Ozone high dose therapy (OHT) improves mitochondrial bioenergetics in peripheral blood mononuclear cells

Abstract

Background The worldwide increasing number of people with chronic diseases is pushing conventional therapy to its limits. The so-called Major AutoHaemo Therapy (MAH) has been used in many practices for years. Despite suspicions, especially the 10-passes ozone-high-dosis Therapy (OHT) has shown substantial benefits in chronic ailments. However, knowledge of scientifically based effects of high ozone concentrations are still rare. The present investigation focussed on verifying whether OHT may be linked to a beneficial effect on mitochondrial bioenergetics which can be expressed as a bioenergetic health index (BHI). Methods We report on six patients which received OHT for preventive purposes twice within one week. The BHI in peripheral blood mononuclear cells (PBMC) is calculated from parameters of a cellular mitochondrial function assay, which gives insights into different aspects of mitochondrial function: 1) Basal oxygen consumption rate (OCR); 2) ATP-linked OCR and proton leak; 3) Maximal OCR and reserve capacity; 4) Non-mitochondrial OCR. Results The results clearly show that the bioenergetic health index in PBMC improves significantly after just 2 OHT applications over a period of 1 week. The overall improvement of the BHI is based primarily on a significant increase in the reserve capacity and the maximum respiration of the mitochondria. The increase in non-mitochondrial oxygen consumption, which has a negative impact on the BHI value, is indicative for the Nrf-2 dependent activation of antioxidant and detoxifying enzymes activated through OHT. Conclusion These data demonstrate for the first time the beneficial effect of OHT on mitochondrial parameters. Thus, the results of this study suggest that OHT could be a safe and effective therapeutic option alone or as integrative and complementary support for pharmacological therapy in a variety of chronic and acute diseases where mitochondrial dysfunction plays a central role.

Comments on the optimal use of medical ozone in clinics versus the Ozone High Dose Therapy (OHT) approach

“…Considering that the mean blood pressure is about 127 mmHg (range 115-140 mmHg), i.e. 0.16 atm, and that the normal blood infusion speed is close to 15 drops/min, at 0.9 atm the number of blood drops might be approximately next to 84 drops/min, so falling within the fast reinfusion reported by our studies [1,25]. Moreover, we seriously wondered if adding oxygen to the reinfusion process at positive pressure does not cause embolism.…”

“…König and Lahodny published a paper where they endorsed a new approach of ozone therapy based on high dosage of this oxygen allotrope [1]. Ozone is a toxic xenobiotic for cells and produce toxic lipo-peroxides (LOPs), which only at defined and low dose ranges may exert a beneficial role on cells and organisms [13][14][15][16].…”

“…A first concern is that their conclusions cannot say anything about the therapeutic meaning of OHT, as the authors limited to assess that OHT improves mitochondria function in PBMCs, so practically investigating a simple in vitro model [1]. Moreover, they introduced an amazing hypothesis, fairly speculative: if OHT improves mitochondria functional parameters and mitochondria health is a foundation for a proper ozone-mediated therapy effect, then OHT might be considered a good alternative to the usual oxygen-ozone autohemotherapy (O 2 -O 3 -MAHT) with relatively low doses of ozone [1]. This might theoretically represent a good reasoning but lacks in vivo evidence and shows some rationale bias.…”

“…*Correspondence: salvatore.chirumbolo@univr.it the mitochondria functional parameters a) basal oxygen consumption rate (OCR), b) ATP-linked OCR and proton leak, c) maximal OCR and reserve capacity and d) non-mitochondrial OCR [1]. The Bioenergetic Health Index (BHI), the maximal Oxygen Consumption Rate (OCR), the reserve capacity OCR and the non-mitochondrial OCR, increased significantly following at least 2 sessions of OHT.…”

Ozone dialysis delivers three or more times the ozone than other forms of ozone blood treatment

“…It is commonly referred to as “10 pass” amongst American users. König and Lahodny 6 have recently shown that OHT stimulates mitochondria in blood mononuclear cells.…”