Oxytocin‐monomeric red fluorescent protein 1 synthesis in the hypothalamus under osmotic challenge and acute hypovolemia in a transgenic rat line

Ueno, Hiroki; Sanada, Kenya; Miyamoto, Tetsu; Baba, Kazuhiko; Tanaka, Kentaro; Nishimura, Haruki; Nishimura, Kazuaki; Sonoda, Shozo; Yoshimura, Mitsuhiro; Maruyama, Takashi; Oginosawa, Yasushi; Araki, Motoharu; Shinjo, Samuel Katsuyuki; Onaka, Tatsushi

doi:10.14814/phy2.14558

Cited by 2 publications

(1 citation statement)

References 71 publications

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“…To measure changes in the expression of the mRFP1 and OXT genes in the PVN and SON via in situ hybridisation histochemistry, transgenic rats were sacrificed by decapitation after hormone replacement in the state of OVX. We performed in situ hybridisation based on previous reports 61 , 62 . Brain tissue was frozen immediately and carefully using crushed dry ice after decapitation.…”

Section: Methodsmentioning

confidence: 99%

Oestrogen-dependent hypothalamic oxytocin expression with changes in feeding and body weight in female rats

et al. 2022

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Oxytocin (OXT) is produced in the hypothalamic nuclei and secreted into systemic circulation from the posterior pituitary gland. In the central nervous system, OXT regulates behaviours including maternal and feeding behaviours. Our aim is to evaluate whether oestrogen regulates hypothalamic OXT dynamics. Herein, we provide the first evidence that OXT dynamics in the hypothalamus vary with sex and that oestrogen may modulate dynamic changes in OXT levels, using OXT-mRFP1 transgenic rats. The fluorescence intensity of OXT-mRFP1 and expression of the OXT and mRFP1 genes in the hypothalamic nuclei is highest during the oestrus stage in female rats and decreased significantly in ovariectomised rats. Oestrogen replacement caused significant increases in fluorescence intensity and gene expression in a dose-related manner. This is also demonstrated in the rats’ feeding behaviour and hypothalamic Fos neurons using cholecystokinin-8 and immunohistochemistry. Hypothalamic OXT expression is oestrogen-dependent and can be enhanced centrally by the administration of oestrogen.

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Section: Methodsmentioning

confidence: 99%

Oestrogen-dependent hypothalamic oxytocin expression with changes in feeding and body weight in female rats

et al. 2022

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Navigating Central Oxytocin Transport: Known Realms and Uncharted Territories

Parmaksiz,

Kim

2024

Neuroscientist

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Complex mechanisms govern the transport and action of oxytocin (Oxt), a neuropeptide and hormone that mediates diverse physiologic processes. While Oxt exerts site-specific and rapid effects in the brain via axonal and somatodendritic release, volume transmission via CSF and the neurovascular interface can act as an additional mechanism to distribute Oxt signals across distant brain regions on a slower timescale. This review focuses on modes of Oxt transport and action in the CNS, with particular emphasis on the roles of perivascular spaces, the blood-brain barrier (BBB), and circumventricular organs in coordinating the triadic interaction among circulating blood, CSF, and parenchyma. Perivascular spaces, critical conduits for CSF flow, play a pivotal role in Oxt diffusion and distribution within the CNS and reciprocally undergo Oxt-mediated structural and functional reconstruction. While the BBB modulates the movement of Oxt between systemic and cerebral circulation in a majority of brain regions, circumventricular organs without a functional BBB can allow for diffusion, monitoring, and feedback regulation of bloodborne peripheral signals such as Oxt. Recognition of these additional transport mechanisms provides enhanced insight into the systemic propagation and regulation of Oxt activity.

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Oxytocin‐monomeric red fluorescent protein 1 synthesis in the hypothalamus under osmotic challenge and acute hypovolemia in a transgenic rat line

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 2 publications

References 71 publications

Oestrogen-dependent hypothalamic oxytocin expression with changes in feeding and body weight in female rats

Oestrogen-dependent hypothalamic oxytocin expression with changes in feeding and body weight in female rats

Navigating Central Oxytocin Transport: Known Realms and Uncharted Territories

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