Oxytocin enhances progesterone production with upregulation of BMP-15 activity by granulosa cells

Yamamoto, Koichiro; Nakano, Yasuhiro; Iwata, Nahoko; Soejima, Yoshiaki; Suyama, Atsuhito; Hasegawa, Tomohiko; Otsuka, Fumio

doi:10.1016/j.bbrc.2023.01.073

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…Primer pairs for PCR were determined from different exons to eliminate PCR products derived from chromosomal DNA. Primer pairs for steroidogenic acute regulatory protein (StAR), inhibitor of DNA binding 1 (Id-1), Smad6, Smad7, activin receptor-like kinase (ALK)-2 and -3, activin type-II receptor (ActRII), BMP type-II receptor (BMPRII), and ribosomal protein L19 (RPL19), a housekeeping gene, were prepared as we reported previously [ 54 ]. Primer pairs for cytochrome P450 family 11 subfamily B member 2 (CYP11B2), cytochrome P450 family 17 subfamily A member 1 (CYP17), and 3β-hydroxysteroid dehydrogenase type 1 (HSD3B1) were prepared according to other reports [ 31 , 33 ].…”

Section: Methodsmentioning

confidence: 99%

Interaction of Orexin and Bone Morphogenetic Proteins in Steroidogenesis by Human Adrenocortical Cells

Soejima

Iwata

Nishioka

et al. 2023

IJMS

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Orexins are neuropeptides that play important roles in sleep-wake regulation and food intake in the central nervous system, but their receptors are also expressed in peripheral tissues, including the endocrine system. In the present study, we investigated the functions of orexin in adrenal steroidogenesis using human adrenocortical H295R cells by focusing on its interaction with adrenocortical bone morphogenetic proteins (BMPs) that induce adrenocortical steroidogenesis. Treatment with orexin A increased the mRNA levels of steroidogenic enzymes including StAR, CYP11B2, CYP17, and HSD3B1, and these effects of orexin A were further enhanced in the presence of forskolin. Interestingly, orexin A treatment suppressed the BMP-receptor signaling detected by Smad1/5/9 phosphorylation and Id-1 expression through upregulation of inhibitory Smad7. Orexin A also suppressed endogenous BMP-6 expression but increased the expression of the type-II receptor of ActRII in H295R cells. Moreover, treatment with BMP-6 downregulated the mRNA level of OX1R, but not that of OX2R, expressed in H295R cells. In conclusion, the results indicate that both orexin and BMP-6 accelerate adrenocortical steroidogenesis in human adrenocortical cells; both pathways mutually inhibit each other, thereby leading to a fine-tuning of adrenocortical steroidogenesis.

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Section: Methodsmentioning

confidence: 99%

Interaction of Orexin and Bone Morphogenetic Proteins in Steroidogenesis by Human Adrenocortical Cells

Soejima

Iwata

Nishioka

et al. 2023

IJMS

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“…Interactions between OXT and estrogens have been proposed in both preclinical and clinical studies (e.g., [ 102 , 103 ]). Meanwhile, progesterone inhibits OXT binding; although, OXT accelerates follicle-stimulating hormone (FSH)- and forskolin (FSK)-induced progesterone production [ 104 , 105 ].…”

Section: Involvement Of Sex Hormonesmentioning

confidence: 99%

Exploring a Possible Interplay between Schizophrenia, Oxytocin, and Estrogens: A Narrative Review

2023

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Schizophrenia is characterized by symptoms of psychosis and sociocognitive deficits. Considering oxytocin’s antipsychotic and prosocial properties, numerous clinical, and preclinical studies have explored the neuropeptide’s therapeutic efficacy. Sex differences in the clinical course of schizophrenia, as well as in oxytocin-mediated behaviors, indicate the involvement of gonadal steroid hormones. The current narrative review aimed to explore empirical evidence on the interplay between schizophrenia psychopathology and oxytocin’s therapeutic potential in consideration of female gonadal steroid interactions, with a focus on estrogens. The review was conducted using the PubMed and PsychINFO databases and conforms to the Scale for the Assessment of Narrative Review Articles (SANRA) guidelines. The results suggest a potential synergistic effect of the combined antipsychotic effect of oxytocin and neuroprotective effect of estrogen on schizophrenia. Consideration of typical menstrual cycle-related hormonal changes is warranted and further research is needed to confirm this assumption.

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“…OT receptors (OXTR) are expressed in several body parts including the ovaries and prostate gland. Specifically, OXTR are found to be expressed in the granulosa cells, and in the small follicles in several mammal species including humans [21], and OT takes a still unclear role in steroidogenesis [22]. Despite the growing interest in the role played by oxytocin in several human functions, its possible role in one or more psychological symptoms, such as depression, anxiety, and social cognitive impairments or physical symptoms, such as metabolic dysfunctions or infertility, of PCOS [23] is still not clear or little studied.…”

Section: Introductionmentioning

confidence: 99%

The Role of Oxytocin in Polycystic Ovary Syndrome: A Systematic Review

Cera,

Pinto,

Pignatelli

2024

Preprint

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Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder that affects women of reproductive age representing the primary cause of anovulatory infertility. The nonapeptide oxytocin (OT) plays an important role in cognitive, emotional, and reproductive functions in human beings. Oxytocin receptors are expressed in several body parts including the ovaries. Despite this, the possible role played by oxytocin in symptoms of PCOS is not clear. The present systematic review aimed at understanding, the presence of possible oxytocin level alterations in PCOS, the connection between alterations of OT levels and the symptoms of PCOS, and the effect of oxytocin administration in PCOS. After a systematic search in the principal databases, seven studies, five human and two animal, were included. Most of the studies highlighted the role played by oxytocin in fertility issues related to PCOS. Studies that analyzed oxytocin basal levels in PCOS women agreed that PCOS is associated with a reduction in the serum level of oxytocin. Two studies found that in PCOS the serum levels of oxytocin were lower than healthy controls. One human and one animal study agreed about lower levels of PCOS, confirming a possible implication of a dysfunction of OT, in the pathogenesis of PCOS.

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Oxytocin enhances progesterone production with upregulation of BMP-15 activity by granulosa cells

Cited by 5 publications

References 36 publications

Interaction of Orexin and Bone Morphogenetic Proteins in Steroidogenesis by Human Adrenocortical Cells

Interaction of Orexin and Bone Morphogenetic Proteins in Steroidogenesis by Human Adrenocortical Cells

Exploring a Possible Interplay between Schizophrenia, Oxytocin, and Estrogens: A Narrative Review

The Role of Oxytocin in Polycystic Ovary Syndrome: A Systematic Review

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