Ozone oxidative preconditioning: a protection against cellular damage by free radicals

Abstract

There is some anecdotal evidence that oxygen-ozone therapy may be beneficial in some human diseases. However so far only a few biochemical and pharmacodynamic mechanisms have been elucidated. On the basis of preliminary data we postulated that controlled ozone administration would promote an oxidative preconditioning preventing the hepatocellular damage mediated by free radicals. Six groups of rats were classified as follows: (1) negative control, using intraperitoneal sunflower oil; (2) positive control using carbon tetrachloride (CCl4) as an oxidative challenge; (3) oxygen-ozone, pretreatment via rectal insufflation (15 sessions) and after it, CCl4; (4) oxygen, as group 3 but using oxygen only; (5) control oxygen-ozone, as group 3, but without CCl4; group (6) control oxygen, as group 5, but using oxygen only. We have evaluated critical biochemical parameters such as levels of transaminase, cholinesterase, superoxide dismutase, catalase, phospholipase A, calcium dependent ATPase, reduced glutathione, glucose 6 phosphate dehydrogenase and lipid peroxidation. Interestingly, in spite of CCl4 administration, group 3 did not differ from group 1, while groups 2 and 4 showed significant differences from groups 1 and 3 and displayed hepatic damage. To our knowledge these are the first experimental results showing that repeated administration of ozone in atoxic doses is able to induce an adaptation to oxidative stress thus enabling the animals to maintain hepatocellular integrity after CCl4 poisoning.

Effect of ozone on colon anastomoses in rat peritonitis model

“…A controlled number and dose of ozone treatments conferred protection against different physiopathological processes mediated by ROS was reported 16 .…”

Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

“…The ozone dose is the product of the O3 concentration (expressed as mg/l) by the gas (O2 + O3 ) volume (l). By knowing the body weight of the rat the O3 dose is calculated as 1 mg/kg 8) .…”

Efecto del sulfato de cobre en el daño pulmonar inducido por la exposición crónica intermitente a ozono

“…La inhalación de oxigeno + ozono por 10 o 15 días produce pre-acondicionamiento al estrés oxidativo por ozono y protege al hígado del daño por isquemia y reperfusión, que se ha explicado por aumento de la expresión de enzimas antioxidantes (superóxido dismutasa, catalasa y glutatión peroxidasa), que probablemente gatillen el pre-acondicionamiento oxidativo por ozono [36][37][38] . Por otra parte, en simulaciones de shock endotóxico, el ozono protege a la rata disminuyendo TNF-α sanguíneo, esto se explicaría porque los antioxidantes generados por el ozono, administrado antes de la lesión, disminuyen el NF-kB y las citoquinas circulantes 39,40 .…”