Oxytocin receptors expressed and coupled to Ca<sup>2+</sup> signalling in a human vascular smooth muscle cell line

Yazawa, Hidenori; Hirasawa, Akira; Horie, Kuniko; Saita, Yuji; Iida, Emi; Honda, Kazuo; Tsujimoto, Gozoh

doi:10.1111/j.1476-5381.1996.tb15263.x

Cited by 28 publications

(18 citation statements)

References 26 publications

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“…(16) suggested that oxytocin affects vascular smooth muscle cells in culture via Vi -vasopressin receptors, while Stassen et al (17 and Yazawa et al. (18) found specific oxytocin receptors in renal epithelia and vascular cell lines. Further, Yazawa eí oí.…”

Section: Introductionmentioning

confidence: 95%

“…Further, Yazawa eí oí. (18) showed that oxytocin-but not vasopressin-selective receptors are expressed and coupled to phosphatidylinositol turnover in a vascular smooth muscle cell line of the human. Despite these findings, however, the reason for the expression of oxytocin receptors in the peripheral 'non-target' tissues as well as the mechanisms of the effects of oxytocin on non-myometrial smooth muscle organs are still unclear.…”

Section: Introductionmentioning

confidence: 98%

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Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

Duridanova

Nedelcheva

Gagov

1997

European Journal of Endocrinology

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To study the effects of oxytocin on both spontaneous phasic contractions and K+ outward currents (IK) of the so-called 'non-target' smooth muscle cells, physiological concentrations of oxytocin ranging between 10(-12) mol/l and 10(-8) mol/l were applied to smooth muscle preparations and single voltage-clamped cells isolated from the circular layer of the guinea-pig gastric antrum. Oxytocin (10(-12) mol/l to 10(-8) mol/l) suppressed, in a dose-dependent manner, the tetrodotoxin- and atropine-resistant spontaneous phasic contractions and shifted rightward the dose-response curves of 10(-7) mol/l charybdotoxin and 10(-3) mol/l BaCl2. In cells with preloaded intracellular Ca2+ stores, oxytocin (10(-12) mol/l to 10(-9) mol/l) caused a dose-dependent activation of the charybdotoxin-blockable non-inactivating component of IK (IK(sl)) of single voltage-clamped cells, which was accompanied by hyperpolarization of the cell membranes. 8Lys-vasopressin and 8arg-vasopressin failed to mimic the effects of oxytocin on both contraction and K+ currents. Further, the oxytocin-induced activation of IK(sl) was effectively antagonized by 5 x 10(-8) mol/l U-73122 or 5 x 10(-6) mol/l 2-nitro-4-carboxyphenyl N,N-diphenylcarbamate (inhibitors of the cell membrane phospholipase C), as well as by intracellularly applied heparin (selective inhibitor of inositol-1,4,5-trisphosphate (IP3)-induced Ca2+ release channels). In cells incubated in the absence of Ca2+ entry throughout the study, oxytocin (10(-9) mol/l) caused a slight and transient increase of IK(sl) amplitudes. Neither ryanodine (10(-6) mol/l) nor cyclopiazonic acid (10(-6) mol/l) were able to restore the IK-activating effect of oxytocin in these cells. The data obtained suggest (i) that selective oxytocin receptors are present on the membranes of guinea-pig antral smooth muscle cells, (ii) that the oxytocin-related relaxation may result from the activation of Ca(2+)-sensitive K+ conductivity via activation of IP3-induced release of Ca2+ from the submembrane located cisternae of the sarcoplasmic reticulum Ca2+ stores and (iii) in turn, this evokes a non-inactivating component of IK, hyperpolarizing the cell membrane.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 98%

Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

Duridanova

Nedelcheva

Gagov

1997

European Journal of Endocrinology

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“…It is primarily essential for the stimulation of uterine smooth muscle contraction during labor and milk ejection during lactation [8]. OT exerts its biological activities through G-protein-coupled receptors, which are widely expressed in the central nervous system, endothelial and smooth muscle cells of blood vessels, kidney, heart, thymus, pancreas, and adipocytes [8,21,26,35]. More recently, OT receptors have been demonstrated in each developmental stage of follicles in human ovary [30].…”

Section: Introductionmentioning

confidence: 98%

Protective effect of oxytocin on ovarian ischemia-reperfusion injury in rats

et al. 2014

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“…Like V 1 Rs, OTRs are coupled to PLC, the metabolism of phosphoinositides, and the consequent elevation of intracellular calcium (7). In myometrial and mammary myoepithelial cells, OTR stimulation produces smooth muscle contraction (7), and this may also occur in vascular smooth muscle (17,18) (Fig. 2).…”

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confidence: 99%

Vasopressin: Mechanisms of action on the vasculature in health and in septic shock

Barrett

Singer²,

Clapp³

2007

Critical Care Medicine

199

129

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The pathophysiologic mechanism underlying vasopressin hypersensitivity in septic shock is probably multifactorial. It is doubtful that this phenomenon is merely the consequence of replacing a deficiency. Changes in vascular receptors or their signaling and/or interactions between vasopressin, nitric oxide, and adenosine triphosphate-dependent potassium channels are likely to be relevant. Further translational research is required to improve our understanding and direct appropriate educated clinical use of vasopressin.

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Oxytocin receptors expressed and coupled to Ca²⁺ signalling in a human vascular smooth muscle cell line

Cited by 28 publications

References 26 publications

Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

Protective effect of oxytocin on ovarian ischemia-reperfusion injury in rats

Vasopressin: Mechanisms of action on the vasculature in health and in septic shock

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Oxytocin receptors expressed and coupled to Ca2+ signalling in a human vascular smooth muscle cell line

Cited by 28 publications

References 26 publications

Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

Oxytocin-induced changes in single cell K+ currents and smooth muscle contraction of guinea-pig gastric antrum

Protective effect of oxytocin on ovarian ischemia-reperfusion injury in rats

Vasopressin: Mechanisms of action on the vasculature in health and in septic shock

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Product

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Oxytocin receptors expressed and coupled to Ca²⁺ signalling in a human vascular smooth muscle cell line