Ozone decontamination of bioclean rooms

Masaoka, T; Kubota, Yuki; Namiuchi, S; Takubo, T; Ueda, Tomoyuki; Shibata, Hiroki; Nakamura, Hideki; Yoshitake, J; Yamayoshi, Takao; Doi, Hitoshi; Kamiki, Teruo

doi:10.1128/aem.43.3.509-513.1982

Cited by 36 publications

(9 citation statements)

References 5 publications

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“…The same results were reported in several previous studies [ 22 – 24 , 50 , 55 – 57 ]. This might be attributed to the maximum concentration of gaseous O 3 used in this study is lower than the minimal bactericidal that has been recommended in earlier studies [ 51 , 52 ]. Moreover, the times of exposure for gaseous O 3 used in the present study were short comparing to earlier studies, where there is consensus that increase the concentration of gaseous O 3 exposure time in hours will increase the rate of bacterial reduction [ 50 , 58 ].…”

Section: Discussionmentioning

confidence: 63%

“…This finding is consistent with the results of other studies investigating the effect of concentration of O 3 that is dissolved in water on the reduction in MBP [ 25 , 30 – 31 , 49 – 50 ]. The minimal bactericidal concentration of O 3 is still an area of debate, where Hamelin and Chung [ 29 ], demonstrated that the minimum bactericidal concentration of O3 is 1 ppm, whilst other studies have recommended values between 3 ppm and 4 ppm [ 25 ; 31 , 50 ] and 20 to 30 ppm for gaseous O3 [ 51 , 52 ]. On the other hand, we didn’t identify significant impact exposure time on RF especially at higher concentrations except rubber.…”

Section: Discussionmentioning

confidence: 99%

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The microbial killing capacity of aqueous and gaseous ozone on different surfaces contaminated with dairy cattle manure

2018

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A high reactivity and leaving no harmful residues make ozone an effective disinfectant for farm hygiene and biosecurity. Our objectives were therefore to (1) characterize the killing capacity of aqueous and gaseous ozone at different operational conditions on dairy cattle manure-based pathogens (MBP) contaminated different surfaces (plastic, metal, nylon, rubber, and wood); (2) determine the effect of microbial load on the killing capacity of aqueous ozone. In a crossover design, 14 strips of each material were randomly assigned into 3 groups, treatment (n = 6), positive-control (n = 6), and negative-control (n = 2). The strips were soaked in dairy cattle manure with an inoculum level of 107–108 for 60 minutes. The treatment strips were exposed to aqueous ozone of 2, 4, and 9 ppm and gaseous ozone of 1and 9 ppm for 2, 4, and 8 minutes exposure. 3M™ Petrifilm™ rapid aerobic count plate and plate reader were used for bacterial culture. On smooth surfaces, plastic and metal, aqueous ozone at 4 ppm reduced MBP to a safe level (≥5-log10) within 2 minutes (6.1 and 5.1-log10, respectively). However, gaseous ozone at 9 ppm for 4 minutes inactivated 3.3-log10 of MBP. Aqueous ozone of 9 ppm is sufficient to reduce MBP to a safe level, 6.0 and 5.4- log10, on nylon and rubber surfaces within 2 and 8 minutes, respectively. On complex surfaces, wood, both aqueous and gaseous ozone at up to 9 ppm were unable to reduce MBP to a safe level (3.6 and 0.8-log10, respectively). The bacterial load was a strong predictor for reduction in MBP (P<0.0001, R2 = 0.72). We conclude that aqueous ozone of 4 and 9 ppm for 2 minutes may provide an efficient method to reduce MBP to a safe level on smooth and moderately rough surfaces, respectively. However, ozone alone may not an adequate means of controlling MBP on complex surfaces.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

The microbial killing capacity of aqueous and gaseous ozone on different surfaces contaminated with dairy cattle manure

2018

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“…The effectiveness of O3 for disinfecting surfaces and rooms has been examined in several previous studies, and many of them have used low O3 concentrations because of the toxic nature of the gas and its relatively long half-life [58,[83][84][85][86]. Nevertheless, the use of low O3 concentrations generally leads to limited or inconclusive results.…”

Section: Discussionmentioning

confidence: 99%

Detrimental Effect of Ozone on Pathogenic Bacteria

Rangel¹,

Cabral²,

Lechuga³

et al. 2021

Preprint

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(1) Background: Disinfection of medical devices designed for clinical use associated or not with the growing area of tissue engineering is an urgent need. However, traditional disinfection methods are not always suitable for some biomaterials, especially those sensitive to chemical, thermal, or radiation. Therefore, the objective of this study was to evaluate the minimal concentration of ozone gas (O3) necessary to control and kill a set of sensitive or multi-resistant Gram-positive and Gram-negative bacteria. The cell viability, membrane permeability, and the levels of reactive intracellular oxygen (ROS) species were also investigated; (2) Material and Methods: Four standard strains and a clinical MDR strain were exposed to low doses of ozone at different concentrations and times. Bacterial inactivation (cultivability, membrane damage) was investigated using colony counts, resazurin as a metabolic indicator, and propidium iodide (PI). A fluorescent probe (H2DCFDA) was used for the ROS analyses; (3) Results: No reduction in the count colony was detected after O3 exposure, compared to the control group. However, the cell viability of E. coli (30%), P. aeruginosa (25%), and A. baumannii (15%) was reduced considerably. The bacterial membrane of all strains was not affected by O3 but presented a significant increase of ROS in E. coli (90 ± 14%), P. aeruginosa (62.5 ± 19%), and A. baumanni (52.6 ± 5%); (4) Conclusion: Low doses of ozone were able to interfere in the cell viability of most strains studied, and although it does not cause damage to the bacterial membrane, increased levels of reactive ROS are responsible for causing a detrimental effect in the lipids, proteins, and DNA metabolism.

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“…The use of ozone gas for the inactivation of vegetative and spore-forming microorganisms on surfaces has been explored since at least 1982 [4]. More recently, ozone gas has been tested and is gaining traction for use in healthcare environments [5] for disinfection (in particular, inactivation of vegetative bacteria [6] and viruses [7]) and sterilization (inactivation of sporeforming microorganisms such as B. anthracis, Clostridioides difficile [8], and Bacillus cereus [6]).…”

Section: Introductionmentioning

confidence: 99%

The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials

et al. 2020

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A study was conducted to assess the efficacy of ozone gas in inactivating spores of both Bacillus anthracis and Bacillus subtilis inoculated onto six building materials (glass, wood, carpet, laminate, galvanized metal, and wallboard paper). Testing conditions consisted of ozone gas concentrations ranging from 7,000-12,000 parts per million (ppm), contact times from 4 to 12 h, and two relative humidity (RH) levels of 75 and 85%. Results showed that increasing the ozone concentration, contact time, and RH generally increased decontamination efficacy. The materials in which the highest decontamination efficacy was achieved for B. anthracis spores were wallboard paper, carpet, and wood with � 6 log 10 reduction (LR) occurring with 9,800 ppm ozone, 85% RH, for 6 h. The laminate and galvanized metal materials were generally more difficult to decontaminate, requiring 12,000 ppm ozone, 85% RH, and 9-12 h contact time to achieve �6 LR of B. anthracis. Lastly, overall, there were no significant differences in decontamination efficacy between the two species.

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Ozone decontamination of bioclean rooms

Cited by 36 publications

References 5 publications

The microbial killing capacity of aqueous and gaseous ozone on different surfaces contaminated with dairy cattle manure

The microbial killing capacity of aqueous and gaseous ozone on different surfaces contaminated with dairy cattle manure

Detrimental Effect of Ozone on Pathogenic Bacteria

The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials

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