Ozone nanobubble treatment in freshwater effectively reduced pathogenic fish bacteria and is safe for Nile tilapia (Oreochromis niloticus)

Jhunkeaw, Chayuda; Khongcharoen, Nareerat; Rungrueng, Naruporn; Sangpo, Pattiya; Panphut, Wattana; Thapinta, Darawan; Senapin, Saengchan; St‐Hilaire, Sophie; Dong, Ha Thanh

doi:10.1016/j.aquaculture.2020.736286

Cited by 46 publications

(26 citation statements)

References 19 publications

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“…Toxicity resulting in mortalities was reported for experimental shrimp in a study by Imaizumi et al (2018). In our previous study on tilapia, we did not observe fish mortality, but the fish gill morphology was damaged when fish were exposed directly to multiple NB-O 3 treatments with an ORP range between 860 ± 42 and 885 ± 15 mV (Jhunkeaw et al, 2021). In this study, we set up a modified recirculation system coupled with ozone nanobubbles (MRS-NB-O 3 ).…”

Section: Introductionmentioning

confidence: 84%

“…Several studies have reported potential applications of NB‐O 3 for pathogen disinfection in aquaculture water to reduce the risk of infectious diseases in both fish and shrimp (Imaizumi et al., 2018; Jhunkeaw et al., 2021; Kurita et al., 2017). We recently reported an additional benefit of NB‐O 3 in modulating of the innate immune defence system in Nile tilapia to fight against S. agalactiae (Linh et al., 2021).…”

Section: Discussionmentioning

confidence: 99%

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Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Dien

Linh

Sangpo

et al. 2021

Journal of Fish Diseases

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A rapid increase in multi‐drug‐resistant (MDR) bacteria in aquaculture highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB‐O3) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple treatments with direct NB‐O3 exposures caused alterations to the gills of exposed fish. Here, we set up a modified recirculation system (MRS) assembled with an NB‐O3 device (MRS‐NB‐O3) to investigate whether MRS‐NB‐O3 (a) were safe for tilapia (Oreochromis niloticus), (b) were effective at reducing bacterial load in rearing water and (c) improved survivability of Nile tilapia following an immersion challenge with a lethal dose of MDR Aeromonas hydrophila. The results showed no behavioural abnormalities or mortality of Nile tilapia during the 14‐day study using the MRS‐NB‐O3 system. In the immersion challenge, although high bacterial concentration (~2 × 107 CFU/ml) was used, multiple NB‐O3 treatments in the first two days reduced the bacteria between 15.9% and 35.6% of bacterial load in water, while bacterial concentration increased from 13.1% to 27.9% in the untreated control. There was slight up‐regulation of non‐specific immune‐related genes in the gills of the fish receiving NB‐O3 treatments. Most importantly, this treatment significantly improved survivability of Nile tilapia with relative percentage survival (RPS) of 64.7% – 66.7% in treated fish and surviving fish developed specific antibody against MDR A. hydrophila. In summary, the result suggests that NB‐O3 is a promising non‐antibiotic approach to control bacterial diseases, including MDR bacteria, and has high potential for application in recirculation aquaculture system (RAS).

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Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Dien

Linh

Sangpo

et al. 2021

Journal of Fish Diseases

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“…Ozone is also considered attractive for veterinary and food applications, which is important in the new era of the holistic "one health" view [40,41].…”

Section: Discussionmentioning

confidence: 99%

Ozone Gel in Chronic Periodontal Disease: A Randomized Clinical Trial on the Anti-Inflammatory Effects of Ozone Application

Colombo

Gallo

Garofoli

et al. 2021

Biology

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The search for new topical treatments able to display not only antimicrobial properties but also a multiplicity of other beneficial effects while expressing safe cytocompatibility toward host tissues is being progressively developed. Antiseptics represent an aid to the gold standard nonsurgical treatment Scaling-and-Root-Planing (SRP) for periodontal disease. This split-mouth study aims to assess the efficacy of the ozonized gel GeliO3 (Bioemmei Srl, Vicenza, Italy) plus SRP (experimental treatment), with respect to SRP + chlorhexidine gel. Ten participants were treated with SRP + chlorhexidine gel (control sites) and with SRP + ozone gel (trial sites). After 1 (T1) and 3 months (T2) from baseline (T0), patients were revisited. At each time-point, the following indexes were assessed: probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI), plaque index (PI), and bleeding on probing (BoP). It has been assessed that the use of the ozonized gel in addition to SRP did not show significant differences if compared to conventional SRP + chlorhexidine. Chlorhexidine was found to be more effective than ozone in reducing CAL and GI at T2. Ozone deserves consideration for its wide applicability in several clinical fields. In this connection, we also glance at the latest research on ozone therapy.

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“…Ozone gas nanobubble (NB-O3) technology is not only a beneficial disinfection method, but also supplies dissolved oxygen (DO) in freshwater aquaculture, and it is not harmful to the fish in low dosage. Jhunkeaw et al (2021) reported that upon NB-O3 treatment, the number of bacterial colonies reduced rapidly during 10 min following three times continuous exposure in the treated tanks. Before the treatment, the total bacterial density in the fish-cultured water was 6.93 × 10 5 ± 7.81 × 10 5 CFU/mL and 42.94% of the bacteria were inactivated after the NB-O3 exposure for 10 min.…”

Section: Ozone Nanobubble Treatmentmentioning

confidence: 98%

Common Bacterial Diseases of Fish: Prevention and Control Strategies

2021

Int J Vet Sci

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Ozone nanobubble treatment in freshwater effectively reduced pathogenic fish bacteria and is safe for Nile tilapia (Oreochromis niloticus)

Cited by 46 publications

References 19 publications

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Ozone nanobubble treatments improve survivability of Nile tilapia (Oreochromis niloticus) challenged with a pathogenic multi‐drug‐resistant Aeromonas hydrophila

Ozone Gel in Chronic Periodontal Disease: A Randomized Clinical Trial on the Anti-Inflammatory Effects of Ozone Application

Common Bacterial Diseases of Fish: Prevention and Control Strategies

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