Abstract:PURPOSE:To investigate the protective effects of ozone oxidative preconditioning (OzoneOP) were associated with the modulation of TLR4-NF-κB pathway.
METHODS:Thirty six rats were subjected to 45 min of renal ischemia, with or without treatment with OzoneOP (1 mg/kg). Blood samples were collected for the detection of blood urea nitrogen and creatinine levels. Histologic examinations were evaluated and immunohistochemistry was also performed for localization of TLR4 and NF-κB. The expression of TNF-α, IL-1β, IL-… Show more
“…39 Ameliorative effect of OT on oxidative stress in our study is also in agreement with those displayed in researches related with ischemia/reperfusion injury in kidney. 37,38,40,41 In conclusion,…”
Section: Discussionmentioning
confidence: 74%
“…It has also been shown that ozone administration stimulates oxidative preconditioning or enhances adaptation to oxidative stress involving glutathione, SOD, and catalase and enzymatic reactions, preparing the host to face physiopathological conditions mediated by ROS/ RNS. 37,38 In light of recent pharmacological knowledge, our consideration is that ozone acts as a pro-drug and induces a rearrangement of the biochemical pathways with the activation of a second messenger in a cascade with a multiple system action. 39 Ameliorative effect of OT on oxidative stress in our study is also in agreement with those displayed in researches related with ischemia/reperfusion injury in kidney.…”
Objectives: Extracorporeal shock wave (ESW) lithotripsy is the preferred treatment modality for uncomplicated kidney stones. More recently free oxygen radical production following ESW application has been considered to be crucial in shock wave-induced renal damage. It has been shown that ozone therapy (OT) has ameliorative and preventive effects against various pathological conditions due to increased nitro-oxidative stress. In current study, we aimed to evaluate the efficacy of OT against ESW-induced renal injury. Methods: Twenty-four male Sprague-Dawley rats were divided into three groups: sham-operated, ESW, and ESW þ OT groups. All groups except shamoperated group were subjected to ESW procedure. ESW þ OT group received 1 mg/kg/day of oxygen/ozone mixture intraperitoneally at 2 h before ESW, and OT was continued once a day for consecutive three days. The animals were killed at the 4th day, and kidney tissue and blood samples were harvested for biochemical and histopathologic analysis. Results: Serum ALT and AST levels, serum neopterin, tissue nitrite/nitrate levels, and tissue oxidative stress parameters were increased in the ESW group and almost came close to control values in the treatment group (p < 0.05, ESW vs. ESW þ OT). Histopathological injury scores were significantly lower in treatment group than the ESW group (p < 0.05, ESW vs. ESW þ OT). Immunohistochemical iNOS staining scores in ESW group were higher than those of sham-operated group (p < 0.05, ESW vs. sham-operated), iNOS staining scores in OT group were significantly lower than the ESW group (p < 0.05, ESW þ OT vs. ESW). Conclusion: OT ameliorates nitro-oxidative stress and reduces the severity of pathological changes in the experimental ESW-induced renal injury of rat model.
ARTICLE HISTORY
“…39 Ameliorative effect of OT on oxidative stress in our study is also in agreement with those displayed in researches related with ischemia/reperfusion injury in kidney. 37,38,40,41 In conclusion,…”
Section: Discussionmentioning
confidence: 74%
“…It has also been shown that ozone administration stimulates oxidative preconditioning or enhances adaptation to oxidative stress involving glutathione, SOD, and catalase and enzymatic reactions, preparing the host to face physiopathological conditions mediated by ROS/ RNS. 37,38 In light of recent pharmacological knowledge, our consideration is that ozone acts as a pro-drug and induces a rearrangement of the biochemical pathways with the activation of a second messenger in a cascade with a multiple system action. 39 Ameliorative effect of OT on oxidative stress in our study is also in agreement with those displayed in researches related with ischemia/reperfusion injury in kidney.…”
Objectives: Extracorporeal shock wave (ESW) lithotripsy is the preferred treatment modality for uncomplicated kidney stones. More recently free oxygen radical production following ESW application has been considered to be crucial in shock wave-induced renal damage. It has been shown that ozone therapy (OT) has ameliorative and preventive effects against various pathological conditions due to increased nitro-oxidative stress. In current study, we aimed to evaluate the efficacy of OT against ESW-induced renal injury. Methods: Twenty-four male Sprague-Dawley rats were divided into three groups: sham-operated, ESW, and ESW þ OT groups. All groups except shamoperated group were subjected to ESW procedure. ESW þ OT group received 1 mg/kg/day of oxygen/ozone mixture intraperitoneally at 2 h before ESW, and OT was continued once a day for consecutive three days. The animals were killed at the 4th day, and kidney tissue and blood samples were harvested for biochemical and histopathologic analysis. Results: Serum ALT and AST levels, serum neopterin, tissue nitrite/nitrate levels, and tissue oxidative stress parameters were increased in the ESW group and almost came close to control values in the treatment group (p < 0.05, ESW vs. ESW þ OT). Histopathological injury scores were significantly lower in treatment group than the ESW group (p < 0.05, ESW vs. ESW þ OT). Immunohistochemical iNOS staining scores in ESW group were higher than those of sham-operated group (p < 0.05, ESW vs. sham-operated), iNOS staining scores in OT group were significantly lower than the ESW group (p < 0.05, ESW þ OT vs. ESW). Conclusion: OT ameliorates nitro-oxidative stress and reduces the severity of pathological changes in the experimental ESW-induced renal injury of rat model.
ARTICLE HISTORY
“…Furthermore, ozone therapy was reported to limit the effects of experimentally induced I/R injury in multiple organs (24,25). Xing et al (26) reported that tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, in- MWM; Morris water maze, PFT; platform fi nding time, data given are mean ± SD, P 0 : Indicates the difference in PFT from day 1 to day 4 within each group, P 1 , P 2 , P 3 , P 4 , P 5 , and P 6 indicates the difference in PFT between the sham and control groups, between the sham and ozone1 groups, between the sham and ozone2 groups, between the control and ozone1 groups, between the control and ozone2 groups, and between the ozone1 and ozone2 groups, respectively…”
Section: Discussionmentioning
confidence: 99%
“…Based on these fi ndings, Xing et al (26) suggested that ozone OP exerted a potent anti-infl ammatory effect via modulation of TLR4 and NF-kappa. Wang et al (6) reported a signifi cant decrease in renal fi brosis in response to ozone OP in rats with experimentally induced renal I/R.…”
OBJECTIVES: This study is aimed to determine the effect of ozone therapy in neonatal rats with experimentally induced hypoxic ischemic brain injury (HIBI). METHODS: The study included 7-d-old male Wistar rats that were randomized to the sham, control, ozone 1, and ozone 2 groups. All rats except those in the sham group were kept in a hypoxia chamber, and then the rats in the control group were given 0.5 mL of saline. Those in the ozone 1 group were given ozone 1 mg kg -1 intraperitoneally, and those in the ozone 2 group were given ozone 2 mg kg -1 intraperitoneally. RESULTS: There were signifi cantly fewer apoptotic neurons in the right hemispheres of the rats in the ozone 1 and ozone 2 groups than in the control group (p < 0.001 and p < 0.001, respectively). There were signifi cantly fewer apoptotic neurons in the right hemispheres of the rats in the ozone 2 group than in the ozone 1 group (p < 0.001). Morris Water Maze (MWM) test results were similar in the ozone 2 and sham groups. CONCLUSIONS: The present study's fi ndings show that ozone therapy reduced neuronal apoptosis and improved cognitive function in neonatal rats with experimentally induced HIBI (Tab. 2, Ref. 30). Text in PDF www.elis.sk.
“…[12][13][14] Lately the anti-inflammation action of this magical treatment has been recognized by some researchers in animal experiments and the mechanisms involved in may associate with the mediation of TLR4. [15][16][17][18] However, whether ozone therapy could also be a promising strategy ameliorating chronic tubuleinterstitial inflammation in CKD patients or CKD animal models has not been discussed yet. Therefore, this paper created a rat model of CKD and aimed at investigating whether ozone therapy could ameliorate tubulointerstitial inflammation by the regulation of TLR4.…”
Tubulointerstitium inflammation is a common pathway aggravating chronic kidney disease (CKD) progression and the mechanism is partly associated with excessive activation of toll-like receptor 4 (TLR4) in tubulointerstitium. Ozone therapy is demonstrated to alleviate inflammation in some experiments. The aim of this study is to examine whether ozone therapy could ameliorate chronic tubulointerstitium inflammation by suppressing TLR4 in adenine-induced CKD rats. SpragueDawley rats were fed with 0.75% adenine-containing diet to induce CKD and tubulointerstitium inflammation injury. Ozone therapy (1.1 mg/kg) was simultaneously administrated by rectal insufflations (i.r.). After 4 weeks, serum and kidney samples were collected for detection. Renal function and systemic electrolyte were detected. Renal pathological changes were assessed by hematoxylin-eosin (H&E) staining and Masson trichrome (MT) staining. Immunohistochemistry, Western blot and Real-time PCR were applied to evaluate tubulointerstitium inflammation as well as the expression of TLR4 and phosphorylated nuclear factor kappa B P65 (p-NF-kB P65) in rats. The results showed ozone therapy improved serious renal insufficiency, systemic electrolyte disorder and tubulointerstitium morphology damages in adenine-induced CKD rats. In addition, ozone therapy suppressed excessive activation of TLR4 and p-NF-kB P65 in the tubulointerstitium of adenine-induced CKD rats, accompanied by the reduction of inflammation-related cytokines including monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-a (TNF-a), interleukin-1b (IL-1b) and interleukin-6 (IL-6). The protein expression of TLR4 was positively correlated with the protein expression levels of MCP-1 (r ¼ 0.7863, p50.01) and TNF-a (r ¼ 0.7547, p50.01) in CKD rats. These findings indicated ozone therapy could attenuate tubulointerstitium inflammation injury in adenine-induced CKD rats and the mechanism might associate with the mediation of TLR4.
ARTICLE HISTORY
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