Ozone-induced oxidative modification of plasma fibrin-stabilizing factor

Ozone-induced oxidative modification of fibrinogen: Role of the D regions

“…Therefore, the fraction of ozone experiencing decomposition via free radical oxidation of fibrinogen may be significantly higher. As was calculated earlier [25], an approximate order of generation of free radicals in 1 ml of blood serum may be in range of 3 Â 10 À 10 to 6 Â 10 À 10 mol/min. Fibrinogen is known to be 20 times more sensitive to oxidative modification than other major plasma proteins (albumin, immunoglobulins, transferrin, and ceruloplasmin) [23].…”

“…First, under these conditions, neither formation of intermolecular covalent crosslinks nor intrachain fragmentation should occur. Second, the assessment made earlier indicated a probable relative similarity of the oxidant levels used in the model system for oxidation of fibrinogen and those ROS levels existing in blood plasma under physiological conditions [25].…”

“…Oxidation of fibrinogen was initiated by ozone [25]. The oxidizer was chosen from the following considerations.…”

Nature of active intermediate particles formed during ozone-induced oxidation

“…It was previously shown that a number of proteins (fibrinogen [5,6], fibrin stabilizing factor [7], bovine serum albumin [8], etc.) are involved in oxidative pro cesses under the influence of ozone.…”

The oxidative modification of cellular fibrin-stabilizing factor

“…It is also believed that subunit B can serve as an inhibitor of subunit A activa tion in blood plasma [6]. We have previously assumed that the regulatory subunits may be antioxidant struc tural elements of pFXIII [7].…”