Oxytocin reversed MK-801-induced social interaction and aggression deficits in zebrafish

Zimmermann, Fernanda Francine; Gaspary, Karina Vidarte; Siebel, Anna Maria; Bonan, Carla Denise

doi:10.1016/j.bbr.2016.05.059

Cited by 60 publications

(56 citation statements)

References 69 publications

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“…We observed that 5 and 10 µM MK-801 disrupted social interaction as measured by a decrease in the time zebrafish spent in the interaction zone. This result corroborates previous findings in other zebrafish studies (Seibt et al, 2011;Zimmermann et al, 2016) and in rodent models.…”

Section: Discussionsupporting

confidence: 93%

“…The exposure time and concentration curves were based in the literature (Kyzar et al, 2013;Tran et al, 2016;Zimmermann et al, 2016) and pilot studies from our group. The animals were allocated to the experimental groups following block randomization procedures to counterbalance the sex, the two different home tanks, and the test arenas between the groups.…”

Section: Experimental Designmentioning

confidence: 99%

“…In mice and rats, MK-801 causes hyperlocomotion, stereotypic behavior, decreased social interaction, sensorimotor gating deficits, and cognitive impairment (Jones et al, 2011). In zebrafish, a few studies have shown that MK-801 exposure causes hyperlocomotion (Franscescon et al, 2020;Menezes et al, 2015;Tran et al, 2016), social deficits (Zimmermann et al, 2016), and cognitive impairment (Cognato et al, 2012;Franscescon et al, 2020;Gaspary et al, 2018). The behavioral effects of AMPH administration, on the other hand, only recapitulate the positive symptoms of schizophrenia, which include delusions, hallucinations and agitation in humans (Krystal et al, 2005), and stereotypic behavior and hyperlocomotion in rodents (Featherstone et al, 2007;Tenn et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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How do zebrafish respond to MK-801 and amphetamine? Relevance for assessing schizophrenia-relevant endophenotypes in alternative model organisms

Benvenutti

Gallas‐Lopes

Sachett

et al. 2020

Preprint

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Background and Purpose: Schizophrenia pathophysiology has been associated with dopaminergic hyperactivity, loss of parvalbumin-positive GABAergic interneurons, NMDA receptor hypofunction, and redox dysregulation. Most behavioral assays and animal models to study this condition were developed in rodents, leaving room for species-specific biases that could be avoided by cross-species approaches. As MK-801 and amphetamine are largely used in mice and rats to mimic schizophrenia features, this study aimed to investigate the effects of these drugs in zebrafish. Experimental Approach: Adult zebrafish were exposed to MK-801 (1, 5, and 10 uM) or amphetamine (0.625, 2.5, and 10 mg.L-1) and observed in paradigms of locomotor activity and social behavior. Oxidative parameters relevant to schizophrenia were quantified in brain tissue. Key Results: MK-801 disrupted social interaction, an effect that resembles the negative symptoms of schizophrenia. It also altered locomotion in a context-dependent manner, with hyperactivity when fish were tested in the presence of social cues and hypoactivity when tested alone. On the other hand, exposure to amphetamine was devoid of effects on locomotion and social behavior, while increased lipid peroxidation in the brain. Conclusion and Implications: Key outcomes induced by MK-801 in rodents were replicated in zebrafish, which suggests this species is suitable as an alternative model animal to study psychotic disorders. More studies are necessary to further develop preclinical paradigms with this species and ultimately optimize the screening of potential novel treatments.

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Section: Discussionsupporting

confidence: 93%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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How do zebrafish respond to MK-801 and amphetamine? Relevance for assessing schizophrenia-relevant endophenotypes in alternative model organisms

Benvenutti

Gallas‐Lopes

Sachett

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…To validate the social preference assay, we treated zebrafish with either MK-801, an NMDA receptor antagonist that disrupts rodent (Moy et al, 2013) and zebrafish (Zimmermann et al, 2016) social behaviors, or DMSO vehicle control, for one hour prior to performing the behavioral assay. Animals treated with DMSO on average showed no spatial preference during the baseline period and a strong preference for conspecifics during the post-baseline period ( Figure S7C).…”

Section: Social Preference Assaymentioning

confidence: 99%

Inherited and De Novo Genetic Risk for Autism Impacts Shared Networks

Ruzzo

Pérez-Cano

Jung

et al. 2019

Cell

346

354

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“…Although, in some studies, the administration of OXT‐like peptides or its receptor agonist has shown to decrease or not to change social preference in cooperatively breeding cichlids, Neolamprologus pulcher and in zebrafish, other studies, using lower concentrations of these ligands, showed an effect in social preference that followed an inverted‐U dose‐response curve . Interestingly, the same highest OXT concentration that did not induce changes in sociality as reported in a study by Braida et al was also found to recover the deficits of social preference mediated by MK‐801 in an independent study without affecting the preference for the conspecifics when administered alone . Furthermore, although OXT‐like peptides do not increase zebrafish preference for the shoal, vasopressin‐like peptides do or exhibit the same dose‐dependent effect observed for OXT‐like peptides …”

Section: Introductionmentioning

confidence: 99%

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

Ribeiro

Nunes

Gliksberg

et al. 2020

J Neuroendocrinology

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| INTRODUC TI ONNonapeptides are known to be important players in the regulation of social behaviour across taxa. 1 They evolved from a common ancestral molecule, the arginine vasotocin, after a gene duplication event in early-jawed fish. 2 One copy originated the vasopressin-like peptides, which retained the most primitive functions of the ancestral molecule, namely the regulation of osmotic balance. The other originated the oxytocin (OXT)-like peptides, which have greatly diverged across species, being recruited for diverse homeostatic processes, including parturition and lactation in mammals. Throughout evolution, these nonapeptides have also been involved in the regulation of social behaviours, with vasopressin being more involved in aggression and agonistic behaviours and OXT in affiliative behaviours and sociality. 3 AbstractSociality is a complex phenomenon that involves the individual´s motivation to approach their conspecifics, along with social cognitive functions that enable individuals to interact and survive. The nonapeptide oxytocin (OXT) is known to regulate sociality in many species. However, the role of OXT in specific aspects of sociality is still not well understood. In the present study, we investigated the contribution of the OXT receptor (OXTR) signalling in two different aspects of zebrafish social behaviour: social preference, by measuring their motivation to approach a shoal of conspecifics, and social recognition, by measuring their ability to discriminate between a novel and familiar fish, using a mutant zebrafish lacking a functional OXTR. Although oxtr mutant zebrafish displayed normal attraction to a shoal of conspecifics, they exhibited reduced social recognition. We further investigated whether this effect would be social-domain specific by replacing conspecific fish by objects. Although no differences were observed in object approach, oxtr mutant fish also exhibited impaired object recognition. Our findings suggest that OXTR signalling regulates a more general memory recognition of familiar vs novel entities, not only in social but also in a non-social domain, in zebrafish. K E Y W O R D Sautism spectrum disorders, oxytocin, social preference, social recognition, zebrafish S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.

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Oxytocin reversed MK-801-induced social interaction and aggression deficits in zebrafish

Cited by 60 publications

References 69 publications

How do zebrafish respond to MK-801 and amphetamine? Relevance for assessing schizophrenia-relevant endophenotypes in alternative model organisms

How do zebrafish respond to MK-801 and amphetamine? Relevance for assessing schizophrenia-relevant endophenotypes in alternative model organisms

Inherited and De Novo Genetic Risk for Autism Impacts Shared Networks

Oxytocin receptor signalling modulates novelty recognition but not social preference in zebrafish

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