent ability to oxidize organic compounds and can induce respiratory irritation. Although ozone has deleterious effects, many therapeutic effects have also been suggested. Since last few decades, the therapeutic potential of ozone has gained much attention through its strong capacity to induce controlled and moderated oxidative stress when administered in precise therapeutic doses. A plethora of scientific evidence showed that the activation of hypoxia inducible factor-1α (HIF-1a), nuclear factor of activated T-cells (NFAT), nuclear factor-erythroid 2-related factor 2-antioxidant response element (Nrf2-ARE), and activated protein-1 (AP-1) pathways are the main molecular mechanisms underlying the therapeutic effects of ozone therapy. Activation of these molecular pathways leads to up-regulation of endogenous antioxidant systems, activation of immune functions as well as suppression of inflammatory processes, which is important for correcting oxidative stress in diabetes and spinal pain. The present study intended to review critically the available scientific evidence concerning the beneficial properties of ozone therapy for treatment of diabetic complications and spinal pain. It finds benefit for integrating the therapy with ozone into pharmacological procedures, instead of a substitutive or additional option to therapy.

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“…Therefore, this study aimed to investigate the effectiveness of systemic O 3 treatment in an animal model for RA. Previous studies have shown that systemic O 3 administration could be used safely in some clinical conditions, such as diabetes mellitus and renal disorders, 18,19 but a literature review indicates that this study is the first to evaluate the effect of systemic O 3 on both clinical and histopathological findings in an animal model of RA.…”

Section: Discussionmentioning

confidence: 95%

Effect of Ozone in Freund’s Complete Adjuvant-Induced Arthritis

et al. 2018

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Objectives: This study aims to investigate the effectiveness and reliability of ozone (O3) in Freund's complete adjuvant (FCA)-induced arthritis, an animal model for rheumatoid arthritis (RA). Materials and methods: Thirty-six four-to five-month-old male Wistar rats weighing between 274-420 gr were used in this study. Saline was injected into the hind paws of half of these rats, and FCA was injected into the other half. At the end of two weeks, 40 µg of O3 was administered to nine rats from each group twice a week for seven total doses. The rats were followed-up in terms of clinical findings. At the sixth week, the rats were sacrificed and serum malondialdehyde, glutathione peroxidase, and superoxide dismutase levels were measured. In addition, ankle joints were separated for histopathological examination. Results: Significant improvement was observed in terms of hind-paw diameter, severity of arthritis, and histopathological findings of inflammation after O3 treatment in the group with FCA-induced arthritis. Although it was not quite significant, an upward trend was detected in oxidative stress markers with O3 treatment. Conclusion: This study, the first to investigate the effects of systemic O3 on the clinical and histopathological outcomes of RA, indicates that O3 is a highly effective and reliable treatment method in FCA-induced arthritis in animal models.

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Ozone Partially Prevents Diabetic Neuropathy in Rats

Cited by 15 publications

References 28 publications

Major Ozonated Autohemotherapy Preconditioning Ameliorates Kidney Ischemia-Reperfusion Injury

Major Ozonated Autohemotherapy Preconditioning Ameliorates Kidney Ischemia-Reperfusion Injury

Therapeutic relevance of ozone therapy in degenerative diseases: Focus on diabetes and spinal pain

Effect of Ozone in Freund’s Complete Adjuvant-Induced Arthritis

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