Oxytocin Inhibits T-Type Calcium Current of Human Decidual Stromal Cells

Liu, Bo; Hill, Stephen J.; Khan, Raheela

doi:10.1210/jc.2005-0480

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…However, corroborating the modulatory effect of beta-estradiol exerted on voltage-gated calcium channels and on estrogen receptors, we might consider that there is an interplay between these key players that involves the activation of calcium signaling pathways. Other hormones (e.g., progesterone, oxytocin) have also been described to block voltage-gated calcium channels [ 62 , 63 , 64 ], and we might suppose a similar inhibition in TCs human myometrial preparations, but our study was focused only on estrogen effects.…”

Section: Discussionmentioning

confidence: 99%

Beta-Estradiol Regulates Voltage-Gated Calcium Channels and Estrogen Receptors in Telocytes from Human Myometrium

Banciu

Mustăciosu

et al. 2018

IJMS

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Voltage-gated calcium channels and estrogen receptors are essential players in uterine physiology, and their association with different calcium signaling pathways contributes to healthy and pathological conditions of the uterine myometrium. Among the properties of the various cell subtypes present in human uterine myometrium, there is increasing evidence that calcium oscillations in telocytes (TCs) contribute to contractile activity and pregnancy. Our study aimed to evaluate the effects of beta-estradiol on voltage-gated calcium channels and estrogen receptors in TCs from human uterine myometrium and to understand their role in pregnancy. For this purpose, we employed patch-clamp recordings, ratiometric Fura-2-based calcium imaging analysis, and qRT-PCR techniques for the analysis of cultured human myometrial TCs derived from pregnant and non-pregnant uterine samples. In human myometrial TCs from both non-pregnant and pregnant uterus, we evidenced by qRT-PCR the presence of genes encoding for voltage-gated calcium channels (Cav3.1, Ca3.2, Cav3.3, Cav2.1), estrogen receptors (ESR1, ESR2, GPR30), and nuclear receptor coactivator 3 (NCOA3). Pregnancy significantly upregulated Cav3.1 and downregulated Cav3.2, Cav3.3, ESR1, ESR2, and NCOA3, compared to the non-pregnant condition. Beta-estradiol treatment (24 h, 10, 100, 1000 nM) downregulated Cav3.2, Cav3.3, Cav1.2, ESR1, ESR2, GRP30, and NCOA3 in TCs from human pregnant uterine myometrium. We also confirmed the functional expression of voltage-gated calcium channels by patch-clamp recordings and calcium imaging analysis of TCs from pregnant human myometrium by perfusing with BAY K8644, which induced calcium influx through these channels. Additionally, we demonstrated that beta-estradiol (1000 nM) antagonized the effect of BAY K8644 (2.5 or 5 µM) in the same preparations. In conclusion, we evidenced the presence of voltage-gated calcium channels and estrogen receptors in TCs from non-pregnant and pregnant human uterine myometrium and their gene expression regulation by beta-estradiol in pregnant conditions. Further exploration of the calcium signaling in TCs and its modulation by estrogen hormones will contribute to the understanding of labor and pregnancy mechanisms and to the development of effective strategies to reduce the risk of premature birth.

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Section: Discussionmentioning

confidence: 99%

Beta-Estradiol Regulates Voltage-Gated Calcium Channels and Estrogen Receptors in Telocytes from Human Myometrium

Banciu

Mustăciosu

et al. 2018

IJMS

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“…These cells have the characteristics of myofibroblasts, including contractile activity [60], and thus may influence normal or abnormal delivery of the foetus. Isolated stromal cells exhibit inward Ca 2+ current that is predominantly T-type [61] as judged by a high sensitivity to Ni 2+ , but not to nifedipine, that furthermore is attenuated by oxytocin. In addition, oxytocin reduced the inward current, but the effect was greater in cells from women in labour, compared to those obtained at Caesarean section.…”

Section: Myometriummentioning

confidence: 99%

T-type Ca2+ channels in non-vascular smooth muscles

Fry

Sui

2006

Cell Calcium

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“…Placental OXT induces uterine contractions by directly affecting the myometrium as well as indirectly by increasing the production of prostaglandins, especially prostaglandin F2 α (PGF2 α), by the decidua, which upregulates OXTR expression and formation of gap junctions that also promote contractions [50]. OXT production and OXTR expression by decidual cells seem to be particularly important during labor, as defended in previous works [51,52]. The pathogenic implications of OXT and OXTR overexpression in FE-PW must be deeply studied.…”

Section: Discussionmentioning

confidence: 94%

Assessment of Tissue Expression of the Oxytocin–Vasopressin Pathway in the Placenta of Women with a First-Episode Psychosis during Pregnancy

Ortega,

García-Montero,

Fraile-Martinez

et al. 2023

IJMS

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Psychosis refers to a mental health condition characterized by a loss of touch with reality, comprising delusions, hallucinations, disorganized thought, disorganized behavior, catatonia, and negative symptoms. A first-episode psychosis (FEP) is a rare condition that can trigger adverse outcomes both for the mother and newborn. Previously, we demonstrated the existence of histopathological changes in the placenta of pregnant women who suffer an FEP in pregnancy. Altered levels of oxytocin (OXT) and vasopressin (AVP) have been detected in patients who manifested an FEP, whereas abnormal placental expression of these hormones and their receptors (OXTR and AVPR1A) has been proven in different obstetric complications. However, the precise role and expression of these components in the placenta of women after an FEP have not been studied yet. Thus, the purpose of the present study was to analyze the gene and protein expression, using RT-qPCR and immunohistochemistry (IHC), of OXT, OXTR, AVP, and AVPR1a in the placental tissue of pregnant women after an FEP in comparison to pregnant women without any health complication (HC-PW). Our results showed increased gene and protein expression of OXT, AVP, OXTR, and AVPR1A in the placental tissue of pregnant women who suffer an FEP. Therefore, our study suggests that an FEP during pregnancy may be associated with an abnormal paracrine/endocrine activity of the placenta, which can negatively affect the maternofetal wellbeing. Nevertheless, additional research is required to validate our findings and ascertain any potential implications of the observed alterations.

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Oxytocin Inhibits T-Type Calcium Current of Human Decidual Stromal Cells

Abstract: Inhibition of I(Ca(T)) by OT in decidual cells obtained during labor may signify important functional remodeling of uterine signaling during this period.

Cited by 6 publications

References 28 publications

Beta-Estradiol Regulates Voltage-Gated Calcium Channels and Estrogen Receptors in Telocytes from Human Myometrium

Beta-Estradiol Regulates Voltage-Gated Calcium Channels and Estrogen Receptors in Telocytes from Human Myometrium

T-type Ca2+ channels in non-vascular smooth muscles

Assessment of Tissue Expression of the Oxytocin–Vasopressin Pathway in the Placenta of Women with a First-Episode Psychosis during Pregnancy

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